Bioactive terpenoids from Euonymus verrucosus var. pauciflorus showing NO inhibitory activities

Yuling Yang; Xueyuan Yang; Xuke Zhang; Ziteng Song; Feng Liu; Yue Liang; Jie Zhang; Da Qing Jin; Jing Xu; Dongho Lee; Muhetaer Tuerhong; Yasushi Ohizumi; Yuanqiang Guo

doi:10.1016/j.bioorg.2019.03.022

Bioactive terpenoids from Euonymus verrucosus var. pauciflorus showing NO inhibitory activities

Yuling Yang, Xueyuan Yang, Xuke Zhang, Ziteng Song, Feng Liu, Yue Liang, Jie Zhang, Da Qing Jin, Jing Xu, Dongho Lee, Muhetaer Tuerhong, Yasushi Ohizumi, Yuanqiang Guo

Division of Biotechnology

Research output: Contribution to journal › Article

1 Citation (Scopus)

Abstract

In our continuous search for new nitric oxide (NO) inhibitory compounds as potential anti-inflammatory agents or lead compounds for inflammatory diseases, the chemical constituents of Euonymus verrucosus var. pauciflorus were investigated, leading to the isolation of eleven terpenoids including six new diterpenoids, designated as euonymupenes A–F. The structures were elucidated on the basis of NMR and ECD data analysis. Euonymupenes A, C, and F feature rare labdane-type norditerpenoid skeletons. The NO inhibitory effects were evaluated and all of the isolates were found to inhibit lipopolysaccharide (LPS)-induced NO production in murine microglial BV-2 cells. Western blotting analysis indicated that the most active compound (5) can regulate iNOS (inducible nitric oxide synthase) expression. The further molecular docking studies exhibited the affinities of bioactive compounds with iNOS.

Original language	English
Pages (from-to)	447-456
Number of pages	10
Journal	Bioorganic Chemistry
Volume	87
DOIs	https://doi.org/10.1016/j.bioorg.2019.03.022
Publication status	Published - 2019 Jun 1

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Keywords

Celastraceae
Euonymus verrucosus var. pauciflorus
iNOS
NO inhibitory activities
Norditerpenoids

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Drug Discovery
Organic Chemistry

Cite this

Yang, Y., Yang, X., Zhang, X., Song, Z., Liu, F., Liang, Y., Zhang, J., Jin, D. Q., Xu, J., Lee, D., Tuerhong, M., Ohizumi, Y., & Guo, Y. (2019). Bioactive terpenoids from Euonymus verrucosus var. pauciflorus showing NO inhibitory activities. Bioorganic Chemistry, 87, 447-456. https://doi.org/10.1016/j.bioorg.2019.03.022

Bioactive terpenoids from Euonymus verrucosus var. pauciflorus showing NO inhibitory activities. / Yang, Yuling; Yang, Xueyuan; Zhang, Xuke; Song, Ziteng; Liu, Feng; Liang, Yue; Zhang, Jie; Jin, Da Qing; Xu, Jing; Lee, Dongho; Tuerhong, Muhetaer; Ohizumi, Yasushi; Guo, Yuanqiang.

In: Bioorganic Chemistry, Vol. 87, 01.06.2019, p. 447-456.

Research output: Contribution to journal › Article

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abstract = "In our continuous search for new nitric oxide (NO) inhibitory compounds as potential anti-inflammatory agents or lead compounds for inflammatory diseases, the chemical constituents of Euonymus verrucosus var. pauciflorus were investigated, leading to the isolation of eleven terpenoids including six new diterpenoids, designated as euonymupenes A–F. The structures were elucidated on the basis of NMR and ECD data analysis. Euonymupenes A, C, and F feature rare labdane-type norditerpenoid skeletons. The NO inhibitory effects were evaluated and all of the isolates were found to inhibit lipopolysaccharide (LPS)-induced NO production in murine microglial BV-2 cells. Western blotting analysis indicated that the most active compound (5) can regulate iNOS (inducible nitric oxide synthase) expression. The further molecular docking studies exhibited the affinities of bioactive compounds with iNOS.",

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AU - Liu, Feng

AU - Liang, Yue

AU - Zhang, Jie

AU - Jin, Da Qing

AU - Xu, Jing

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AU - Guo, Yuanqiang

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10.1016/j.bioorg.2019.03.022