Abstract
In our continuous search for new nitric oxide (NO) inhibitory compounds as potential anti-inflammatory agents or lead compounds for inflammatory diseases, the chemical constituents of Euonymus verrucosus var. pauciflorus were investigated, leading to the isolation of eleven terpenoids including six new diterpenoids, designated as euonymupenes A–F. The structures were elucidated on the basis of NMR and ECD data analysis. Euonymupenes A, C, and F feature rare labdane-type norditerpenoid skeletons. The NO inhibitory effects were evaluated and all of the isolates were found to inhibit lipopolysaccharide (LPS)-induced NO production in murine microglial BV-2 cells. Western blotting analysis indicated that the most active compound (5) can regulate iNOS (inducible nitric oxide synthase) expression. The further molecular docking studies exhibited the affinities of bioactive compounds with iNOS.
| Original language | English |
|---|---|
| Pages (from-to) | 447-456 |
| Number of pages | 10 |
| Journal | Bioorganic Chemistry |
| Volume | 87 |
| DOIs | |
| Publication status | Published - 2019 Jun 1 |
Fingerprint
Keywords
- Celastraceae
- Euonymus verrucosus var. pauciflorus
- iNOS
- NO inhibitory activities
- Norditerpenoids
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Drug Discovery
- Organic Chemistry
Cite this
Bioactive terpenoids from Euonymus verrucosus var. pauciflorus showing NO inhibitory activities. / Yang, Yuling; Yang, Xueyuan; Zhang, Xuke; Song, Ziteng; Liu, Feng; Liang, Yue; Zhang, Jie; Jin, Da Qing; Xu, Jing; Lee, Dongho; Tuerhong, Muhetaer; Ohizumi, Yasushi; Guo, Yuanqiang.
In: Bioorganic Chemistry, Vol. 87, 01.06.2019, p. 447-456.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Bioactive terpenoids from Euonymus verrucosus var. pauciflorus showing NO inhibitory activities
AU - Yang, Yuling
AU - Yang, Xueyuan
AU - Zhang, Xuke
AU - Song, Ziteng
AU - Liu, Feng
AU - Liang, Yue
AU - Zhang, Jie
AU - Jin, Da Qing
AU - Xu, Jing
AU - Lee, Dongho
AU - Tuerhong, Muhetaer
AU - Ohizumi, Yasushi
AU - Guo, Yuanqiang
PY - 2019/6/1
Y1 - 2019/6/1
N2 - In our continuous search for new nitric oxide (NO) inhibitory compounds as potential anti-inflammatory agents or lead compounds for inflammatory diseases, the chemical constituents of Euonymus verrucosus var. pauciflorus were investigated, leading to the isolation of eleven terpenoids including six new diterpenoids, designated as euonymupenes A–F. The structures were elucidated on the basis of NMR and ECD data analysis. Euonymupenes A, C, and F feature rare labdane-type norditerpenoid skeletons. The NO inhibitory effects were evaluated and all of the isolates were found to inhibit lipopolysaccharide (LPS)-induced NO production in murine microglial BV-2 cells. Western blotting analysis indicated that the most active compound (5) can regulate iNOS (inducible nitric oxide synthase) expression. The further molecular docking studies exhibited the affinities of bioactive compounds with iNOS.
AB - In our continuous search for new nitric oxide (NO) inhibitory compounds as potential anti-inflammatory agents or lead compounds for inflammatory diseases, the chemical constituents of Euonymus verrucosus var. pauciflorus were investigated, leading to the isolation of eleven terpenoids including six new diterpenoids, designated as euonymupenes A–F. The structures were elucidated on the basis of NMR and ECD data analysis. Euonymupenes A, C, and F feature rare labdane-type norditerpenoid skeletons. The NO inhibitory effects were evaluated and all of the isolates were found to inhibit lipopolysaccharide (LPS)-induced NO production in murine microglial BV-2 cells. Western blotting analysis indicated that the most active compound (5) can regulate iNOS (inducible nitric oxide synthase) expression. The further molecular docking studies exhibited the affinities of bioactive compounds with iNOS.
KW - Celastraceae
KW - Euonymus verrucosus var. pauciflorus
KW - iNOS
KW - NO inhibitory activities
KW - Norditerpenoids
UR - http://www.scopus.com/inward/record.url?scp=85063353003&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85063353003&partnerID=8YFLogxK
U2 - 10.1016/j.bioorg.2019.03.022
DO - 10.1016/j.bioorg.2019.03.022
M3 - Article
VL - 87
SP - 447
EP - 456
JO - Bioorganic Chemistry
JF - Bioorganic Chemistry
SN - 0045-2068
ER -