Biostability and biocompatibility of a surface-grafted phospholipid monolayer on a solid substrate

Kwangmeyung Kim, Chulhee Kim, Youngro Byun

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

We have previously demonstrated phosphorylcholine monolayer chemically grafted onto a methacryloyl-terminated solid substrate by in situ polymerization. The in situ polymerization was carried out at the interface between a pre-assembled acrylated phospholipid monolayer produced by vesicle fusion and a methacryloyl-terminated substrate using a water-soluble initiator, 2,2′-azobis(2-methylpropionamidine) dihydrochloride (AAPD). Herein, we examined the biostability and biocompatibility of a surface-grafted phospholipid monolayer (poly-PC) on a methacryloyl-terminated substrate using a "wash off' test, in vitro protein adsorption and in vivo cage implantation for time intervals of 4, 7, 14 and 21 days, respectively. In order to compare the biostability and biocompatibility of phospholipid surfaces on solid substrates, we used two types of phospholipid surfaces: a physically adsorbed phospholipid monolayer (PC) and a poly-PC. Atomic force microscopy and water contact angle measurements indicated that the poly-PC surface was more stable in PBS, Triton X-100 and to EO gas sterilization than the PC surface. The adsorption of proteins such as albumin, fibrinogen, IgG and human plasma proteins on the poly-PC surfaces were significantly reduced, in vitro. Moreover, the poly-PC surface greatly reduced macrophage adhesion and the formation of foreign body giant cells, in vivo.

Original languageEnglish
Pages (from-to)33-41
Number of pages9
JournalBiomaterials
Volume25
Issue number1
DOIs
Publication statusPublished - 2004 Jan
Externally publishedYes

Keywords

  • Biocompatibility
  • Biostability
  • Cytomimetic biomaterials
  • In situ polymerization
  • Phospholipid monolayer

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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