Black rice (Oryza sativa L.) extracts induce osteoblast differentiation and protect against bone loss in ovariectomized rats

Woo Seok Jang, Cho Rong Seo, Hwan Hee Jang, No Joon Song, Jong Keun Kim, Jee Yin Ahn, Jaejoon Han, Woo Duck Seo, Young Min Lee, Kye Won Park

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Osteoporosis, an age associated skeletal disease, exhibits increased adipogenesis at the expense of osteogenesis from common osteoporotic bone marrow cells. In this study, black rice (Oryza sativa L.) extracts (BRE) were identified as osteogenic inducers. BRE stimulated the alkaline phosphatase (ALP) activity in both C3H10T1/2 and primary bone marrow cells. Similarly, BRE increased mRNA expression of ALP and osterix. Oral administration of BRE in OVX rats prevented decreases in bone density and strength. By contrast, BRE inhibited adipocyte differentiation of mesenchymal C3H10T1/2 cells and prevented increases in body weight and fat mass in high fat diet fed obese mice, further suggesting the dual effects of BRE on anti-adipogenesis and pro-osteogenesis. UPLC analysis identified cyanidin-3-O-glucoside and peonidin-3-O-glucoside as main anti-adipogenic effectors but not for pro-osteogenic induction. In mechanism studies, BRE selectively stimulated Wnt-driven luciferase activities. BRE treatment also induced Wnt-specific target genes such as Axin2, WISP2, and Cyclin D1. Taken together, these data suggest that BRE is a potentially useful ingredient to protect against age related osteoporosis and diet induced obesity. This journal is

Original languageEnglish
Pages (from-to)265-275
Number of pages11
JournalFood and Function
Volume6
Issue number1
DOIs
Publication statusPublished - 2015 Jan 1

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black rice
Adipogenesis
osteoblasts
osteoporosis
bone marrow cells
bone formation
Osteoblasts
Osteogenesis
Bone Marrow Cells
Osteoporosis
glucosides
Alkaline Phosphatase
alkaline phosphatase
Oryza sativa
bones
bone strength
Bone and Bones
Obese Mice
Cyclin D1
rats

ASJC Scopus subject areas

  • Food Science

Cite this

Jang, W. S., Seo, C. R., Jang, H. H., Song, N. J., Kim, J. K., Ahn, J. Y., ... Park, K. W. (2015). Black rice (Oryza sativa L.) extracts induce osteoblast differentiation and protect against bone loss in ovariectomized rats. Food and Function, 6(1), 265-275. https://doi.org/10.1039/c4fo00836g

Black rice (Oryza sativa L.) extracts induce osteoblast differentiation and protect against bone loss in ovariectomized rats. / Jang, Woo Seok; Seo, Cho Rong; Jang, Hwan Hee; Song, No Joon; Kim, Jong Keun; Ahn, Jee Yin; Han, Jaejoon; Seo, Woo Duck; Lee, Young Min; Park, Kye Won.

In: Food and Function, Vol. 6, No. 1, 01.01.2015, p. 265-275.

Research output: Contribution to journalArticle

Jang, WS, Seo, CR, Jang, HH, Song, NJ, Kim, JK, Ahn, JY, Han, J, Seo, WD, Lee, YM & Park, KW 2015, 'Black rice (Oryza sativa L.) extracts induce osteoblast differentiation and protect against bone loss in ovariectomized rats', Food and Function, vol. 6, no. 1, pp. 265-275. https://doi.org/10.1039/c4fo00836g
Jang, Woo Seok ; Seo, Cho Rong ; Jang, Hwan Hee ; Song, No Joon ; Kim, Jong Keun ; Ahn, Jee Yin ; Han, Jaejoon ; Seo, Woo Duck ; Lee, Young Min ; Park, Kye Won. / Black rice (Oryza sativa L.) extracts induce osteoblast differentiation and protect against bone loss in ovariectomized rats. In: Food and Function. 2015 ; Vol. 6, No. 1. pp. 265-275.
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