Bioluminescence reporter gene-based detection of microRNAs

Hae Young Ko, Young Sik Lee, Soonhag Kim

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

MicroRNAs (miRNAs) are an abundant class of small noncoding RNA molecules that inhibit the expression of cognate genes in multicellular organisms. These small RNAs have been demonstrated to play crucial roles in a variety of biological processes including cell differentiation, proliferation, and survival. Knowledge of specific expression patterns of miRNAs is critical for functional studies. Here, we describe a bioluminescence reporter gene-based method to measure miRNA activity in cultured cells and mice using a Gaussia luciferase reporter gene controlled by miRNA binding sites in its 3′untranslated region. This method can be used to noninvasively monitor the expression patterns of functionally active miRNAs involved in different biological processes or diseases in mice.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages85-95
Number of pages11
Volume1098
DOIs
Publication statusPublished - 2014 Jan 10

Publication series

NameMethods in Molecular Biology
Volume1098
ISSN (Print)10643745

Keywords

  • Bioluminescence reporter gene
  • Gaussia luciferase
  • Imaging
  • MicroRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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