Carbon dioxide sequestration activity was compared and evaluated using bovine carbonic anhydrase (BCA), and a water soluble protein extract derived from hemocytes from diseased shell (HDS). Para-nitrophenyl acetate (p-NPA) was used to measure the reaction rate. The kcat/Km values obtained from the Lineweaver-Burk and Michaelis-Menten equations were 230.7M-1s-1 for BCA and 194.1M-s- for HDS. Without a biocatalyst, CaCO3 production took 15 seconds on average, while it took 5 seconds on average when BCA or HDS were present, indicating an approximately 3-fold enhancement of CaCO3 production rate by the biocatalysts. The biocatalytic hydration of CO2 and its precipitation as CaCO3 in the presence of biocatalysts were investigated.
- Bovine Carbonic Anhydrase
- Carbon Dioxide
- Hemocytes from Diseased Shell
ASJC Scopus subject areas
- Chemical Engineering(all)