Biochemical characterization of ferredoxin-NADP+reductase interaction with flavodoxin in Pseudomonas putida

Jinki Yeom, Woojun Park

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Flavodoxin (Fld) has been demonstrated to bind to ferredoxin-NADP+ reductase A (FprA) in Pseudomonas putida. Two residues (Phe256, Lys259) of FprA are likely to be important for interacting with Fld based on homology modeling. Sitedirected mutagenesis and pH-dependent enzyme kinetics were performed to further examine the role of these residues. The catalytic efficiencies of FprA-Ala259 and FprA-Asp259 proteins were two-fold lower than those of the wild-type FprA. Homology modeling also strongly suggested that these two residues are important for electron transfer. Thermodynamic properties such as entropy, enthalpy, and heat capacity changes of FprA-Ala259 and FprA-Asp259 were examined by isothermal titration calorimetry. We demonstrated, for the first time, that Phe256 and Lys259 are critical residues for the interaction between FprA and Fld. Van der Waals interactions and hydrogen bonding were also more important than ionic interactions for forming the FprA-Fld complex.

Original languageEnglish
Pages (from-to)476-481
Number of pages6
JournalBMB reports
Volume45
Issue number8
DOIs
Publication statusPublished - 2012 Aug

Keywords

  • Ferredoxin-NADP reductase
  • Flavodoxin
  • Isothermal titration calorimetry
  • Protein-protein interaction
  • Pseudomonas putida KT2440

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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