Biosynthesis of 2-phenylethanol from glucose with genetically engineered Kluyveromyces marxianus

Tae Yeon Kim; Sang Woo Lee; Min Kyu Oh

doi:10.1016/j.enzmictec.2014.04.011

Biosynthesis of 2-phenylethanol from glucose with genetically engineered Kluyveromyces marxianus

Tae Yeon Kim, Sang Woo Lee, Min Kyu Oh

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

58 Citations (Scopus)

Abstract

2-Phenylethanol (2-PE) is an aromatic alcohol with a rose scent, which is used in the cosmetics, fragrance and food industries. 2-PE is produced in a few yeast strains by Ehrlich pathway. In this study, Kluyveromyces marxianus was genetically engineered for overproduction of 2-PE from glucose. About 1.0g/L of 2-PE was produced by overexpressing phenylpyruvate decarboxylase (ARO10) and alcohol dehydrogenase (ADH2) genes of Saccharomyces cerevisiae. A similar level of 2-PE was also produced from evolved K. marxianus, which was resistant to the phenylalanine analog, p-fluorophenylalanine. aroG ^fbr from Klebsiella pneumoniae encoding a feedback resistant mutant of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DHAP) synthase was overexpressed in the evolved K. marxianus. Finally, 1.3g/L of 2-PE was produced from 20g/L glucose without addition of phenylalanine in the medium.

Original language	English
Pages (from-to)	44-47
Number of pages	4
Journal	Enzyme and Microbial Technology
Volume	61-62
DOIs	https://doi.org/10.1016/j.enzmictec.2014.04.011
Publication status	Published - 2014 Jul

Keywords

2-Phenylethanol
DHAP synthase
Ehrlich pathway
Kluyveromyces marxianus

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

Access to Document

10.1016/j.enzmictec.2014.04.011

Cite this

@article{2c2d1c871a314f35bcbd2f2af4e1ba59,

title = "Biosynthesis of 2-phenylethanol from glucose with genetically engineered Kluyveromyces marxianus",

abstract = "2-Phenylethanol (2-PE) is an aromatic alcohol with a rose scent, which is used in the cosmetics, fragrance and food industries. 2-PE is produced in a few yeast strains by Ehrlich pathway. In this study, Kluyveromyces marxianus was genetically engineered for overproduction of 2-PE from glucose. About 1.0g/L of 2-PE was produced by overexpressing phenylpyruvate decarboxylase (ARO10) and alcohol dehydrogenase (ADH2) genes of Saccharomyces cerevisiae. A similar level of 2-PE was also produced from evolved K. marxianus, which was resistant to the phenylalanine analog, p-fluorophenylalanine. aroG fbr from Klebsiella pneumoniae encoding a feedback resistant mutant of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DHAP) synthase was overexpressed in the evolved K. marxianus. Finally, 1.3g/L of 2-PE was produced from 20g/L glucose without addition of phenylalanine in the medium.",

keywords = "2-Phenylethanol, DHAP synthase, Ehrlich pathway, Kluyveromyces marxianus",

author = "Kim, {Tae Yeon} and Lee, {Sang Woo} and Oh, {Min Kyu}",

note = "Funding Information: This research was supported by National Research Foundation of Korea (NRF) (Nos. 2011-0015358 and 2012M1A2A2026560 ). We appreciate Prof. Akada at Yamaguchi University in Japan, who constructed and donated URA-auxotroph of K. marxianus strain via the NBRP. ",

year = "2014",

month = jul,

doi = "10.1016/j.enzmictec.2014.04.011",

language = "English",

volume = "61-62",

pages = "44--47",

journal = "Enzyme and Microbial Technology",

issn = "0141-0229",

publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Biosynthesis of 2-phenylethanol from glucose with genetically engineered Kluyveromyces marxianus

AU - Kim, Tae Yeon

AU - Lee, Sang Woo

AU - Oh, Min Kyu

N1 - Funding Information: This research was supported by National Research Foundation of Korea (NRF) (Nos. 2011-0015358 and 2012M1A2A2026560 ). We appreciate Prof. Akada at Yamaguchi University in Japan, who constructed and donated URA-auxotroph of K. marxianus strain via the NBRP.

PY - 2014/7

Y1 - 2014/7

N2 - 2-Phenylethanol (2-PE) is an aromatic alcohol with a rose scent, which is used in the cosmetics, fragrance and food industries. 2-PE is produced in a few yeast strains by Ehrlich pathway. In this study, Kluyveromyces marxianus was genetically engineered for overproduction of 2-PE from glucose. About 1.0g/L of 2-PE was produced by overexpressing phenylpyruvate decarboxylase (ARO10) and alcohol dehydrogenase (ADH2) genes of Saccharomyces cerevisiae. A similar level of 2-PE was also produced from evolved K. marxianus, which was resistant to the phenylalanine analog, p-fluorophenylalanine. aroG fbr from Klebsiella pneumoniae encoding a feedback resistant mutant of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DHAP) synthase was overexpressed in the evolved K. marxianus. Finally, 1.3g/L of 2-PE was produced from 20g/L glucose without addition of phenylalanine in the medium.

AB - 2-Phenylethanol (2-PE) is an aromatic alcohol with a rose scent, which is used in the cosmetics, fragrance and food industries. 2-PE is produced in a few yeast strains by Ehrlich pathway. In this study, Kluyveromyces marxianus was genetically engineered for overproduction of 2-PE from glucose. About 1.0g/L of 2-PE was produced by overexpressing phenylpyruvate decarboxylase (ARO10) and alcohol dehydrogenase (ADH2) genes of Saccharomyces cerevisiae. A similar level of 2-PE was also produced from evolved K. marxianus, which was resistant to the phenylalanine analog, p-fluorophenylalanine. aroG fbr from Klebsiella pneumoniae encoding a feedback resistant mutant of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DHAP) synthase was overexpressed in the evolved K. marxianus. Finally, 1.3g/L of 2-PE was produced from 20g/L glucose without addition of phenylalanine in the medium.

KW - 2-Phenylethanol

KW - DHAP synthase

KW - Ehrlich pathway

KW - Kluyveromyces marxianus

UR - http://www.scopus.com/inward/record.url?scp=84900804607&partnerID=8YFLogxK

U2 - 10.1016/j.enzmictec.2014.04.011

DO - 10.1016/j.enzmictec.2014.04.011

M3 - Article

C2 - 24910335

AN - SCOPUS:84900804607

SN - 0141-0229

VL - 61-62

SP - 44

EP - 47

JO - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

ER -

Biosynthesis of 2-phenylethanol from glucose with genetically engineered Kluyveromyces marxianus

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this