Abstract
Oleoyl-carboxymethyl-chitosan (OCMCS) was synthesized and were soluble at neutral pH. The critical micelle concentration (CMC) of OCMCS in deionized water was 0.021. mg/ml. OCMCS nanoparticles were successfully prepared via self-assembly with mean diameter of 215.34. nm, zeta potential of 19.26. mV and an almost spherical shape as determined by electron microscopy. The OCMCS nanoparticles showed low erythrocyte membrane-damaging effect. The MTT survival assay indicated no significant cytotoxicity to Caco-2 cells and MEFs cells. The uptake of FITC labeled OCMCS nanoparticles by Caco-2 cells was confirmed via confocal laser scanning microscope (CLSM). In vivo toxicity assays were performed via histopathological evaluation, and no specific anatomical pathological changes or tissue damage was observed in the tissues of carps. The extent of tissue distribution and retention following oral administration of FITC-OCMCS nanoparticles was analyzed for 3 days. After 3 days, the nanoparticles remained detectable in the muscle, heart, kidney, liver, intestine, and spleen. The results showed that 34.32% of the particles were localized in the liver, 18.79% in the kidney, and 17.36% in the heart. The lowest percentage was observed in the muscle. These results implied that OCMCS nanoparticles had great potential to be applied as safe carriers for the oral administration of protein drugs.
| Original language | English |
|---|---|
| Pages (from-to) | 345-353 |
| Number of pages | 9 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 103 |
| DOIs | |
| Publication status | Published - 2013 Mar 1 |
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Keywords
- Biocompatibility
- Biodistribution
- Cellular uptake
- Nanoparticles
- OCMCS
ASJC Scopus subject areas
- Biotechnology
- Colloid and Surface Chemistry
- Physical and Theoretical Chemistry
- Surfaces and Interfaces
Cite this
Biocompatibility, cellular uptake and biodistribution of the polymeric amphiphilic nanoparticles as oral drug carriers. / Liu, Ya; Kong, Ming; Feng, Chao; Yang, Kui Kun; Li, Yang; Su, Jing; Cheng, Xiao Jie; Park, Hyun Jin; Chen, Xi Guang.
In: Colloids and Surfaces B: Biointerfaces, Vol. 103, 01.03.2013, p. 345-353.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biocompatibility, cellular uptake and biodistribution of the polymeric amphiphilic nanoparticles as oral drug carriers
AU - Liu, Ya
AU - Kong, Ming
AU - Feng, Chao
AU - Yang, Kui Kun
AU - Li, Yang
AU - Su, Jing
AU - Cheng, Xiao Jie
AU - Park, Hyun Jin
AU - Chen, Xi Guang
PY - 2013/3/1
Y1 - 2013/3/1
N2 - Oleoyl-carboxymethyl-chitosan (OCMCS) was synthesized and were soluble at neutral pH. The critical micelle concentration (CMC) of OCMCS in deionized water was 0.021. mg/ml. OCMCS nanoparticles were successfully prepared via self-assembly with mean diameter of 215.34. nm, zeta potential of 19.26. mV and an almost spherical shape as determined by electron microscopy. The OCMCS nanoparticles showed low erythrocyte membrane-damaging effect. The MTT survival assay indicated no significant cytotoxicity to Caco-2 cells and MEFs cells. The uptake of FITC labeled OCMCS nanoparticles by Caco-2 cells was confirmed via confocal laser scanning microscope (CLSM). In vivo toxicity assays were performed via histopathological evaluation, and no specific anatomical pathological changes or tissue damage was observed in the tissues of carps. The extent of tissue distribution and retention following oral administration of FITC-OCMCS nanoparticles was analyzed for 3 days. After 3 days, the nanoparticles remained detectable in the muscle, heart, kidney, liver, intestine, and spleen. The results showed that 34.32% of the particles were localized in the liver, 18.79% in the kidney, and 17.36% in the heart. The lowest percentage was observed in the muscle. These results implied that OCMCS nanoparticles had great potential to be applied as safe carriers for the oral administration of protein drugs.
AB - Oleoyl-carboxymethyl-chitosan (OCMCS) was synthesized and were soluble at neutral pH. The critical micelle concentration (CMC) of OCMCS in deionized water was 0.021. mg/ml. OCMCS nanoparticles were successfully prepared via self-assembly with mean diameter of 215.34. nm, zeta potential of 19.26. mV and an almost spherical shape as determined by electron microscopy. The OCMCS nanoparticles showed low erythrocyte membrane-damaging effect. The MTT survival assay indicated no significant cytotoxicity to Caco-2 cells and MEFs cells. The uptake of FITC labeled OCMCS nanoparticles by Caco-2 cells was confirmed via confocal laser scanning microscope (CLSM). In vivo toxicity assays were performed via histopathological evaluation, and no specific anatomical pathological changes or tissue damage was observed in the tissues of carps. The extent of tissue distribution and retention following oral administration of FITC-OCMCS nanoparticles was analyzed for 3 days. After 3 days, the nanoparticles remained detectable in the muscle, heart, kidney, liver, intestine, and spleen. The results showed that 34.32% of the particles were localized in the liver, 18.79% in the kidney, and 17.36% in the heart. The lowest percentage was observed in the muscle. These results implied that OCMCS nanoparticles had great potential to be applied as safe carriers for the oral administration of protein drugs.
KW - Biocompatibility
KW - Biodistribution
KW - Cellular uptake
KW - Nanoparticles
KW - OCMCS
UR - http://www.scopus.com/inward/record.url?scp=84870788289&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84870788289&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2012.11.012
DO - 10.1016/j.colsurfb.2012.11.012
M3 - Article
VL - 103
SP - 345
EP - 353
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
ER -