Biosynthesis of plant-specific flavones and flavonols in Streptomyces venezuelae

Sung Ryeol Park, Ji Hye Paik, Mi Sun Ahn, Je Won Park, Yeo Joon Yoon

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Recently, recombinant Streptomyces venezuelae has been established as a heterologous host for microbial production of flavanones and stilbenes, a class of plant-specific polyketides. In the present work, we expanded the applicability of the S. venezuelae system to the production of more diverse plant polyketides including flavones and flavonols. A plasmid with the synthetic codon-optimized flavone synthase I gene from Petroselium crispum was introduced to S. venezuelae DHS2001 bearing a deletion of the native pikromycin polyketide synthase gene, and the resulting strain generated flavones from exogenously fed flavanones. In addition, a recombinant S. venezuelae mutant expressing a codon-optimized flavanone 3β-hydroxylase gene from Citrus siensis and a flavonol synthase gene from Citrus unshius also successfully produced flavonols.

Original languageEnglish
Pages (from-to)1295-1299
Number of pages5
JournalJournal of microbiology and biotechnology
Volume20
Issue number9
DOIs
Publication statusPublished - 2010 Sep 1
Externally publishedYes

Keywords

  • Flavones
  • Flavonol
  • Heterologous production
  • Streptomyces venezuelae

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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