Bioconversion of linoleic acid to conjugated linoleic acid by Bifidobacterium breve

Yoon Seok Song, Seong Woo Kang, Deok Kun Oh, Yong Taik Rho, Suk In Hong, Seung Wook Kim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The bioconversion of linoleic acid (LA) to conjugated linoleic acid (CLA) was investigated to examine LA-adaptation of Bifidobacterium breve KCTC 3461 to additions of 1 to 5 mg/mL of LA overtime. To induce LA-adaptation, B. breve KCTC 3461 was treated with LA, according to three schemes. For LA-adapted B. breve the maximum concentration of CLA, 300-350 μg/mL, was obtained in cys-MRS medium containing 1 mg/mL of LA. The CLA production significantly increased with increasing LA concentration, from 1 to 4 mg/mL, but the conversion of LA to CLA gradually decreased. The CLA production capability of B. breve, and its tolerance, improved significantly with LA-adaptation. The addition of LA (1 mg/mL) into the culture broth after 24 h of cultivation in a 100-mL media bottle was most effective at promoting CLA production. In a 2.5-L stirred-tank bioreactor, the observed conversion and productivity of 56.6% and 35.4 μgmL-1h-1, respectively, by LA-adapted B. breve were approximately 6.6 and 9.8 times higher than those of LA-unadapted B. breve.

Original languageEnglish
Pages (from-to)357-361
Number of pages5
JournalBiotechnology and Bioprocess Engineering
Volume10
Issue number4
DOIs
Publication statusPublished - 2005 Jan 1

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Keywords

  • Bifidobacterium breve
  • Bioconversion
  • Conjugated linoleic acid (CLA)
  • Linoleic acid (LA)
  • Linoleic acid-adaptation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

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