TY - JOUR
T1 - Biocontrol activity of volatile-producing Bacillus megaterium and Pseudomonas protegens against Aspergillus flavus and Aflatoxin production on stored rice grains
AU - Mannaa, Mohamed
AU - Oh, Ji Yeon
AU - Kim, Ki Deok
N1 - Funding Information:
This work was supported by a Korea University Grant. We thank anonymous reviewers for valuable comments on this manuscript.
Publisher Copyright:
© The Korean Society of Mycology.
PY - 2017
Y1 - 2017
N2 - In our previous study, three bacterial strains, Bacillus megaterium KU143, Microbacterium testaceum KU313, and Pseudomonas protegens AS15, were selected as effective biocontrol agents against Aspergillus flavus on stored rice grains. In this study, we evaluated the inhibitory effects of the volatiles produced by the strains on A. flavus growth and aflatoxin production on stored rice grains. The three strains significantly reduced mycelial growth of A. flavus in dual-culture assays compared with the negative control strain, Sphingomonas aquatilis KU408, and an untreated control. Of these tested strains, volatiles produced by B. megaterium KU143 and P. protegens AS15 markedly inhibited mycelial growth, sporulation, and conidial germination of A. flavus on agar medium and suppressed the fungal populations in rice grains. Moreover, volatiles produced by these two strains significantly reduced aflatoxin production in the rice grains by A. flavus. To our knowledge, this is the first report of the suppression of A. flavus aflatoxin production in rice grains using B. megaterium and P. protegens volatiles.
AB - In our previous study, three bacterial strains, Bacillus megaterium KU143, Microbacterium testaceum KU313, and Pseudomonas protegens AS15, were selected as effective biocontrol agents against Aspergillus flavus on stored rice grains. In this study, we evaluated the inhibitory effects of the volatiles produced by the strains on A. flavus growth and aflatoxin production on stored rice grains. The three strains significantly reduced mycelial growth of A. flavus in dual-culture assays compared with the negative control strain, Sphingomonas aquatilis KU408, and an untreated control. Of these tested strains, volatiles produced by B. megaterium KU143 and P. protegens AS15 markedly inhibited mycelial growth, sporulation, and conidial germination of A. flavus on agar medium and suppressed the fungal populations in rice grains. Moreover, volatiles produced by these two strains significantly reduced aflatoxin production in the rice grains by A. flavus. To our knowledge, this is the first report of the suppression of A. flavus aflatoxin production in rice grains using B. megaterium and P. protegens volatiles.
KW - Aflatoxin
KW - Aspergillus flavus
KW - Bacillus megaterium
KW - Bacterial volatiles
KW - Pseudomonas protegens
UR - http://www.scopus.com/inward/record.url?scp=85032791320&partnerID=8YFLogxK
U2 - 10.5941/MYCO.2017.45.3.213
DO - 10.5941/MYCO.2017.45.3.213
M3 - Article
AN - SCOPUS:85032791320
VL - 45
SP - 213
EP - 219
JO - Mycobiology
JF - Mycobiology
SN - 1229-8093
IS - 3
ER -