Biosynthesis of glycosylated derivatives of tylosin in Streptomyces venezuelae

Ah Reum Han, Sung Ryeol Park, Je Won Park, Eun Yeol Lee, Dong Myung Kim, Byung Gee Kim, Yeo Joon Yoon

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Streptomyces venezuelae YJ028, bearing a deletion of the entire biosynthetic gene cluster encoding the pikromycin polyketide synthases and desosamine biosynthetic enzymes, was used as a bioconversion system for combinatorial biosynthesis of glycosylated derivatives of tylosin. Two engineered deoxysugar biosynthetic pathways for the biosynthesis of TDP-3-O-demethyl-D-chalcose or TDP-Lrhamnose in conjunction with the glycosyltransferaseauxiliary protein pair DesVII/DesVIII were expressed in a S. venezuelae YJ028 mutant strain. Supplementation of each mutant strain capable of producing TDP-3-O-demethyl- D-chalcose or TDP-L-rhamnose with tylosin aglycone tylactone resulted in the production of the 3-O-demethyl- D-chalcose, D-quinovose, or L-rhamnose-glycosylated tylactone.

Original languageEnglish
Pages (from-to)613-616
Number of pages4
JournalJournal of microbiology and biotechnology
Volume21
Issue number6
DOIs
Publication statusPublished - 2011 Jun 1
Externally publishedYes

Keywords

  • Combinatorial biosynthesis
  • Streptomyces venezuelae
  • Tylosin

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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