Bioactive terpenoids from Euonymus verrucosus var. pauciflorus showing NO inhibitory activities

Yuling Yang, Xueyuan Yang, Xuke Zhang, Ziteng Song, Feng Liu, Yue Liang, Jie Zhang, Da Qing Jin, Jing Xu, Dongho Lee, Muhetaer Tuerhong, Yasushi Ohizumi, Yuanqiang Guo

Research output: Contribution to journalArticle

Abstract

In our continuous search for new nitric oxide (NO) inhibitory compounds as potential anti-inflammatory agents or lead compounds for inflammatory diseases, the chemical constituents of Euonymus verrucosus var. pauciflorus were investigated, leading to the isolation of eleven terpenoids including six new diterpenoids, designated as euonymupenes A–F. The structures were elucidated on the basis of NMR and ECD data analysis. Euonymupenes A, C, and F feature rare labdane-type norditerpenoid skeletons. The NO inhibitory effects were evaluated and all of the isolates were found to inhibit lipopolysaccharide (LPS)-induced NO production in murine microglial BV-2 cells. Western blotting analysis indicated that the most active compound (5) can regulate iNOS (inducible nitric oxide synthase) expression. The further molecular docking studies exhibited the affinities of bioactive compounds with iNOS.

Original languageEnglish
Pages (from-to)447-456
Number of pages10
JournalBioorganic Chemistry
Volume87
DOIs
Publication statusPublished - 2019 Jun 1

Fingerprint

Euonymus
Terpenes
Nitric Oxide
Diterpenes
Nitric Oxide Synthase Type II
Lead compounds
Skeleton
Lipopolysaccharides
Anti-Inflammatory Agents
Western Blotting
Nuclear magnetic resonance

Keywords

  • Celastraceae
  • Euonymus verrucosus var. pauciflorus
  • iNOS
  • NO inhibitory activities
  • Norditerpenoids

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Drug Discovery
  • Organic Chemistry

Cite this

Bioactive terpenoids from Euonymus verrucosus var. pauciflorus showing NO inhibitory activities. / Yang, Yuling; Yang, Xueyuan; Zhang, Xuke; Song, Ziteng; Liu, Feng; Liang, Yue; Zhang, Jie; Jin, Da Qing; Xu, Jing; Lee, Dongho; Tuerhong, Muhetaer; Ohizumi, Yasushi; Guo, Yuanqiang.

In: Bioorganic Chemistry, Vol. 87, 01.06.2019, p. 447-456.

Research output: Contribution to journalArticle

Yang, Y, Yang, X, Zhang, X, Song, Z, Liu, F, Liang, Y, Zhang, J, Jin, DQ, Xu, J, Lee, D, Tuerhong, M, Ohizumi, Y & Guo, Y 2019, 'Bioactive terpenoids from Euonymus verrucosus var. pauciflorus showing NO inhibitory activities', Bioorganic Chemistry, vol. 87, pp. 447-456. https://doi.org/10.1016/j.bioorg.2019.03.022
Yang, Yuling ; Yang, Xueyuan ; Zhang, Xuke ; Song, Ziteng ; Liu, Feng ; Liang, Yue ; Zhang, Jie ; Jin, Da Qing ; Xu, Jing ; Lee, Dongho ; Tuerhong, Muhetaer ; Ohizumi, Yasushi ; Guo, Yuanqiang. / Bioactive terpenoids from Euonymus verrucosus var. pauciflorus showing NO inhibitory activities. In: Bioorganic Chemistry. 2019 ; Vol. 87. pp. 447-456.
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