Biomimetic sequestration of carbon dioxide using an enzyme extracted from oyster shell

Dae Hoon Kim; Mari Vinoba; Woo Sup Shin; Kyong Soo Lim; Soon Kwan Jeong; Sung Hyun Kim

doi:10.1007/s11814-011-0071-z

Biomimetic sequestration of carbon dioxide using an enzyme extracted from oyster shell

Dae Hoon Kim, Mari Vinoba, Woo Sup Shin, Kyong Soo Lim, Soon Kwan Jeong, Sung Hyun Kim

* Honorary professors

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Carbon dioxide sequestration activity was compared and evaluated using bovine carbonic anhydrase (BCA), and a water soluble protein extract derived from hemocytes from diseased shell (HDS). Para-nitrophenyl acetate (p-NPA) was used to measure the reaction rate. The k_cat/K_m values obtained from the Lineweaver-Burk and Michaelis-Menten equations were 230.7M^-1s^-1 for BCA and 194.1M^-s^- for HDS. Without a biocatalyst, CaCO₃ production took 15 seconds on average, while it took 5 seconds on average when BCA or HDS were present, indicating an approximately 3-fold enhancement of CaCO₃ production rate by the biocatalysts. The biocatalytic hydration of CO₂ and its precipitation as CaCO₃ in the presence of biocatalysts were investigated.

Original language	English
Pages (from-to)	2081-2085
Number of pages	5
Journal	Korean Journal of Chemical Engineering
Volume	28
Issue number	10
DOIs	https://doi.org/10.1007/s11814-011-0071-z
Publication status	Published - 2011 Oct

Keywords

Biomineralization
Bovine Carbonic Anhydrase
Carbon Dioxide
Hemocytes from Diseased Shell
Sequestration

ASJC Scopus subject areas

Chemistry(all)
Chemical Engineering(all)

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title = "Biomimetic sequestration of carbon dioxide using an enzyme extracted from oyster shell",

abstract = "Carbon dioxide sequestration activity was compared and evaluated using bovine carbonic anhydrase (BCA), and a water soluble protein extract derived from hemocytes from diseased shell (HDS). Para-nitrophenyl acetate (p-NPA) was used to measure the reaction rate. The kcat/Km values obtained from the Lineweaver-Burk and Michaelis-Menten equations were 230.7M-1s-1 for BCA and 194.1M-s- for HDS. Without a biocatalyst, CaCO3 production took 15 seconds on average, while it took 5 seconds on average when BCA or HDS were present, indicating an approximately 3-fold enhancement of CaCO3 production rate by the biocatalysts. The biocatalytic hydration of CO2 and its precipitation as CaCO3 in the presence of biocatalysts were investigated.",

keywords = "Biomineralization, Bovine Carbonic Anhydrase, Carbon Dioxide, Hemocytes from Diseased Shell, Sequestration",

author = "Kim, {Dae Hoon} and Mari Vinoba and Shin, {Woo Sup} and Lim, {Kyong Soo} and Jeong, {Soon Kwan} and Kim, {Sung Hyun}",

note = "Funding Information: This research was supported by a grant (CK3-101-1-0-0) from Carbon Dioxide Reduction & Sequestration Research Center, one of the 21st Century Frontier Programs funded by the Ministry of Education Science and Technology of the Korean government.",

year = "2011",

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doi = "10.1007/s11814-011-0071-z",

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AU - Vinoba, Mari

AU - Shin, Woo Sup

AU - Lim, Kyong Soo

AU - Jeong, Soon Kwan

AU - Kim, Sung Hyun

N1 - Funding Information: This research was supported by a grant (CK3-101-1-0-0) from Carbon Dioxide Reduction & Sequestration Research Center, one of the 21st Century Frontier Programs funded by the Ministry of Education Science and Technology of the Korean government.

PY - 2011/10

Y1 - 2011/10

N2 - Carbon dioxide sequestration activity was compared and evaluated using bovine carbonic anhydrase (BCA), and a water soluble protein extract derived from hemocytes from diseased shell (HDS). Para-nitrophenyl acetate (p-NPA) was used to measure the reaction rate. The kcat/Km values obtained from the Lineweaver-Burk and Michaelis-Menten equations were 230.7M-1s-1 for BCA and 194.1M-s- for HDS. Without a biocatalyst, CaCO3 production took 15 seconds on average, while it took 5 seconds on average when BCA or HDS were present, indicating an approximately 3-fold enhancement of CaCO3 production rate by the biocatalysts. The biocatalytic hydration of CO2 and its precipitation as CaCO3 in the presence of biocatalysts were investigated.

AB - Carbon dioxide sequestration activity was compared and evaluated using bovine carbonic anhydrase (BCA), and a water soluble protein extract derived from hemocytes from diseased shell (HDS). Para-nitrophenyl acetate (p-NPA) was used to measure the reaction rate. The kcat/Km values obtained from the Lineweaver-Burk and Michaelis-Menten equations were 230.7M-1s-1 for BCA and 194.1M-s- for HDS. Without a biocatalyst, CaCO3 production took 15 seconds on average, while it took 5 seconds on average when BCA or HDS were present, indicating an approximately 3-fold enhancement of CaCO3 production rate by the biocatalysts. The biocatalytic hydration of CO2 and its precipitation as CaCO3 in the presence of biocatalysts were investigated.

KW - Biomineralization

KW - Bovine Carbonic Anhydrase

KW - Carbon Dioxide

KW - Hemocytes from Diseased Shell

KW - Sequestration

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Biomimetic sequestration of carbon dioxide using an enzyme extracted from oyster shell

Abstract

Keywords

ASJC Scopus subject areas

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