Biotransformation of mulberroside A from Morus alba results in enhancement of tyrosinase inhibition

Jeong Keun Kim, Mijin Kim, Ssang Goo Cho, Myung Kyoo Kim, Suhng Wook Kim, Young Hee Lim

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Mulberroside A, a glycosylated stilbene, was isolated and identified from the ethanol extract of the roots of Morus alba. Oxyresveratrol, the aglycone of mulberroside A, was produced by enzymatic hydrolysis of mulberroside A using the commercial enzyme Pectinex®. Mulberroside A and oxyresveratrol showed inhibitory activity against mushroom tyrosinase with an IC50 of 53.6 and 0.49 μM, respectively. The tyrosinase inhibitory activity of oxyresveratrol was thus approximately 110-fold higher than that of mulberroside A. Inhibition kinetics showed mulberroside A to be a competitive inhibitor of mushroom tyrosinase with L-tyrosine and L-DOPA as substrate. Oxyresveratrol showed mixed inhibition and noncompetitive inhibition against L-tyrosine and L-DOPA, respectively, as substrate. The results indicate that the tyrosinase inhibitory activity of mulberroside A was greatly enhanced by the bioconversion process.

Original languageEnglish
Pages (from-to)631-637
Number of pages7
JournalJournal of Industrial Microbiology and Biotechnology
Volume37
Issue number6
DOIs
Publication statusPublished - 2010 Jun 1

Fingerprint

Morus
Monophenol Monooxygenase
Biotransformation
Agaricales
Tyrosine
Stilbenes
mulberroside A
Inhibitory Concentration 50
Hydrolysis
Ethanol
puag-haad
Enzymes

Keywords

  • Biotransformation
  • Mulberroside A
  • Oxyresveratrol
  • Tyrosinase inhibitor

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Biotransformation of mulberroside A from Morus alba results in enhancement of tyrosinase inhibition. / Kim, Jeong Keun; Kim, Mijin; Cho, Ssang Goo; Kim, Myung Kyoo; Kim, Suhng Wook; Lim, Young Hee.

In: Journal of Industrial Microbiology and Biotechnology, Vol. 37, No. 6, 01.06.2010, p. 631-637.

Research output: Contribution to journalArticle

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AU - Kim, Mijin

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AU - Kim, Suhng Wook

AU - Lim, Young Hee

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