Biomineralized matrix-assisted osteogenic differentiation of human embryonic stem cells

Heemin Kang, Cai Wen, Yongsung Hwang, Yu Ru V Shih, Mrityunjoy Kar, Sung Wook Seo, Shyni Varghese

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The physical and chemical properties of a matrix play an important role in determining various cellular behaviors, including lineage specificity. We demonstrate that the differentiation commitment of human embryonic stem cells (hESCs), both in vitro and in vivo, can be solely achieved through synthetic biomaterials. hESCs cultured using mineralized synthetic matrices mimicking a calcium phosphate (CaP)-rich bone environment differentiated into osteoblasts in the absence of any osteogenic inducing supplements. When implanted in vivo, these hESC-laden mineralized matrices contributed to ectopic bone tissue formation. In contrast, cells within the corresponding non-mineralized matrices underwent either osteogenic or adipogenic fate depending upon the local cues present in the microenvironment. To our knowledge, this is the first demonstration where synthetic matrices are shown to induce terminal cell fate specification of hESCs exclusively by biomaterial-based cues both in vitro and in vivo. Technologies that utilize tissue specific cell-matrix interactions to control stem cell fate could be a powerful tool in regenerative medicine. Such approaches can be used as a tool to advance our basic understanding and assess the translational potential of stem cells. This journal is

Original languageEnglish
Pages (from-to)5676-5688
Number of pages13
JournalJournal of Materials Chemistry B
Volume2
Issue number34
DOIs
Publication statusPublished - 2014 Sep 14
Externally publishedYes

Fingerprint

Stem cells
Biocompatible Materials
Cues
Stem Cells
Bone and Bones
Biomaterials
Choristoma
Regenerative Medicine
Bone
Osteoblasts
Osteogenesis
Cell Communication
Tissue
Calcium phosphate
Technology
Chemical properties
Human Embryonic Stem Cells
Demonstrations
Physical properties
Specifications

ASJC Scopus subject areas

  • Chemistry(all)
  • Biomedical Engineering
  • Medicine(all)
  • Materials Science(all)

Cite this

Biomineralized matrix-assisted osteogenic differentiation of human embryonic stem cells. / Kang, Heemin; Wen, Cai; Hwang, Yongsung; Shih, Yu Ru V; Kar, Mrityunjoy; Seo, Sung Wook; Varghese, Shyni.

In: Journal of Materials Chemistry B, Vol. 2, No. 34, 14.09.2014, p. 5676-5688.

Research output: Contribution to journalArticle

Kang, Heemin ; Wen, Cai ; Hwang, Yongsung ; Shih, Yu Ru V ; Kar, Mrityunjoy ; Seo, Sung Wook ; Varghese, Shyni. / Biomineralized matrix-assisted osteogenic differentiation of human embryonic stem cells. In: Journal of Materials Chemistry B. 2014 ; Vol. 2, No. 34. pp. 5676-5688.
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