Biochemical and structural analysis of hormone-sensitive lipase homolog EstE7: Insight into the stabilized dimerization of HSL-homolog proteins

Ki Hyun Nam, Sung Ha Park, Won Ho Lee, Kwang Yeon Hwang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Hormone sensitive lipase (HSL) plays a major role in energy homeostasis and lipid metabolism. Several crystal structures of HSL-homolog proteins have been identified, which has led to a better understanding of its molecular function. HSL-homolog proteins exit as both monomer and dimer, but the biochemical and structural basis for such oligomeric states has not been successfully elucidated. Therefore, we determined the crystal structure of HSL-homolog protein EstE7 from a metagenome library at 2.2 Å resolution and characterized the oligomeric states of EstE7 both structurally and biochemically. EstE7 protein prefers the dimeric state in solution, which is supported by its higher enzymatic activity in the dimeric state. In the crystal form, EstE7 protein shows two-types of dimeric interface. Specifically, dimerization via the external β8-strand occurred through tight association between two pseudosymmetric folds via salt bridges, hydrogen bonds and van der Waals interactions. This dimer formation was similar to that of other HSL-homolog protein structures such as AFEST, BEFA, and EstE1. We anticipate that our results will provide insight into the oligomeric state of HSL-homolog proteins.

Original languageEnglish
Pages (from-to)2627-2632
Number of pages6
JournalBulletin of the Korean Chemical Society
Volume31
Issue number9
DOIs
Publication statusPublished - 2010 Sep 20

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Sterol Esterase
Dimerization
Structural analysis
Proteins
Dimers
Crystal structure
Hydrogen bonds
Salts
Monomers
Association reactions
Crystals

Keywords

  • Crystal structure
  • Dimerization
  • EstE7
  • HSL-homolog protein
  • Oligomeric state

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Biochemical and structural analysis of hormone-sensitive lipase homolog EstE7 : Insight into the stabilized dimerization of HSL-homolog proteins. / Nam, Ki Hyun; Park, Sung Ha; Lee, Won Ho; Hwang, Kwang Yeon.

In: Bulletin of the Korean Chemical Society, Vol. 31, No. 9, 20.09.2010, p. 2627-2632.

Research output: Contribution to journalArticle

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