Biological and genetic characteristics of Glyptotendipes tokunagai (Diptera

Chironomidae) on the basis of successive rearing of forty-two generations over seven years under laboratory conditions

Min Jeong Baek, Tae Joong Yoon, Hyo Jeong Kang, Yeon Jae Bae

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Members of the nonbiting midge family Chironomidae have been used worldwide as water-quality indicators or toxicity test organisms. The purpose of this study was to establish the chironomid Glyptotendipes tokunagai Sasa as a new test species by conducting successive rearing under laboratory conditions. We monitored biological and genetic aspects of >42 successive generations over 7 yr, and also compared the development of the 39th generation with the fourth generation under five constant temperatures of 15, 20, 25, 30, and 35°C. We observed that the number of eggs in an egg mass and the adult body sizes decreased rapidly in the early generations, and thereafter tended to stabilize from the fifth generation to the 42nd generation. In all generations, the mean hatching rate was >75%. Males were predominant in the early generations, but the sex ratio increased to 0.5 (ranged 0.240.61) in later generations. The genetic divergence of the reared generations, analyzed by using the mitochondrial cytochrome c oxidase subunit I gene, decreased from 0.0049 to 0.0004 as the generations progressed. In comparison with the fourth generation, the mortality and developmental time of the 39th generation were generally greater, and the adult body sizes were generally smaller. The estimated low developmental threshold temperatures of eggs, male larvae to male adults, and female larvae to female adults were 9.6, 11.3, and 9.7°C, respectively. The optimal rearing temperature was determined to be 25°C. This is the first record of domesticated rearing of a wild chironomid species under laboratory conditions for >7 yr.

Original languageEnglish
Pages (from-to)1406-1418
Number of pages13
JournalEnvironmental Entomology
Volume43
Issue number5
DOIs
Publication statusPublished - 2014

Fingerprint

Chironomidae
rearing
egg
body size
Sasa
larva
temperature
larvae
toxicity testing
toxicity test
egg masses
cytochrome-c oxidase
sex ratio
cytochrome
hatching
water quality
divergence
mortality
genetic variation
Glyptotendipes tokunagai

Keywords

  • Chironomidae
  • Glyptotendipes tokunagai
  • Optimal rearing temperature
  • Successive rearing
  • Toxicity test species

ASJC Scopus subject areas

  • Insect Science
  • Ecology, Evolution, Behavior and Systematics
  • Ecology

Cite this

@article{1f96a5995c5b49e3b327800b9d4469aa,
title = "Biological and genetic characteristics of Glyptotendipes tokunagai (Diptera: Chironomidae) on the basis of successive rearing of forty-two generations over seven years under laboratory conditions",
abstract = "Members of the nonbiting midge family Chironomidae have been used worldwide as water-quality indicators or toxicity test organisms. The purpose of this study was to establish the chironomid Glyptotendipes tokunagai Sasa as a new test species by conducting successive rearing under laboratory conditions. We monitored biological and genetic aspects of >42 successive generations over 7 yr, and also compared the development of the 39th generation with the fourth generation under five constant temperatures of 15, 20, 25, 30, and 35°C. We observed that the number of eggs in an egg mass and the adult body sizes decreased rapidly in the early generations, and thereafter tended to stabilize from the fifth generation to the 42nd generation. In all generations, the mean hatching rate was >75{\%}. Males were predominant in the early generations, but the sex ratio increased to 0.5 (ranged 0.240.61) in later generations. The genetic divergence of the reared generations, analyzed by using the mitochondrial cytochrome c oxidase subunit I gene, decreased from 0.0049 to 0.0004 as the generations progressed. In comparison with the fourth generation, the mortality and developmental time of the 39th generation were generally greater, and the adult body sizes were generally smaller. The estimated low developmental threshold temperatures of eggs, male larvae to male adults, and female larvae to female adults were 9.6, 11.3, and 9.7°C, respectively. The optimal rearing temperature was determined to be 25°C. This is the first record of domesticated rearing of a wild chironomid species under laboratory conditions for >7 yr.",
keywords = "Chironomidae, Glyptotendipes tokunagai, Optimal rearing temperature, Successive rearing, Toxicity test species",
author = "Baek, {Min Jeong} and Yoon, {Tae Joong} and Kang, {Hyo Jeong} and Bae, {Yeon Jae}",
year = "2014",
doi = "10.1603/EN14052",
language = "English",
volume = "43",
pages = "1406--1418",
journal = "Environmental Entomology",
issn = "0046-225X",
publisher = "Entomological Society of America",
number = "5",

}

TY - JOUR

T1 - Biological and genetic characteristics of Glyptotendipes tokunagai (Diptera

T2 - Chironomidae) on the basis of successive rearing of forty-two generations over seven years under laboratory conditions

AU - Baek, Min Jeong

AU - Yoon, Tae Joong

AU - Kang, Hyo Jeong

AU - Bae, Yeon Jae

PY - 2014

Y1 - 2014

N2 - Members of the nonbiting midge family Chironomidae have been used worldwide as water-quality indicators or toxicity test organisms. The purpose of this study was to establish the chironomid Glyptotendipes tokunagai Sasa as a new test species by conducting successive rearing under laboratory conditions. We monitored biological and genetic aspects of >42 successive generations over 7 yr, and also compared the development of the 39th generation with the fourth generation under five constant temperatures of 15, 20, 25, 30, and 35°C. We observed that the number of eggs in an egg mass and the adult body sizes decreased rapidly in the early generations, and thereafter tended to stabilize from the fifth generation to the 42nd generation. In all generations, the mean hatching rate was >75%. Males were predominant in the early generations, but the sex ratio increased to 0.5 (ranged 0.240.61) in later generations. The genetic divergence of the reared generations, analyzed by using the mitochondrial cytochrome c oxidase subunit I gene, decreased from 0.0049 to 0.0004 as the generations progressed. In comparison with the fourth generation, the mortality and developmental time of the 39th generation were generally greater, and the adult body sizes were generally smaller. The estimated low developmental threshold temperatures of eggs, male larvae to male adults, and female larvae to female adults were 9.6, 11.3, and 9.7°C, respectively. The optimal rearing temperature was determined to be 25°C. This is the first record of domesticated rearing of a wild chironomid species under laboratory conditions for >7 yr.

AB - Members of the nonbiting midge family Chironomidae have been used worldwide as water-quality indicators or toxicity test organisms. The purpose of this study was to establish the chironomid Glyptotendipes tokunagai Sasa as a new test species by conducting successive rearing under laboratory conditions. We monitored biological and genetic aspects of >42 successive generations over 7 yr, and also compared the development of the 39th generation with the fourth generation under five constant temperatures of 15, 20, 25, 30, and 35°C. We observed that the number of eggs in an egg mass and the adult body sizes decreased rapidly in the early generations, and thereafter tended to stabilize from the fifth generation to the 42nd generation. In all generations, the mean hatching rate was >75%. Males were predominant in the early generations, but the sex ratio increased to 0.5 (ranged 0.240.61) in later generations. The genetic divergence of the reared generations, analyzed by using the mitochondrial cytochrome c oxidase subunit I gene, decreased from 0.0049 to 0.0004 as the generations progressed. In comparison with the fourth generation, the mortality and developmental time of the 39th generation were generally greater, and the adult body sizes were generally smaller. The estimated low developmental threshold temperatures of eggs, male larvae to male adults, and female larvae to female adults were 9.6, 11.3, and 9.7°C, respectively. The optimal rearing temperature was determined to be 25°C. This is the first record of domesticated rearing of a wild chironomid species under laboratory conditions for >7 yr.

KW - Chironomidae

KW - Glyptotendipes tokunagai

KW - Optimal rearing temperature

KW - Successive rearing

KW - Toxicity test species

UR - http://www.scopus.com/inward/record.url?scp=84923093096&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84923093096&partnerID=8YFLogxK

U2 - 10.1603/EN14052

DO - 10.1603/EN14052

M3 - Article

VL - 43

SP - 1406

EP - 1418

JO - Environmental Entomology

JF - Environmental Entomology

SN - 0046-225X

IS - 5

ER -