Biodiesel production using a mixture of immobilized Rhizopus oryzae and Candida rugosa lipases

Dong Hwan Lee, Jung Mo Kim, Hyun Yong Shin, Seong Woo Kang, Seung Wook Kim

Research output: Contribution to journalArticle

68 Citations (Scopus)


Biodiesel conversion from soybean oil reached a maximum of 70% at 18 h using immobilized 1,3-specific Rhizopus oryzae lipase alone. Biodiesel conversion failed to reach 20% after 30 h when immobilized nonspecific Candida rugosa lipase alone was used. To increase the biodiesel production yield, a mixture of immobilized 1,3-specific R. oryzae lipase and nonspecific C. rugosa lipase was used. Using this mixture a conversion of greater than 99% at 21 h was attained. When the stability of the immobilized lipases mixture was tested, biodiesel conversion was maintained at over 80% of its original conversion after 10 cycles.

Original languageEnglish
Pages (from-to)522-525
Number of pages4
JournalBiotechnology and Bioprocess Engineering
Issue number6
Publication statusPublished - 2006 Nov 1



  • Biodiesel
  • Candida rugosa lipase
  • Immobilization
  • Reuse
  • Rhizopus oryzae lipase

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

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