Biomolecular Imaging of Regeneration of Zebrafish Caudal Fins Using High Spatial Resolution Ambient Mass Spectrometry

Jae Young Kim, Sun Young Lee, Hyunmin Kim, Ji Won Park, Dong-Kwon Lim, Dae Won Moon

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We observed the molecular distribution changes that occurred during the regeneration of fresh zebrafish caudal fins using the recently developed ambient high-resolution mass spectrometry (MS) imaging technique of atmospheric pressure-nanoparticle and plasma-assisted laser desorption ionization (AP-nanoPALDI). AP-nanoPALDI analyses of fresh zebrafish caudal fins revealed that the small molecules, including neurotransmitters, amino acids, lipids, and metabolites of the regenerated area, were more evenly distributed throughout the bony rays and inter-ray mesenchymal tissues compared to the original area in the early stage. Zebrafish caudal fins of less than 200 μm thickness can be very useful for tissue regeneration studies using ambient MS imaging by providing sufficient biomolecular information at the molecular level for wound-healing studies. AP-nanoPALDI imaging was compared with a complementary MS imaging tool, surface sensitive time-of-flight secondary ion MS (ToF-SIMS).

Original languageEnglish
Pages (from-to)12723-12730
Number of pages8
JournalAnalytical chemistry
Volume90
Issue number21
DOIs
Publication statusPublished - 2018 Nov 6

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Mass spectrometry
Atmospheric pressure
Ionization
Imaging techniques
Desorption
Nanoparticles
Plasmas
Lasers
Tissue regeneration
Secondary ion mass spectrometry
Metabolites
Neurotransmitter Agents
Tissue
Lipids
Amino Acids
Molecules

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Biomolecular Imaging of Regeneration of Zebrafish Caudal Fins Using High Spatial Resolution Ambient Mass Spectrometry. / Kim, Jae Young; Lee, Sun Young; Kim, Hyunmin; Park, Ji Won; Lim, Dong-Kwon; Moon, Dae Won.

In: Analytical chemistry, Vol. 90, No. 21, 06.11.2018, p. 12723-12730.

Research output: Contribution to journalArticle

Kim, Jae Young ; Lee, Sun Young ; Kim, Hyunmin ; Park, Ji Won ; Lim, Dong-Kwon ; Moon, Dae Won. / Biomolecular Imaging of Regeneration of Zebrafish Caudal Fins Using High Spatial Resolution Ambient Mass Spectrometry. In: Analytical chemistry. 2018 ; Vol. 90, No. 21. pp. 12723-12730.
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