TY - JOUR
T1 - Biosynthetic Routes for Producing Various Fucosyl-Oligosaccharides
AU - Yun, Eun Ju
AU - Liu, Jing Jing
AU - Lee, Jae Won
AU - Kwak, Suryang
AU - Yu, Sora
AU - Kim, Kyoung Heon
AU - Jin, Youg Su
N1 - Funding Information:
KHK acknowledges grant support from the National Research Foundation of Korea (NRF-2017R1A2B2005628 and NRF-2018R1A4A1022589) and the facility support at the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety.
PY - 2019/2/15
Y1 - 2019/2/15
N2 - Fucosyl-oligosaccharides (FOSs) play physiologically important roles as prebiotics, neuronal growth factors, and inhibitors of enteropathogens. However, challenges in designed synthesis and mass production of FOSs hamper their industrial applications. Here, we report flexible biosynthetic routes to produce various FOSs, including unnatural ones, through in vitro enzymatic reactions of various sugar acceptors, such as glucose, cellobiose, and agarobiose, and GDP-l-fucose as the fucose donor by using α1,2-fucosyltransferase (FucT2). Also, the whole-cell conversion for fucosylation of various sugar acceptors by overexpressing the genes associated with GDP-l-fucose production and fucT2 gene in Escherichia coli was demonstrated by producing 17.74 g/L of 2′-fucosylgalactose (2′-FG). Prebiotic effects of 2′-FG were verified on the basis of selective fermentability of 2′-FG by probiotic bifidobacteria. These biosynthetic routes can be used to engineer industrial microorganisms for more economical, more flexible, and safer production of FOSs than chemical synthesis of FOSs.
AB - Fucosyl-oligosaccharides (FOSs) play physiologically important roles as prebiotics, neuronal growth factors, and inhibitors of enteropathogens. However, challenges in designed synthesis and mass production of FOSs hamper their industrial applications. Here, we report flexible biosynthetic routes to produce various FOSs, including unnatural ones, through in vitro enzymatic reactions of various sugar acceptors, such as glucose, cellobiose, and agarobiose, and GDP-l-fucose as the fucose donor by using α1,2-fucosyltransferase (FucT2). Also, the whole-cell conversion for fucosylation of various sugar acceptors by overexpressing the genes associated with GDP-l-fucose production and fucT2 gene in Escherichia coli was demonstrated by producing 17.74 g/L of 2′-fucosylgalactose (2′-FG). Prebiotic effects of 2′-FG were verified on the basis of selective fermentability of 2′-FG by probiotic bifidobacteria. These biosynthetic routes can be used to engineer industrial microorganisms for more economical, more flexible, and safer production of FOSs than chemical synthesis of FOSs.
KW - 2′-fucosylgalactose
KW - chemotherapeutics
KW - enzymatic synthesis
KW - fucosyl-oligosaccharides
KW - fucosyltransferase
KW - prebiotics
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U2 - 10.1021/acssynbio.8b00436
DO - 10.1021/acssynbio.8b00436
M3 - Article
C2 - 30668900
AN - SCOPUS:85061557950
VL - 8
SP - 415
EP - 424
JO - ACS Synthetic Biology
JF - ACS Synthetic Biology
SN - 2161-5063
IS - 2
ER -