Bioaugmentation of Methanosarcina thermophila grown on biochar particles during semi-continuous thermophilic food waste anaerobic digestion under two different bioaugmentation regimes

Jonathan T.E. Lee, Nalok Dutta, Le Zhang, Thomas T.H. Tsui, Shuhan Lim, Zhi Kai Tio, Ee Yang Lim, Jiachen Sun, Jingxin Zhang, Chi Hwa Wang, Yong Sik Ok, Birgitte K. Ahring, Yen Wah Tong

Research output: Contribution to journalArticlepeer-review

Abstract

This study presents the effect of bioaugmentation of thermophilic anaerobic digestion of food waste with Methanosarcina thermophila grown on a wood-derived biochar. Two different supplementation regimes were tested, namely a single bioaugmentation (SBABC) in which 10% v/v of the microbes grown on biochar (1 g/L) is added at setup of the reactors, versus a routine bioaugmentation (RBABC) wherein the same amount of supplements were added over 10 feeding cycles. The optimally performing ‘R’ and ‘S’ reactors had increased methane yields by 37% and 32% over their respective controls while reactors SBABC 2 and 3 produced 21.89% and 56.09% higher average methane yield than RBABC 2 and 3, respectively. It appears that a single dose bioaugmentation is advantageous for improving AD as analysed in terms of average methane yield and VFA production. This study provides the basis for understanding how biochar and bioaugmentation can be used for engineering sustainable pilot-scale AD processes.

Original languageEnglish
Article number127590
JournalBioresource technology
Volume360
DOIs
Publication statusPublished - 2022 Sep

Keywords

  • Bioaugmentation
  • Biochar
  • Food waste
  • Growth support particles
  • Thermophilic anaerobic digestion

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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