Biofilm reduction potential of micro-plasma discharged water (m-PDW) against the microbes isolated from a tofu manufacturing plant

Mi Hyun Kim, Muhammad Saiful Islam Khan, Kwang Won Lee, Yun Ji Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In this study, biofilm inactivation potential of micro-plasma discharged water (m-PDW), a commercially available water purifier known as EP purification, was evaluated. Bacterial strains were collected from the various contaminated area of tofu manufacturing industry, overall 15 different types of bacterial strains were isolated and their biofilm formation ability was determined by using crystal violet (CV) staining. Three different strains (20% of the isolated strains), namely, Brevundimonas naejangsanensis, Delfita lacustris and Lactococcus lactis, with biofilm density (OD590) > 0.4, were identified as biofilm-producing strains. These three biofilm-producing strains were used to form biofilm on stainless steel (SS) coupons in a laboratory condition for four days and the biofilm containing coupons were treated with EP purification device. The inactivation obtained after 40 min of treatment was 4.8 and 4.2 log CFU/coupon for Brevundimonas naejangsanensis and Delfita lacustris, respectively, whereas no viable count was detected for Lactococcus lactis after the same treatment time. Biofilm reduction effects of m-PDW treatment differ according to biofilm-producing ability or strain characteristics. SEI (Secondary Electron Images) was obtained by field emission scanning electron microscope (FE-SEM) that showed m-PDW treatment has significant reduction potential on biofilm inactivation for the sample collected from tofu manufacturing industry.

Original languageEnglish
Pages (from-to)719-724
Number of pages6
JournalLWT
Volume97
DOIs
Publication statusPublished - 2018 Nov 1

Fingerprint

Soy Foods
tofu
Biofilms
factories
biofilm
microorganisms
Water
water
Brevundimonas
inactivation
Lactococcus lactis
Water Purification
water treatment
manufacturing
Manufacturing and Industrial Facilities
Electrons
industry
Gentian Violet
gentian violet
Stainless Steel

Keywords

  • Biofilm
  • EP purification
  • m-PDW
  • Stainless steel (SS) coupon
  • Tofu

ASJC Scopus subject areas

  • Food Science

Cite this

Biofilm reduction potential of micro-plasma discharged water (m-PDW) against the microbes isolated from a tofu manufacturing plant. / Kim, Mi Hyun; Khan, Muhammad Saiful Islam; Lee, Kwang Won; Kim, Yun Ji.

In: LWT, Vol. 97, 01.11.2018, p. 719-724.

Research output: Contribution to journalArticle

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