Biological imaging of HEK293 cells expressing PLCγ1 using surface-enhanced raman microscopy

Sangyeop Lee, Sungyong Kim, Jaebum Choo, Young Shin Soon, Young Han Lee, Young Choi Ha, Seunghan Ha, Kyungho Kang, Chil Hwan Oh

Research output: Contribution to journalArticle

228 Citations (Scopus)

Abstract

Surface-enhanced Raman scattering (SERS) imaging has been used for the targeting and imaging of specific cancer markers in live cells. For this purpose, Au/Ag core-shell nanoparticles, conjugated with monoclonal antibodies, were prepared. The procedures to label live cells with those bimetallic nanoprobes have been developed and used for highly sensitive SERS imaging of live cells. In the present study, live HEK293 cells expressing PLCγ1 have been used as the optical imaging target. Our results demonstrate the potential feasibility of SERS imaging technology for the highly sensitive imaging of cancer biomarkers in live cells.

Original languageEnglish
Pages (from-to)916-922
Number of pages7
JournalAnalytical Chemistry
Volume79
Issue number3
DOIs
Publication statusPublished - 2007 Feb 1

Fingerprint

Microscopic examination
Cells
Imaging techniques
Raman scattering
Nanoprobes
Tumor Biomarkers
Labels
Monoclonal Antibodies
Nanoparticles

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Lee, S., Kim, S., Choo, J., Soon, Y. S., Lee, Y. H., Ha, Y. C., ... Oh, C. H. (2007). Biological imaging of HEK293 cells expressing PLCγ1 using surface-enhanced raman microscopy. Analytical Chemistry, 79(3), 916-922. https://doi.org/10.1021/ac061246a

Biological imaging of HEK293 cells expressing PLCγ1 using surface-enhanced raman microscopy. / Lee, Sangyeop; Kim, Sungyong; Choo, Jaebum; Soon, Young Shin; Lee, Young Han; Ha, Young Choi; Ha, Seunghan; Kang, Kyungho; Oh, Chil Hwan.

In: Analytical Chemistry, Vol. 79, No. 3, 01.02.2007, p. 916-922.

Research output: Contribution to journalArticle

Lee, S, Kim, S, Choo, J, Soon, YS, Lee, YH, Ha, YC, Ha, S, Kang, K & Oh, CH 2007, 'Biological imaging of HEK293 cells expressing PLCγ1 using surface-enhanced raman microscopy', Analytical Chemistry, vol. 79, no. 3, pp. 916-922. https://doi.org/10.1021/ac061246a
Lee, Sangyeop ; Kim, Sungyong ; Choo, Jaebum ; Soon, Young Shin ; Lee, Young Han ; Ha, Young Choi ; Ha, Seunghan ; Kang, Kyungho ; Oh, Chil Hwan. / Biological imaging of HEK293 cells expressing PLCγ1 using surface-enhanced raman microscopy. In: Analytical Chemistry. 2007 ; Vol. 79, No. 3. pp. 916-922.
@article{40e80ef375af407a96504b3cd39fb2f4,
title = "Biological imaging of HEK293 cells expressing PLCγ1 using surface-enhanced raman microscopy",
abstract = "Surface-enhanced Raman scattering (SERS) imaging has been used for the targeting and imaging of specific cancer markers in live cells. For this purpose, Au/Ag core-shell nanoparticles, conjugated with monoclonal antibodies, were prepared. The procedures to label live cells with those bimetallic nanoprobes have been developed and used for highly sensitive SERS imaging of live cells. In the present study, live HEK293 cells expressing PLCγ1 have been used as the optical imaging target. Our results demonstrate the potential feasibility of SERS imaging technology for the highly sensitive imaging of cancer biomarkers in live cells.",
author = "Sangyeop Lee and Sungyong Kim and Jaebum Choo and Soon, {Young Shin} and Lee, {Young Han} and Ha, {Young Choi} and Seunghan Ha and Kyungho Kang and Oh, {Chil Hwan}",
year = "2007",
month = "2",
day = "1",
doi = "10.1021/ac061246a",
language = "English",
volume = "79",
pages = "916--922",
journal = "Industrial And Engineering Chemistry Analytical Edition",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "3",

}

TY - JOUR

T1 - Biological imaging of HEK293 cells expressing PLCγ1 using surface-enhanced raman microscopy

AU - Lee, Sangyeop

AU - Kim, Sungyong

AU - Choo, Jaebum

AU - Soon, Young Shin

AU - Lee, Young Han

AU - Ha, Young Choi

AU - Ha, Seunghan

AU - Kang, Kyungho

AU - Oh, Chil Hwan

PY - 2007/2/1

Y1 - 2007/2/1

N2 - Surface-enhanced Raman scattering (SERS) imaging has been used for the targeting and imaging of specific cancer markers in live cells. For this purpose, Au/Ag core-shell nanoparticles, conjugated with monoclonal antibodies, were prepared. The procedures to label live cells with those bimetallic nanoprobes have been developed and used for highly sensitive SERS imaging of live cells. In the present study, live HEK293 cells expressing PLCγ1 have been used as the optical imaging target. Our results demonstrate the potential feasibility of SERS imaging technology for the highly sensitive imaging of cancer biomarkers in live cells.

AB - Surface-enhanced Raman scattering (SERS) imaging has been used for the targeting and imaging of specific cancer markers in live cells. For this purpose, Au/Ag core-shell nanoparticles, conjugated with monoclonal antibodies, were prepared. The procedures to label live cells with those bimetallic nanoprobes have been developed and used for highly sensitive SERS imaging of live cells. In the present study, live HEK293 cells expressing PLCγ1 have been used as the optical imaging target. Our results demonstrate the potential feasibility of SERS imaging technology for the highly sensitive imaging of cancer biomarkers in live cells.

UR - http://www.scopus.com/inward/record.url?scp=33846976709&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846976709&partnerID=8YFLogxK

U2 - 10.1021/ac061246a

DO - 10.1021/ac061246a

M3 - Article

VL - 79

SP - 916

EP - 922

JO - Industrial And Engineering Chemistry Analytical Edition

JF - Industrial And Engineering Chemistry Analytical Edition

SN - 0003-2700

IS - 3

ER -