Biphasic calcium phosphate (BCP)-immobilized porous poly (D,L-lactic-co-glycolic acid) microspheres enhance osteogenic activities of osteoblasts

Kyu Sik Shim, Sung Eun Kim, Young Pil Yun, Somang Choi, Hak Jun Kim, Kyeongsoon Park, Hae Ryong Song

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The purpose of this study was to evaluate the potential of porous poly (D,L-lactic-co-glycolic acid) (PLGA) microspheres (PMSs) immobilized on biphasic calcium phosphate nanoparticles (BCP NPs) (BCP-IM-PMSs) to enhance osteogenic activity. PMSs were fabricated using a fluidic device, and their surfaces were modified with L-lysine (aminated-PMSs), whereas the BCP NPs were modified with heparin-dopamine (Hep-DOPA) to obtain heparinized-BCP (Hep-BCP) NPs. BCP-IM-PMSs were fabricated via electrostatic interactions between the Hep-BCP NPs and aminated-PMSs. The fabricated BCP-IM-PMSs showed an interconnected pore structure. In vitro studies showed that MG-63 cells cultured on BCP-IM-PMSs had increased alkaline phosphatase activity, calcium content, and mRNA expression of osteocalcin (OCN) and osteopontin (OPN) compared with cells cultured on PMSs. These data suggest that BCP NP-immobilized PMSs have the potential to enhance osteogenic activity.

Original languageEnglish
Article number297
JournalPolymers
Volume9
Issue number7
DOIs
Publication statusPublished - 2017 Jul 21

Keywords

  • Biphasic calcium phosphate (BCP)
  • MG-63 cells
  • Osteogenic activity
  • Porous microspheres (PMSs)

ASJC Scopus subject areas

  • Chemistry(all)
  • Polymers and Plastics

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