Abstract
Cell-free culture broth of Phanerochaete chrysosporium has been adopted to biologically degrade 2,4,5-trichlorophenol. Two different medium compositions of nitrogen-sufficient and nitrogen-limited were compared for their distribution of isozymes, activity of lignin peroxidase, and production of oxalate. The two different culture broths were tested for their ability to degrade 2,4,5-trichlorophenol, and the biodegradation efficiency was estimated in terms of the disappearance of 2,4,5-trichlorophenol. The degradation efficiency for the nitrogen-limited culture broth was higher than that of the nitrogen-sufficient culture broth, since the nitrogen-limited culture broth induced lignin peroxidases (LiPs) and manganese peroxidases (MnPs), and contained sufficient oxalate for producing necessary radicals. Finally, the possible mechanism of 2,4,5-CP degradation using the nitrogen-limited culture broth was proposed.
| Original language | English |
|---|---|
| Pages (from-to) | 759-763 |
| Number of pages | 5 |
| Journal | Journal of Microbiology and Biotechnology |
| Volume | 10 |
| Issue number | 6 |
| Publication status | Published - 2000 Dec 1 |
| Externally published | Yes |
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Keywords
- 2,4,5-trichlorophenol
- Cell-free culture broth
- Lignin peroxidase
- Oxalate
- Phanerochaete chrysosporium
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
- Microbiology
Cite this
Biodegradation of 2,4,5-trichlorophenol using cell-free culture broths of Phanerochaete chrysosporium. / Choi, S. H.; Moon, S. H.; Lee, J. S.; Gu, Man Bock.
In: Journal of Microbiology and Biotechnology, Vol. 10, No. 6, 01.12.2000, p. 759-763.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biodegradation of 2,4,5-trichlorophenol using cell-free culture broths of Phanerochaete chrysosporium
AU - Choi, S. H.
AU - Moon, S. H.
AU - Lee, J. S.
AU - Gu, Man Bock
PY - 2000/12/1
Y1 - 2000/12/1
N2 - Cell-free culture broth of Phanerochaete chrysosporium has been adopted to biologically degrade 2,4,5-trichlorophenol. Two different medium compositions of nitrogen-sufficient and nitrogen-limited were compared for their distribution of isozymes, activity of lignin peroxidase, and production of oxalate. The two different culture broths were tested for their ability to degrade 2,4,5-trichlorophenol, and the biodegradation efficiency was estimated in terms of the disappearance of 2,4,5-trichlorophenol. The degradation efficiency for the nitrogen-limited culture broth was higher than that of the nitrogen-sufficient culture broth, since the nitrogen-limited culture broth induced lignin peroxidases (LiPs) and manganese peroxidases (MnPs), and contained sufficient oxalate for producing necessary radicals. Finally, the possible mechanism of 2,4,5-CP degradation using the nitrogen-limited culture broth was proposed.
AB - Cell-free culture broth of Phanerochaete chrysosporium has been adopted to biologically degrade 2,4,5-trichlorophenol. Two different medium compositions of nitrogen-sufficient and nitrogen-limited were compared for their distribution of isozymes, activity of lignin peroxidase, and production of oxalate. The two different culture broths were tested for their ability to degrade 2,4,5-trichlorophenol, and the biodegradation efficiency was estimated in terms of the disappearance of 2,4,5-trichlorophenol. The degradation efficiency for the nitrogen-limited culture broth was higher than that of the nitrogen-sufficient culture broth, since the nitrogen-limited culture broth induced lignin peroxidases (LiPs) and manganese peroxidases (MnPs), and contained sufficient oxalate for producing necessary radicals. Finally, the possible mechanism of 2,4,5-CP degradation using the nitrogen-limited culture broth was proposed.
KW - 2,4,5-trichlorophenol
KW - Cell-free culture broth
KW - Lignin peroxidase
KW - Oxalate
KW - Phanerochaete chrysosporium
UR - http://www.scopus.com/inward/record.url?scp=0034527204&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034527204&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0034527204
VL - 10
SP - 759
EP - 763
JO - Journal of Microbiology and Biotechnology
JF - Journal of Microbiology and Biotechnology
SN - 1017-7825
IS - 6
ER -