Biomimetic virus-based colourimetric sensors

Jin Woo Oh, Woo Jae Chung, Kwang Heo, Hyo Eon Jin, Byung Yang Lee, Eddie Wang, Chris Zueger, Winnie Wong, Joel Meyer, Chuntae Kim, So Young Lee, Won Geun Kim, Marcin Zemla, Manfred Auer, Alexander Hexemer, Seung Wuk Lee

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

Many materials in nature change colours in response to stimuli, making them attractive for use as sensor platform. However, both natural materials and their synthetic analogues lack selectivity towards specific chemicals, and introducing such selectivity remains a challenge. Here we report the self-assembly of genetically engineered viruses (M13 phage) into target-specific, colourimetric biosensors. The sensors are composed of phage-bundle nanostructures and exhibit viewing-angle independent colour, similar to collagen structures in turkey skin. On exposure to various volatile organic chemicals, the structures rapidly swell and undergo distinct colour changes. Furthermore, sensors composed of phage displaying trinitrotoluene (TNT)-binding peptide motifs identified from a phage display selectively distinguish TNT down to 300 p.p.b. over similarly structured chemicals. Our tunable, colourimetric sensors can be useful for the detection of a variety of harmful toxicants and pathogens to protect human health and national security.

Original languageEnglish
Article number3043
JournalNature Communications
Volume5
DOIs
Publication statusPublished - 2014 Jan 21

Fingerprint

Biomimetics
Bacteriophages
biomimetics
viruses
Viruses
Trinitrotoluene
Color
trinitrotoluene
sensors
Sensors
color
Security Measures
Bacteriophage M13
Organic Chemicals
Nanostructures
selectivity
Biosensing Techniques
Self Report
pathogens
National security

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Oh, J. W., Chung, W. J., Heo, K., Jin, H. E., Lee, B. Y., Wang, E., ... Lee, S. W. (2014). Biomimetic virus-based colourimetric sensors. Nature Communications, 5, [3043]. https://doi.org/10.1038/ncomms4043

Biomimetic virus-based colourimetric sensors. / Oh, Jin Woo; Chung, Woo Jae; Heo, Kwang; Jin, Hyo Eon; Lee, Byung Yang; Wang, Eddie; Zueger, Chris; Wong, Winnie; Meyer, Joel; Kim, Chuntae; Lee, So Young; Kim, Won Geun; Zemla, Marcin; Auer, Manfred; Hexemer, Alexander; Lee, Seung Wuk.

In: Nature Communications, Vol. 5, 3043, 21.01.2014.

Research output: Contribution to journalArticle

Oh, JW, Chung, WJ, Heo, K, Jin, HE, Lee, BY, Wang, E, Zueger, C, Wong, W, Meyer, J, Kim, C, Lee, SY, Kim, WG, Zemla, M, Auer, M, Hexemer, A & Lee, SW 2014, 'Biomimetic virus-based colourimetric sensors', Nature Communications, vol. 5, 3043. https://doi.org/10.1038/ncomms4043
Oh, Jin Woo ; Chung, Woo Jae ; Heo, Kwang ; Jin, Hyo Eon ; Lee, Byung Yang ; Wang, Eddie ; Zueger, Chris ; Wong, Winnie ; Meyer, Joel ; Kim, Chuntae ; Lee, So Young ; Kim, Won Geun ; Zemla, Marcin ; Auer, Manfred ; Hexemer, Alexander ; Lee, Seung Wuk. / Biomimetic virus-based colourimetric sensors. In: Nature Communications. 2014 ; Vol. 5.
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