Biochemical characterization of an extracellular β-glucosidase from the fungus, Penicillium italicum, Isolated from rotten citrus peel

Ah Reum Park, Joo Hee Hong, Jae-Jin Kim, Jeong Jun Yoon

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A β-glucosidase from Penicillium italicum was purified with a specific activity of 61.8 U/mg, using a chromatography system. The native form of the enzyme was an 88.5-kDa tetramer with a molecular mass of 354 kDa. Optimum activity was observed at pH 4.5 and 60°C, and the half-lives were 1,737, 330, 34, and 1 hr at 50, 55, 60, and 65°C, respectively. Its activity was inhibited by 47% by 5 mM Ni2+. The enzyme exhibited hydrolytic activity for p-nitrophenyl-β-D-glucopyranoside (pNP-Glu), p-nitrophenyl-β-D-cellobioside, p-nitrophenyl-β-D-xyloside, and cellobiose, however, no activity was observed for p-nitrophenyl-β-D-lactopyranoside, p-nitrophenyl-β-D-galactopyranoside, carboxymetyl cellulose, xylan, and cellulose, indicating that the enzyme was a β-glucosidase. The kcat/Km (s-1 mM-1) values for pNP-Glu and cellobiose were 15,770.4 mM and 6,361.4 mM, respectively. These values were the highest reported for β-glucosidases. Non-competitive inhibition of the enzyme by both glucose (Ki = 8.9 mM) and glucono-δ-lactone (Ki = 11.3 mM) was observed when pNP-Glu was used as the substrate. This is the first report of non-competitive inhibition of β-glucosidase by glucose and glucono-δ-lactone.

Original languageEnglish
Pages (from-to)173-180
Number of pages8
JournalMycobiology
Volume40
Issue number3
DOIs
Publication statusPublished - 2012 Dec 1

Fingerprint

Penicillium italicum
citrus peels
Glucosidases
glucosidases
Citrus
Penicillium
Fungi
Cellobiose
cellobiose
fungi
Lactones
Enzymes
lactones
Cellulose
cellulose
enzymes
Glucose
Xylans
glucose
enzyme inhibition

Keywords

  • β-Glucosidase
  • Cellulolytic fungi
  • Characterization
  • Identification
  • Purification

ASJC Scopus subject areas

  • Plant Science
  • Microbiology
  • Infectious Diseases

Cite this

Biochemical characterization of an extracellular β-glucosidase from the fungus, Penicillium italicum, Isolated from rotten citrus peel. / Park, Ah Reum; Hong, Joo Hee; Kim, Jae-Jin; Yoon, Jeong Jun.

In: Mycobiology, Vol. 40, No. 3, 01.12.2012, p. 173-180.

Research output: Contribution to journalArticle

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