Biological responses in osteoblast-like cell line according to thin layer hydroxyapatite coatings on anodized titanium

S. H. Sohn, H. K. Jun, C. S. Kim, K. N. Kim, S. M. Chung, Sang-Wan Shin, Jae Jun Ryu, Meyoung-Kon Kim

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Several features of the implant surface, such as roughness, topography and composition play a relevant role in implant integration with bone. This study was conducted in order to determine the effects of various thin layer hydroxyapatite (HA) coatings on anodized Ti surfaces on the biological responses of a human osteoblast-like cell line (MG63). MG63 cells were cultured on 100 nm HA (100 nm HA coating on anodized surface), 500-700 nm HA (500-700 nm HA coating on anodized surface), 1 μm HA (1 μm HA coating on anodized surface) and anodize (non-HA coating on anodized surface) Ti. The morphology of these cells was assessed by scanning electron microscopy (SEM). The cDNAs prepared from the total RNAs of the MG63 were hybridized into a human cDNA microarray (1152 elements). The appearances of the surfaces observed by SEM were different on each of the four dental substrate types. MG63 cells cultured on 100 nm HA, 1 μm HA and anodize exhibited cell-matrix interactions. It was 500-700 nm HA surface showing cell-cell interaction. In the expression of genes involved in osseointegration, several genes, including bone morphogenetic protein 2, latent transforming growth factor beta binding protein 1, catenin (cadherin-associated protein), integrin, PDGFRB and GDF-1 growth differentiation factor 1 were up-regulated on the different surfaces. Several genes, including fibroblast growth factor receptor 3, fibroblast growth factor 12 and CD4 were down-regulated on the different surfaces. The attachment and expression of key osteogenic regulatory genes were enhanced by the surface morphology of the dental materials used.

Original languageEnglish
Pages (from-to)898-911
Number of pages14
JournalJournal of Oral Rehabilitation
Volume33
Issue number12
DOIs
Publication statusPublished - 2006 Dec 1

Fingerprint

Durapatite
Titanium
Osteoblasts
Cell Line
Growth Differentiation Factor 1
Cell Communication
Electron Scanning Microscopy
Cultured Cells
Receptor, Fibroblast Growth Factor, Type 3
Platelet-Derived Growth Factor beta Receptor
Osseointegration
Dental Materials
Catenins
Bone Morphogenetic Protein 2
Fibroblast Growth Factors
Cadherins
Regulator Genes
Oligonucleotide Array Sequence Analysis
Integrins
Transforming Growth Factor beta

Keywords

  • Anodization
  • cDNA microarray
  • Expression profiling
  • Gene expression
  • Hydroxyapatite coating
  • Titanium

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Biological responses in osteoblast-like cell line according to thin layer hydroxyapatite coatings on anodized titanium. / Sohn, S. H.; Jun, H. K.; Kim, C. S.; Kim, K. N.; Chung, S. M.; Shin, Sang-Wan; Ryu, Jae Jun; Kim, Meyoung-Kon.

In: Journal of Oral Rehabilitation, Vol. 33, No. 12, 01.12.2006, p. 898-911.

Research output: Contribution to journalArticle

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