Biomimetic sequestration of carbon dioxide using an enzyme extracted from oyster shell

Dae Hoon Kim, Mari Vinoba, Woo Sup Shin, Kyong Soo Lim, Soon Kwan Jeong, Sung Hyun Kim

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


Carbon dioxide sequestration activity was compared and evaluated using bovine carbonic anhydrase (BCA), and a water soluble protein extract derived from hemocytes from diseased shell (HDS). Para-nitrophenyl acetate (p-NPA) was used to measure the reaction rate. The kcat/Km values obtained from the Lineweaver-Burk and Michaelis-Menten equations were 230.7M-1s-1 for BCA and 194.1M-s- for HDS. Without a biocatalyst, CaCO3 production took 15 seconds on average, while it took 5 seconds on average when BCA or HDS were present, indicating an approximately 3-fold enhancement of CaCO3 production rate by the biocatalysts. The biocatalytic hydration of CO2 and its precipitation as CaCO3 in the presence of biocatalysts were investigated.

Original languageEnglish
Pages (from-to)2081-2085
Number of pages5
JournalKorean Journal of Chemical Engineering
Issue number10
Publication statusPublished - 2011 Oct


  • Biomineralization
  • Bovine Carbonic Anhydrase
  • Carbon Dioxide
  • Hemocytes from Diseased Shell
  • Sequestration

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)


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