Abstract
In this study, various factors, such as temperature, pressure, agitation speed, water content, and the concentration and ratio of immobilized ROL and CRL were investigated for the efficient enzymatic production of biodiesel using a supercritical carbon dioxide process. Furthermore, a stepwise reaction method for the maintenance of immobilized lipase activity was optimized. Optimal conditions for biodiesel production were determined to be as follows: 130. bar pressure, 45 °C temperature, 250. rpm agitation speed, 10% water content, and 20% immobilized ROL and CRL (1:1). When batch process was performed under optimal conditions, the biodiesel conversion yield was 99.13% at 3. h. Biodiesel conversion yield was 99.99% at 2. h when 90. mmol methanol was used in a stepwise reaction. Moreover, the conversion yield of biodiesel produced by the repeated recycling of immobilized lipase in the stepwise reactions was 85% after 20 reuses.
| Original language | English |
|---|---|
| Pages (from-to) | 2105-2108 |
| Number of pages | 4 |
| Journal | Bioresource Technology |
| Volume | 102 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2011 Jan 1 |
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Keywords
- Biodiesel
- Initial reaction rate
- Lipase
- Optimization
- Supercritical carbon dioxide process
ASJC Scopus subject areas
- Bioengineering
- Environmental Engineering
- Waste Management and Disposal
Cite this
Biodiesel production by a mixture of Candida rugosa and Rhizopus oryzae lipases using a supercritical carbon dioxide process. / Lee, Jong Ho; Kim, Sung Bong; Kang, Seong Woo; Song, Yoon Seok; Park, Chulhwan; Han, Sung Ok; Kim, Seung Wook.
In: Bioresource Technology, Vol. 102, No. 2, 01.01.2011, p. 2105-2108.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biodiesel production by a mixture of Candida rugosa and Rhizopus oryzae lipases using a supercritical carbon dioxide process
AU - Lee, Jong Ho
AU - Kim, Sung Bong
AU - Kang, Seong Woo
AU - Song, Yoon Seok
AU - Park, Chulhwan
AU - Han, Sung Ok
AU - Kim, Seung Wook
PY - 2011/1/1
Y1 - 2011/1/1
N2 - In this study, various factors, such as temperature, pressure, agitation speed, water content, and the concentration and ratio of immobilized ROL and CRL were investigated for the efficient enzymatic production of biodiesel using a supercritical carbon dioxide process. Furthermore, a stepwise reaction method for the maintenance of immobilized lipase activity was optimized. Optimal conditions for biodiesel production were determined to be as follows: 130. bar pressure, 45 °C temperature, 250. rpm agitation speed, 10% water content, and 20% immobilized ROL and CRL (1:1). When batch process was performed under optimal conditions, the biodiesel conversion yield was 99.13% at 3. h. Biodiesel conversion yield was 99.99% at 2. h when 90. mmol methanol was used in a stepwise reaction. Moreover, the conversion yield of biodiesel produced by the repeated recycling of immobilized lipase in the stepwise reactions was 85% after 20 reuses.
AB - In this study, various factors, such as temperature, pressure, agitation speed, water content, and the concentration and ratio of immobilized ROL and CRL were investigated for the efficient enzymatic production of biodiesel using a supercritical carbon dioxide process. Furthermore, a stepwise reaction method for the maintenance of immobilized lipase activity was optimized. Optimal conditions for biodiesel production were determined to be as follows: 130. bar pressure, 45 °C temperature, 250. rpm agitation speed, 10% water content, and 20% immobilized ROL and CRL (1:1). When batch process was performed under optimal conditions, the biodiesel conversion yield was 99.13% at 3. h. Biodiesel conversion yield was 99.99% at 2. h when 90. mmol methanol was used in a stepwise reaction. Moreover, the conversion yield of biodiesel produced by the repeated recycling of immobilized lipase in the stepwise reactions was 85% after 20 reuses.
KW - Biodiesel
KW - Initial reaction rate
KW - Lipase
KW - Optimization
KW - Supercritical carbon dioxide process
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UR - http://www.scopus.com/inward/citedby.url?scp=78650702257&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2010.08.034
DO - 10.1016/j.biortech.2010.08.034
M3 - Article
C2 - 20813518
AN - SCOPUS:78650702257
VL - 102
SP - 2105
EP - 2108
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
IS - 2
ER -