Bioactive terpenoids from Euonymus verrucosus var. pauciflorus showing NO inhibitory activities

Yuling Yang; Xueyuan Yang; Xuke Zhang; Ziteng Song; Feng Liu; Yue Liang; Jie Zhang; Da Qing Jin; Jing Xu; Dongho Lee; Muhetaer Tuerhong; Yasushi Ohizumi; Yuanqiang Guo

doi:10.1016/j.bioorg.2019.03.022

Bioactive terpenoids from Euonymus verrucosus var. pauciflorus showing NO inhibitory activities

Yuling Yang, Xueyuan Yang, Xuke Zhang, Ziteng Song, Feng Liu, Yue Liang, Jie Zhang, Da Qing Jin, Jing Xu, Dongho Lee, Muhetaer Tuerhong, Yasushi Ohizumi, Yuanqiang Guo

Department of Biosystems and Biotechnology

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

In our continuous search for new nitric oxide (NO) inhibitory compounds as potential anti-inflammatory agents or lead compounds for inflammatory diseases, the chemical constituents of Euonymus verrucosus var. pauciflorus were investigated, leading to the isolation of eleven terpenoids including six new diterpenoids, designated as euonymupenes A–F. The structures were elucidated on the basis of NMR and ECD data analysis. Euonymupenes A, C, and F feature rare labdane-type norditerpenoid skeletons. The NO inhibitory effects were evaluated and all of the isolates were found to inhibit lipopolysaccharide (LPS)-induced NO production in murine microglial BV-2 cells. Western blotting analysis indicated that the most active compound (5) can regulate iNOS (inducible nitric oxide synthase) expression. The further molecular docking studies exhibited the affinities of bioactive compounds with iNOS.

Original language	English
Pages (from-to)	447-456
Number of pages	10
Journal	Bioorganic Chemistry
Volume	87
DOIs	https://doi.org/10.1016/j.bioorg.2019.03.022
Publication status	Published - 2019 Jun

Keywords

Celastraceae
Euonymus verrucosus var. pauciflorus
NO inhibitory activities
Norditerpenoids
iNOS

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Drug Discovery
Organic Chemistry

Access to Document

10.1016/j.bioorg.2019.03.022

Fingerprint Dive into the research topics of 'Bioactive terpenoids from Euonymus verrucosus var. pauciflorus showing NO inhibitory activities'. Together they form a unique fingerprint.

Cite this

Bioactive terpenoids from Euonymus verrucosus var. pauciflorus showing NO inhibitory activities. / Yang, Yuling; Yang, Xueyuan; Zhang, Xuke; Song, Ziteng; Liu, Feng; Liang, Yue; Zhang, Jie; Jin, Da Qing; Xu, Jing; Lee, Dongho; Tuerhong, Muhetaer; Ohizumi, Yasushi; Guo, Yuanqiang.

In: Bioorganic Chemistry, Vol. 87, 06.2019, p. 447-456.

Research output: Contribution to journal › Article › peer-review

@article{72fabf1d161a465b8b55658782714a80,

title = "Bioactive terpenoids from Euonymus verrucosus var. pauciflorus showing NO inhibitory activities",

abstract = "In our continuous search for new nitric oxide (NO) inhibitory compounds as potential anti-inflammatory agents or lead compounds for inflammatory diseases, the chemical constituents of Euonymus verrucosus var. pauciflorus were investigated, leading to the isolation of eleven terpenoids including six new diterpenoids, designated as euonymupenes A–F. The structures were elucidated on the basis of NMR and ECD data analysis. Euonymupenes A, C, and F feature rare labdane-type norditerpenoid skeletons. The NO inhibitory effects were evaluated and all of the isolates were found to inhibit lipopolysaccharide (LPS)-induced NO production in murine microglial BV-2 cells. Western blotting analysis indicated that the most active compound (5) can regulate iNOS (inducible nitric oxide synthase) expression. The further molecular docking studies exhibited the affinities of bioactive compounds with iNOS.",

keywords = "Celastraceae, Euonymus verrucosus var. pauciflorus, NO inhibitory activities, Norditerpenoids, iNOS",

author = "Yuling Yang and Xueyuan Yang and Xuke Zhang and Ziteng Song and Feng Liu and Yue Liang and Jie Zhang and Jin, {Da Qing} and Jing Xu and Dongho Lee and Muhetaer Tuerhong and Yasushi Ohizumi and Yuanqiang Guo",

year = "2019",

month = jun,

doi = "10.1016/j.bioorg.2019.03.022",

language = "English",

volume = "87",

pages = "447--456",

journal = "Bioorganic Chemistry",

issn = "0045-2068",

publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Bioactive terpenoids from Euonymus verrucosus var. pauciflorus showing NO inhibitory activities

AU - Yang, Yuling

AU - Yang, Xueyuan

AU - Zhang, Xuke

AU - Song, Ziteng

AU - Liu, Feng

AU - Liang, Yue

AU - Zhang, Jie

AU - Jin, Da Qing

AU - Xu, Jing

AU - Lee, Dongho

AU - Tuerhong, Muhetaer

AU - Ohizumi, Yasushi

AU - Guo, Yuanqiang

PY - 2019/6

Y1 - 2019/6

N2 - In our continuous search for new nitric oxide (NO) inhibitory compounds as potential anti-inflammatory agents or lead compounds for inflammatory diseases, the chemical constituents of Euonymus verrucosus var. pauciflorus were investigated, leading to the isolation of eleven terpenoids including six new diterpenoids, designated as euonymupenes A–F. The structures were elucidated on the basis of NMR and ECD data analysis. Euonymupenes A, C, and F feature rare labdane-type norditerpenoid skeletons. The NO inhibitory effects were evaluated and all of the isolates were found to inhibit lipopolysaccharide (LPS)-induced NO production in murine microglial BV-2 cells. Western blotting analysis indicated that the most active compound (5) can regulate iNOS (inducible nitric oxide synthase) expression. The further molecular docking studies exhibited the affinities of bioactive compounds with iNOS.

AB - In our continuous search for new nitric oxide (NO) inhibitory compounds as potential anti-inflammatory agents or lead compounds for inflammatory diseases, the chemical constituents of Euonymus verrucosus var. pauciflorus were investigated, leading to the isolation of eleven terpenoids including six new diterpenoids, designated as euonymupenes A–F. The structures were elucidated on the basis of NMR and ECD data analysis. Euonymupenes A, C, and F feature rare labdane-type norditerpenoid skeletons. The NO inhibitory effects were evaluated and all of the isolates were found to inhibit lipopolysaccharide (LPS)-induced NO production in murine microglial BV-2 cells. Western blotting analysis indicated that the most active compound (5) can regulate iNOS (inducible nitric oxide synthase) expression. The further molecular docking studies exhibited the affinities of bioactive compounds with iNOS.

KW - Celastraceae

KW - Euonymus verrucosus var. pauciflorus

KW - NO inhibitory activities

KW - Norditerpenoids

KW - iNOS

UR - http://www.scopus.com/inward/record.url?scp=85063353003&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85063353003&partnerID=8YFLogxK

U2 - 10.1016/j.bioorg.2019.03.022

DO - 10.1016/j.bioorg.2019.03.022

M3 - Article

C2 - 30925429

AN - SCOPUS:85063353003

VL - 87

SP - 447

EP - 456

JO - Bioorganic Chemistry

JF - Bioorganic Chemistry

SN - 0045-2068

ER -