Biodiesel production by a mixture of Candida rugosa and Rhizopus oryzae lipases using a supercritical carbon dioxide process

Jong Ho Lee, Sung Bong Kim, Seong Woo Kang, Yoon Seok Song, Chulhwan Park, Sung Ok Han, Seung Wook Kim

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

In this study, various factors, such as temperature, pressure, agitation speed, water content, and the concentration and ratio of immobilized ROL and CRL were investigated for the efficient enzymatic production of biodiesel using a supercritical carbon dioxide process. Furthermore, a stepwise reaction method for the maintenance of immobilized lipase activity was optimized. Optimal conditions for biodiesel production were determined to be as follows: 130. bar pressure, 45 °C temperature, 250. rpm agitation speed, 10% water content, and 20% immobilized ROL and CRL (1:1). When batch process was performed under optimal conditions, the biodiesel conversion yield was 99.13% at 3. h. Biodiesel conversion yield was 99.99% at 2. h when 90. mmol methanol was used in a stepwise reaction. Moreover, the conversion yield of biodiesel produced by the repeated recycling of immobilized lipase in the stepwise reactions was 85% after 20 reuses.

Original languageEnglish
Pages (from-to)2105-2108
Number of pages4
JournalBioresource Technology
Volume102
Issue number2
DOIs
Publication statusPublished - 2011 Jan 1

Fingerprint

Carbon dioxide process
Biofuels
Candida
Lipases
Biodiesel
Lipase
carbon dioxide
water content
Water content
methanol
recycling
temperature
Methanol
Recycling
Temperature
speed

Keywords

  • Biodiesel
  • Initial reaction rate
  • Lipase
  • Optimization
  • Supercritical carbon dioxide process

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

Biodiesel production by a mixture of Candida rugosa and Rhizopus oryzae lipases using a supercritical carbon dioxide process. / Lee, Jong Ho; Kim, Sung Bong; Kang, Seong Woo; Song, Yoon Seok; Park, Chulhwan; Han, Sung Ok; Kim, Seung Wook.

In: Bioresource Technology, Vol. 102, No. 2, 01.01.2011, p. 2105-2108.

Research output: Contribution to journalArticle

Lee, Jong Ho ; Kim, Sung Bong ; Kang, Seong Woo ; Song, Yoon Seok ; Park, Chulhwan ; Han, Sung Ok ; Kim, Seung Wook. / Biodiesel production by a mixture of Candida rugosa and Rhizopus oryzae lipases using a supercritical carbon dioxide process. In: Bioresource Technology. 2011 ; Vol. 102, No. 2. pp. 2105-2108.
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