Biolighted Nanotorch Capable of Systemic Self-Delivery and Diagnostic Imaging

Ajay Singh, Young Hun Seo, Chang Keun Lim, Joonseok Koh, Woo Dong Jang, Ick Chan Kwon, Sehoon Kim

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Sensitive imaging of inflammation with a background-free chemiluminescence (CL) signal has great potential as a clinically relevant way of early diagnosis for various inflammatory diseases. However, to date, its feasibility has been limitedly demonstrated in vivo with locally induced inflammation models by in situ injection of CL probes. To enable systemic disease targeting and imaging by intravenous administration of CL probes, hurdles need to be overcome such as weak CL emission, short glowing duration, or inability of long blood circulation. Here, we report a CL nanoprobe (BioNT) that surmounted such limitations to perform precise identification of inflammation by systemic self-delivery to the pathological tissues. This BioNT probe was engineered by physical nanointegration of multiple kinds of functional molecules into the ultrafine nanoreactor structure (∼15 nm in size) that combines solid-state fluorescence-induced enhanced peroxalate CL and built-in machinery to control the intraparticle kinetics of CL reaction. Upon intravenous injection into a normal mouse, BioNT showed facile blood circulation and generated a self-lighted strong CL torchlight throughout the whole body owing to the tiny colloidal structure with an antifouling surface as well as high CL sensitivity toward endogenous biological hydrogen peroxide (H2O2). In mouse models of local and systemic inflammations, blood-injected BioNT visualized precise locations of inflamed tissues with dual selectivity (selective probe accumulation and selective CL reaction with H2O2 overproduced by inflammation). Even a tumor model that demands a long blood circulation time for targeting (>3 h) could be accurately identified by persistent signaling from the kinetics-tailored BioNT with a 65-fold slowed CL decay rate. We also show that BioNT exhibits no apparent toxicity, thus holding potential for high-contrast diagnostic imaging.

Original languageEnglish
Pages (from-to)9906-9911
Number of pages6
JournalACS Nano
Volume9
Issue number10
DOIs
Publication statusPublished - 2015 Oct 27
Externally publishedYes

Fingerprint

Chemiluminescence
chemiluminescence
delivery
Imaging techniques
blood circulation
Hemodynamics
probes
mice
Nanoreactors
Tissue
injection
Nanoprobes
antifouling
Kinetics
machinery
kinetics
hydrogen peroxide
Hydrogen peroxide
toxicity
Hydrogen Peroxide

Keywords

  • chemiluminescence
  • diagnosis
  • in vivo imaging
  • nanoprobes
  • systemic delivery

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

Singh, A., Seo, Y. H., Lim, C. K., Koh, J., Jang, W. D., Kwon, I. C., & Kim, S. (2015). Biolighted Nanotorch Capable of Systemic Self-Delivery and Diagnostic Imaging. ACS Nano, 9(10), 9906-9911. https://doi.org/10.1021/acsnano.5b03377

Biolighted Nanotorch Capable of Systemic Self-Delivery and Diagnostic Imaging. / Singh, Ajay; Seo, Young Hun; Lim, Chang Keun; Koh, Joonseok; Jang, Woo Dong; Kwon, Ick Chan; Kim, Sehoon.

In: ACS Nano, Vol. 9, No. 10, 27.10.2015, p. 9906-9911.

Research output: Contribution to journalArticle

Singh, A, Seo, YH, Lim, CK, Koh, J, Jang, WD, Kwon, IC & Kim, S 2015, 'Biolighted Nanotorch Capable of Systemic Self-Delivery and Diagnostic Imaging', ACS Nano, vol. 9, no. 10, pp. 9906-9911. https://doi.org/10.1021/acsnano.5b03377
Singh A, Seo YH, Lim CK, Koh J, Jang WD, Kwon IC et al. Biolighted Nanotorch Capable of Systemic Self-Delivery and Diagnostic Imaging. ACS Nano. 2015 Oct 27;9(10):9906-9911. https://doi.org/10.1021/acsnano.5b03377
Singh, Ajay ; Seo, Young Hun ; Lim, Chang Keun ; Koh, Joonseok ; Jang, Woo Dong ; Kwon, Ick Chan ; Kim, Sehoon. / Biolighted Nanotorch Capable of Systemic Self-Delivery and Diagnostic Imaging. In: ACS Nano. 2015 ; Vol. 9, No. 10. pp. 9906-9911.
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