Biological imaging of HEK293 cells expressing PLCγ1 using surface-enhanced raman microscopy

Sangyeop Lee; Sungyong Kim; Jaebum Choo; Young Shin Soon; Young Han Lee; Young Choi Ha; Seunghan Ha; Kyungho Kang; Chil Hwan Oh

doi:10.1021/ac061246a

Biological imaging of HEK293 cells expressing PLCγ1 using surface-enhanced raman microscopy

Sangyeop Lee, Sungyong Kim, Jaebum Choo, Young Shin Soon, Young Han Lee, Young Choi Ha, Seunghan Ha, Kyungho Kang, Chil Hwan Oh

Research output: Contribution to journal › Article › peer-review

264 Citations (Scopus)

Abstract

Surface-enhanced Raman scattering (SERS) imaging has been used for the targeting and imaging of specific cancer markers in live cells. For this purpose, Au/Ag core-shell nanoparticles, conjugated with monoclonal antibodies, were prepared. The procedures to label live cells with those bimetallic nanoprobes have been developed and used for highly sensitive SERS imaging of live cells. In the present study, live HEK293 cells expressing PLCγ1 have been used as the optical imaging target. Our results demonstrate the potential feasibility of SERS imaging technology for the highly sensitive imaging of cancer biomarkers in live cells.

Original language	English
Pages (from-to)	916-922
Number of pages	7
Journal	Analytical Chemistry
Volume	79
Issue number	3
DOIs	https://doi.org/10.1021/ac061246a
Publication status	Published - 2007 Feb 1

ASJC Scopus subject areas

Analytical Chemistry

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/ac061246a

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abstract = "Surface-enhanced Raman scattering (SERS) imaging has been used for the targeting and imaging of specific cancer markers in live cells. For this purpose, Au/Ag core-shell nanoparticles, conjugated with monoclonal antibodies, were prepared. The procedures to label live cells with those bimetallic nanoprobes have been developed and used for highly sensitive SERS imaging of live cells. In the present study, live HEK293 cells expressing PLCγ1 have been used as the optical imaging target. Our results demonstrate the potential feasibility of SERS imaging technology for the highly sensitive imaging of cancer biomarkers in live cells.",

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AU - Lee, Sangyeop

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AU - Lee, Young Han

AU - Ha, Young Choi

AU - Ha, Seunghan

AU - Kang, Kyungho

AU - Oh, Chil Hwan

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N2 - Surface-enhanced Raman scattering (SERS) imaging has been used for the targeting and imaging of specific cancer markers in live cells. For this purpose, Au/Ag core-shell nanoparticles, conjugated with monoclonal antibodies, were prepared. The procedures to label live cells with those bimetallic nanoprobes have been developed and used for highly sensitive SERS imaging of live cells. In the present study, live HEK293 cells expressing PLCγ1 have been used as the optical imaging target. Our results demonstrate the potential feasibility of SERS imaging technology for the highly sensitive imaging of cancer biomarkers in live cells.

AB - Surface-enhanced Raman scattering (SERS) imaging has been used for the targeting and imaging of specific cancer markers in live cells. For this purpose, Au/Ag core-shell nanoparticles, conjugated with monoclonal antibodies, were prepared. The procedures to label live cells with those bimetallic nanoprobes have been developed and used for highly sensitive SERS imaging of live cells. In the present study, live HEK293 cells expressing PLCγ1 have been used as the optical imaging target. Our results demonstrate the potential feasibility of SERS imaging technology for the highly sensitive imaging of cancer biomarkers in live cells.

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Biological imaging of HEK293 cells expressing PLCγ1 using surface-enhanced raman microscopy

Abstract

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