Biocompatibility, cellular uptake and biodistribution of the polymeric amphiphilic nanoparticles as oral drug carriers

Ya Liu, Ming Kong, Chao Feng, Kui Kun Yang, Yang Li, Jing Su, Xiao Jie Cheng, Hyun Jin Park, Xi Guang Chen

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Oleoyl-carboxymethyl-chitosan (OCMCS) was synthesized and were soluble at neutral pH. The critical micelle concentration (CMC) of OCMCS in deionized water was 0.021. mg/ml. OCMCS nanoparticles were successfully prepared via self-assembly with mean diameter of 215.34. nm, zeta potential of 19.26. mV and an almost spherical shape as determined by electron microscopy. The OCMCS nanoparticles showed low erythrocyte membrane-damaging effect. The MTT survival assay indicated no significant cytotoxicity to Caco-2 cells and MEFs cells. The uptake of FITC labeled OCMCS nanoparticles by Caco-2 cells was confirmed via confocal laser scanning microscope (CLSM). In vivo toxicity assays were performed via histopathological evaluation, and no specific anatomical pathological changes or tissue damage was observed in the tissues of carps. The extent of tissue distribution and retention following oral administration of FITC-OCMCS nanoparticles was analyzed for 3 days. After 3 days, the nanoparticles remained detectable in the muscle, heart, kidney, liver, intestine, and spleen. The results showed that 34.32% of the particles were localized in the liver, 18.79% in the kidney, and 17.36% in the heart. The lowest percentage was observed in the muscle. These results implied that OCMCS nanoparticles had great potential to be applied as safe carriers for the oral administration of protein drugs.

Original languageEnglish
Pages (from-to)345-353
Number of pages9
JournalColloids and Surfaces B: Biointerfaces
Volume103
DOIs
Publication statusPublished - 2013 Mar 1

Fingerprint

Drug Carriers
biocompatibility
Biocompatibility
Chitosan
Nanoparticles
drugs
nanoparticles
kidneys
muscles
Caco-2 Cells
Fluorescein-5-isothiocyanate
liver
Tissue
Liver
Oral Administration
cells
Muscle
Assays
intestines
spleen

Keywords

  • Biocompatibility
  • Biodistribution
  • Cellular uptake
  • Nanoparticles
  • OCMCS

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Biocompatibility, cellular uptake and biodistribution of the polymeric amphiphilic nanoparticles as oral drug carriers. / Liu, Ya; Kong, Ming; Feng, Chao; Yang, Kui Kun; Li, Yang; Su, Jing; Cheng, Xiao Jie; Park, Hyun Jin; Chen, Xi Guang.

In: Colloids and Surfaces B: Biointerfaces, Vol. 103, 01.03.2013, p. 345-353.

Research output: Contribution to journalArticle

Liu, Ya ; Kong, Ming ; Feng, Chao ; Yang, Kui Kun ; Li, Yang ; Su, Jing ; Cheng, Xiao Jie ; Park, Hyun Jin ; Chen, Xi Guang. / Biocompatibility, cellular uptake and biodistribution of the polymeric amphiphilic nanoparticles as oral drug carriers. In: Colloids and Surfaces B: Biointerfaces. 2013 ; Vol. 103. pp. 345-353.
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