Biodegradation of 2,4,5-trichlorophenol using cell-free culture broths of Phanerochaete chrysosporium

S. H. Choi, S. H. Moon, J. S. Lee, Man Bock Gu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Cell-free culture broth of Phanerochaete chrysosporium has been adopted to biologically degrade 2,4,5-trichlorophenol. Two different medium compositions of nitrogen-sufficient and nitrogen-limited were compared for their distribution of isozymes, activity of lignin peroxidase, and production of oxalate. The two different culture broths were tested for their ability to degrade 2,4,5-trichlorophenol, and the biodegradation efficiency was estimated in terms of the disappearance of 2,4,5-trichlorophenol. The degradation efficiency for the nitrogen-limited culture broth was higher than that of the nitrogen-sufficient culture broth, since the nitrogen-limited culture broth induced lignin peroxidases (LiPs) and manganese peroxidases (MnPs), and contained sufficient oxalate for producing necessary radicals. Finally, the possible mechanism of 2,4,5-CP degradation using the nitrogen-limited culture broth was proposed.

Original languageEnglish
Pages (from-to)759-763
Number of pages5
JournalJournal of Microbiology and Biotechnology
Volume10
Issue number6
Publication statusPublished - 2000 Dec 1
Externally publishedYes

Fingerprint

Phanerochaete
Biodegradation
Nitrogen
Cell Culture Techniques
Oxalates
manganese peroxidase
Lignin
Degradation
Isoenzymes
Manganese
2,4,5-trichlorophenol
Chemical analysis

Keywords

  • 2,4,5-trichlorophenol
  • Cell-free culture broth
  • Lignin peroxidase
  • Oxalate
  • Phanerochaete chrysosporium

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Biodegradation of 2,4,5-trichlorophenol using cell-free culture broths of Phanerochaete chrysosporium. / Choi, S. H.; Moon, S. H.; Lee, J. S.; Gu, Man Bock.

In: Journal of Microbiology and Biotechnology, Vol. 10, No. 6, 01.12.2000, p. 759-763.

Research output: Contribution to journalArticle

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