Bio-Based Production of Dimethyl Itaconate From Rice Wine Waste-Derived Itaconic Acid

Young Chul Joo, Seung Kyou You, Sang Kyu Shin, Young Jin Ko, Ki Ho Jung, Sang A. Sim, Sung Ok Han

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Dimethyl itaconate is an important raw material for copolymerization, but it is not synthesized from itaconic acid by organisms. Moreover, Corynebacterium glutamicum is used as an important industrial host for the production of organic acids, but it does not metabolize itaconic acid. Therefore, the biosynthetic route toward dimethyl itaconate from itaconic acid is highly needed. In this study, a biological procedure for dimethyl itaconate production is developed from rice wine waste-derived itaconic acid using the engineered C. glutamicum strain. The first step is to investigate the effect of the co-overexpression of the codon-optimized cis-aconitic acid decarboxylase (CadA*) and a transcriptional regulator of genes involved in acetic acid metabolism (RamA) on itaconic acid production. The second step is to convert itaconic acid into dimethyl itaconate by lipase-catalyzed esterification. The CadA* and RamA-overexpressing CG4 strain increases the itaconic acid concentration under N-starvation with glucose and acetic acid compared with the concentration produced in the base mCGXII medium with glucose. Furthermore, the rice wine waste-derived itaconic acid is successfully converted into dimethyl itaconate using lipase from Rhizomucor miehei and a methanol substrate. This study is the first trial for bio-based production of dimethyl itaconate from rice wine waste-derived itaconic acid.

Original languageEnglish
JournalBiotechnology Journal
DOIs
Publication statusAccepted/In press - 2017

Fingerprint

Wine
NSC 153174
Aconitic Acid
Corynebacterium glutamicum
Carboxy-Lyases
Lipase
Acetic Acid
Rhizomucor
Oryza
itaconic acid
dimethyl itaconate
Glucose
Esterification
Regulator Genes
Starvation
Codon
Methanol
Acids

Keywords

  • Bio-based production
  • Corynebacterium glutamicum
  • Dimethyl itaconate
  • Itaconic acid
  • Rice wine waste

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Molecular Medicine

Cite this

Bio-Based Production of Dimethyl Itaconate From Rice Wine Waste-Derived Itaconic Acid. / Joo, Young Chul; You, Seung Kyou; Shin, Sang Kyu; Ko, Young Jin; Jung, Ki Ho; Sim, Sang A.; Han, Sung Ok.

In: Biotechnology Journal, 2017.

Research output: Contribution to journalArticle

Joo, Young Chul ; You, Seung Kyou ; Shin, Sang Kyu ; Ko, Young Jin ; Jung, Ki Ho ; Sim, Sang A. ; Han, Sung Ok. / Bio-Based Production of Dimethyl Itaconate From Rice Wine Waste-Derived Itaconic Acid. In: Biotechnology Journal. 2017.
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