Biosynthesis of 2-phenylethanol from glucose with genetically engineered Kluyveromyces marxianus

Tae Yeon Kim, Sang Woo Lee, Min Kyu Oh

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

2-Phenylethanol (2-PE) is an aromatic alcohol with a rose scent, which is used in the cosmetics, fragrance and food industries. 2-PE is produced in a few yeast strains by Ehrlich pathway. In this study, Kluyveromyces marxianus was genetically engineered for overproduction of 2-PE from glucose. About 1.0g/L of 2-PE was produced by overexpressing phenylpyruvate decarboxylase (ARO10) and alcohol dehydrogenase (ADH2) genes of Saccharomyces cerevisiae. A similar level of 2-PE was also produced from evolved K. marxianus, which was resistant to the phenylalanine analog, p-fluorophenylalanine. aroG fbr from Klebsiella pneumoniae encoding a feedback resistant mutant of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DHAP) synthase was overexpressed in the evolved K. marxianus. Finally, 1.3g/L of 2-PE was produced from 20g/L glucose without addition of phenylalanine in the medium.

Original languageEnglish
Pages (from-to)44-47
Number of pages4
JournalEnzyme and Microbial Technology
Volume61-62
DOIs
Publication statusPublished - 2014 Jul

Keywords

  • 2-Phenylethanol
  • DHAP synthase
  • Ehrlich pathway
  • Kluyveromyces marxianus

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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