Biodiesel production by enzymatic process using Jatropha oil and waste soybean oil

Ja Hyun Lee, Sung Bong Kim, Hah Young Yoo, Young Joon Suh, Gyung Bo Kang, Woo In Jang, Jongwon Kang, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In this study, non-edible Jatropha oil and postcooking waste soybean oil were utilized for enzymatic biodiesel production. The process was optimized by using a statistical method. In addition, a novel continuous process using co-immobilized Rhizopus oryzae and Candida rugosa lipases was developed. The optimum conditions for the batch process were determined to be a reaction temperature of 45oC, an agitation speed of 250 rpm, 10 wt% of water, and 20% of immobilized lipases. A conversion of about 98% at 4 h could be achieved for biodiesel production using Jatropha oil, while a conversion of about 97% at 4 h was achieved from waste soybean oil. A packed bed reactor charged with co-immobilized lipases was employed for continuous biodiesel production from Jatropha and waste soybean oil. The reactor consisted of a jacketed glass column (ID 25 mm × 130 mm), in which a temperature of 45 C was maintained by water circulation. A maximum conversion of about 80% in 24 h at a flow rate of 0.8 mL/ min was achieved with the continuous process, whereas in the two-stage continuous process, a conversion of about 90% in 72 h was attained at a flow rate of 0.1 mL/min.

Original languageEnglish
Pages (from-to)703-708
Number of pages6
JournalBiotechnology and Bioprocess Engineering
Volume18
Issue number4
DOIs
Publication statusPublished - 2013 Jul 1

Fingerprint

Jatropha
Soybean oil
Soybean Oil
Biofuels
Lipases
Biodiesel
Lipase
Oils
Flow rate
Rhizopus
Temperature
Water
Candida
Packed beds
Glass
Statistical methods

Keywords

  • biodiesel
  • continuous process
  • lipase
  • optimization
  • response surface methodology

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering
  • Bioengineering

Cite this

Biodiesel production by enzymatic process using Jatropha oil and waste soybean oil. / Lee, Ja Hyun; Kim, Sung Bong; Yoo, Hah Young; Suh, Young Joon; Kang, Gyung Bo; Jang, Woo In; Kang, Jongwon; Park, Chulhwan; Kim, Seung Wook.

In: Biotechnology and Bioprocess Engineering, Vol. 18, No. 4, 01.07.2013, p. 703-708.

Research output: Contribution to journalArticle

Lee, Ja Hyun ; Kim, Sung Bong ; Yoo, Hah Young ; Suh, Young Joon ; Kang, Gyung Bo ; Jang, Woo In ; Kang, Jongwon ; Park, Chulhwan ; Kim, Seung Wook. / Biodiesel production by enzymatic process using Jatropha oil and waste soybean oil. In: Biotechnology and Bioprocess Engineering. 2013 ; Vol. 18, No. 4. pp. 703-708.
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