Biosurfactant-assisted bioremediation of crude oil by indigenous bacteria isolated from Taean beach sediment

Dong Wan Lee, Hanbyul Lee, Bong Oh Kwon, Jong Seong Khim, Un Hyuk Yim, Beom Seok Kim, Jae-Jin Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Crude oil and its derivatives are considered as one group of the most pervasive environmental pollutants in marine environments. Bioremediation using oil-degrading bacteria has emerged as a promising green cleanup alternative in more recent years. The employment of biosurfactant-producing and hydrocarbon-utilizing indigenous bacteria enhances the effectiveness of bioremediation by making hydrocarbons bioavailable for degradation. In this study, the best candidates of biosurfactant-producing indigenous bacteria were selected by screening of biochemical tests. The selected bacteria include Bacillus algicola (003-Phe1), Rhodococcus soli (102-Na5), Isoptericola chiayiensis (103-Na4), and Pseudoalteromonas agarivorans (SDRB-Py1). In general, these isolated species caused low surface tension values (33.9–41.3 mN m−1), high oil spreading (1.2–2.4 cm), and hydrocarbon emulsification (up to 65%) warranting active degradation of hydrocarbons. FT-IR and LC-MS analyses indicated that the monorhamnolipid (Rha-C16:1) and dirhamnolipid (Rha-Rha-C6-C6:1) were commonly produced by the bacteria as potent biosurfactants. The residual crude oil after the biodegradation test was quantitated using GC-MS analysis. The bacteria utilized crude oil as their sole carbon source while the amount of residual crude oil significantly decreased. In addition the cell-free broth containing biosurfactants produced by bacterial strains significantly desorbed crude oil in oil-polluted marine sediment. The selected bacteria might hold additional capacity in crude oil degradation. Biosurfactant-producing indigenous bacteria therefore degrade crude oil hydrocarbon compounds, produce biosurfactants that can increase the emulsification of crude oil and are thus more conducive to the degradation of crude oil. Biosurfactant-producing indigenous bacteria can effectively expedite the bioremediation of crude oil contaminated environments.

Original languageEnglish
Pages (from-to)254-264
Number of pages11
JournalEnvironmental Pollution
Volume241
DOIs
Publication statusPublished - 2018 Oct 1

Fingerprint

Environmental Biodegradation
Bioremediation
Petroleum
Beaches
Bacteria
Sediments
Crude oil
Hydrocarbons
Degradation
Oils
Emulsification
Pseudoalteromonas
Geologic Sediments
Rhodococcus
Environmental Pollutants
Surface Tension
Bacilli
Biodegradation
Bacillus
Surface tension

Keywords

  • Biosurfactant-producing bacteria
  • Desorption
  • Microorganism
  • PAHs
  • Rhamnolipid
  • Taean oil spill

ASJC Scopus subject areas

  • Toxicology
  • Pollution
  • Health, Toxicology and Mutagenesis

Cite this

Biosurfactant-assisted bioremediation of crude oil by indigenous bacteria isolated from Taean beach sediment. / Lee, Dong Wan; Lee, Hanbyul; Kwon, Bong Oh; Khim, Jong Seong; Yim, Un Hyuk; Kim, Beom Seok; Kim, Jae-Jin.

In: Environmental Pollution, Vol. 241, 01.10.2018, p. 254-264.

Research output: Contribution to journalArticle

Lee, Dong Wan ; Lee, Hanbyul ; Kwon, Bong Oh ; Khim, Jong Seong ; Yim, Un Hyuk ; Kim, Beom Seok ; Kim, Jae-Jin. / Biosurfactant-assisted bioremediation of crude oil by indigenous bacteria isolated from Taean beach sediment. In: Environmental Pollution. 2018 ; Vol. 241. pp. 254-264.
@article{ad034fd444a74025a42671990589f25a,
title = "Biosurfactant-assisted bioremediation of crude oil by indigenous bacteria isolated from Taean beach sediment",
abstract = "Crude oil and its derivatives are considered as one group of the most pervasive environmental pollutants in marine environments. Bioremediation using oil-degrading bacteria has emerged as a promising green cleanup alternative in more recent years. The employment of biosurfactant-producing and hydrocarbon-utilizing indigenous bacteria enhances the effectiveness of bioremediation by making hydrocarbons bioavailable for degradation. In this study, the best candidates of biosurfactant-producing indigenous bacteria were selected by screening of biochemical tests. The selected bacteria include Bacillus algicola (003-Phe1), Rhodococcus soli (102-Na5), Isoptericola chiayiensis (103-Na4), and Pseudoalteromonas agarivorans (SDRB-Py1). In general, these isolated species caused low surface tension values (33.9–41.3 mN m−1), high oil spreading (1.2–2.4 cm), and hydrocarbon emulsification (up to 65{\%}) warranting active degradation of hydrocarbons. FT-IR and LC-MS analyses indicated that the monorhamnolipid (Rha-C16:1) and dirhamnolipid (Rha-Rha-C6-C6:1) were commonly produced by the bacteria as potent biosurfactants. The residual crude oil after the biodegradation test was quantitated using GC-MS analysis. The bacteria utilized crude oil as their sole carbon source while the amount of residual crude oil significantly decreased. In addition the cell-free broth containing biosurfactants produced by bacterial strains significantly desorbed crude oil in oil-polluted marine sediment. The selected bacteria might hold additional capacity in crude oil degradation. Biosurfactant-producing indigenous bacteria therefore degrade crude oil hydrocarbon compounds, produce biosurfactants that can increase the emulsification of crude oil and are thus more conducive to the degradation of crude oil. Biosurfactant-producing indigenous bacteria can effectively expedite the bioremediation of crude oil contaminated environments.",
keywords = "Biosurfactant-producing bacteria, Desorption, Microorganism, PAHs, Rhamnolipid, Taean oil spill",
author = "Lee, {Dong Wan} and Hanbyul Lee and Kwon, {Bong Oh} and Khim, {Jong Seong} and Yim, {Un Hyuk} and Kim, {Beom Seok} and Jae-Jin Kim",
year = "2018",
month = "10",
day = "1",
doi = "10.1016/j.envpol.2018.05.070",
language = "English",
volume = "241",
pages = "254--264",
journal = "Environmental Pollution",
issn = "0269-7491",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Biosurfactant-assisted bioremediation of crude oil by indigenous bacteria isolated from Taean beach sediment

AU - Lee, Dong Wan

AU - Lee, Hanbyul

AU - Kwon, Bong Oh

AU - Khim, Jong Seong

AU - Yim, Un Hyuk

AU - Kim, Beom Seok

AU - Kim, Jae-Jin

PY - 2018/10/1

Y1 - 2018/10/1

N2 - Crude oil and its derivatives are considered as one group of the most pervasive environmental pollutants in marine environments. Bioremediation using oil-degrading bacteria has emerged as a promising green cleanup alternative in more recent years. The employment of biosurfactant-producing and hydrocarbon-utilizing indigenous bacteria enhances the effectiveness of bioremediation by making hydrocarbons bioavailable for degradation. In this study, the best candidates of biosurfactant-producing indigenous bacteria were selected by screening of biochemical tests. The selected bacteria include Bacillus algicola (003-Phe1), Rhodococcus soli (102-Na5), Isoptericola chiayiensis (103-Na4), and Pseudoalteromonas agarivorans (SDRB-Py1). In general, these isolated species caused low surface tension values (33.9–41.3 mN m−1), high oil spreading (1.2–2.4 cm), and hydrocarbon emulsification (up to 65%) warranting active degradation of hydrocarbons. FT-IR and LC-MS analyses indicated that the monorhamnolipid (Rha-C16:1) and dirhamnolipid (Rha-Rha-C6-C6:1) were commonly produced by the bacteria as potent biosurfactants. The residual crude oil after the biodegradation test was quantitated using GC-MS analysis. The bacteria utilized crude oil as their sole carbon source while the amount of residual crude oil significantly decreased. In addition the cell-free broth containing biosurfactants produced by bacterial strains significantly desorbed crude oil in oil-polluted marine sediment. The selected bacteria might hold additional capacity in crude oil degradation. Biosurfactant-producing indigenous bacteria therefore degrade crude oil hydrocarbon compounds, produce biosurfactants that can increase the emulsification of crude oil and are thus more conducive to the degradation of crude oil. Biosurfactant-producing indigenous bacteria can effectively expedite the bioremediation of crude oil contaminated environments.

AB - Crude oil and its derivatives are considered as one group of the most pervasive environmental pollutants in marine environments. Bioremediation using oil-degrading bacteria has emerged as a promising green cleanup alternative in more recent years. The employment of biosurfactant-producing and hydrocarbon-utilizing indigenous bacteria enhances the effectiveness of bioremediation by making hydrocarbons bioavailable for degradation. In this study, the best candidates of biosurfactant-producing indigenous bacteria were selected by screening of biochemical tests. The selected bacteria include Bacillus algicola (003-Phe1), Rhodococcus soli (102-Na5), Isoptericola chiayiensis (103-Na4), and Pseudoalteromonas agarivorans (SDRB-Py1). In general, these isolated species caused low surface tension values (33.9–41.3 mN m−1), high oil spreading (1.2–2.4 cm), and hydrocarbon emulsification (up to 65%) warranting active degradation of hydrocarbons. FT-IR and LC-MS analyses indicated that the monorhamnolipid (Rha-C16:1) and dirhamnolipid (Rha-Rha-C6-C6:1) were commonly produced by the bacteria as potent biosurfactants. The residual crude oil after the biodegradation test was quantitated using GC-MS analysis. The bacteria utilized crude oil as their sole carbon source while the amount of residual crude oil significantly decreased. In addition the cell-free broth containing biosurfactants produced by bacterial strains significantly desorbed crude oil in oil-polluted marine sediment. The selected bacteria might hold additional capacity in crude oil degradation. Biosurfactant-producing indigenous bacteria therefore degrade crude oil hydrocarbon compounds, produce biosurfactants that can increase the emulsification of crude oil and are thus more conducive to the degradation of crude oil. Biosurfactant-producing indigenous bacteria can effectively expedite the bioremediation of crude oil contaminated environments.

KW - Biosurfactant-producing bacteria

KW - Desorption

KW - Microorganism

KW - PAHs

KW - Rhamnolipid

KW - Taean oil spill

UR - http://www.scopus.com/inward/record.url?scp=85047473061&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047473061&partnerID=8YFLogxK

U2 - 10.1016/j.envpol.2018.05.070

DO - 10.1016/j.envpol.2018.05.070

M3 - Article

C2 - 29807284

AN - SCOPUS:85047473061

VL - 241

SP - 254

EP - 264

JO - Environmental Pollution

JF - Environmental Pollution

SN - 0269-7491

ER -