Blastocyst development and pregnancies after IVF of mature oocytes retrieved from unstimulated patients with PCOS after in-vivo HCG priming

Weon Young Son, San Hyun Yoon, Suk W. Lee, Yong Ko, Hye Gyun Yoon, Jin H. Lim

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

A major side-effect of controlled ovarian stimulation (COS) in patients with polycystic ovarian syndrome (PCOS) is the risk of ovarian hyperstimulation syndrome (OHSS). In-vitro maturation (IVM) of immature oocytes represents a potential alternative for the fertility treatment of these patients. Two patients at high risk of OHSS were primed with 10 000 IU HCG 36 h before oocyte retrieval. After retrieval, oocyte maturity was evaluated. Oocytes considered to be mature at the time of collection were inseminated by IVF or ICSI, and the resulting embryos were cultured to blastocysts. Transfer of these blastocysts resulted in pregnancy in both patients. Immature oocytes were cultured in YS medium supplemented with 30% human follicular fluid, 1 IU/ml rFSH, 10 IU/ml HCG and 10 ng/ml epidermal growth factor (rhEGF). After in-vitro maturation of the oocytes, ICSI was performed. Two and five expanded blastocysts were obtained after 5 day culture and were cryopreserved. This report indicates that mature oocytes can be collected at the time of retrieval using only in-vivo HCG priming in women with PCOS, and clinical pregnancy can be established by transfer of blastocysts derived from the mature oocytes. This approach opens a potential for a new dimension in the management of patients with PCOS.

Original languageEnglish
Pages (from-to)134-136
Number of pages3
JournalHuman Reproduction
Volume17
Issue number1
Publication statusPublished - 2002 Feb 18

Fingerprint

Polycystic Ovary Syndrome
Blastocyst
Oocytes
Pregnancy
In Vitro Oocyte Maturation Techniques
Ovarian Hyperstimulation Syndrome
Intracytoplasmic Sperm Injections
Embryo Transfer
Oocyte Retrieval
Follicular Fluid
Ovulation Induction
Epidermal Growth Factor
Fertility
Embryonic Structures

Keywords

  • Blastocyst
  • HCG priming
  • IVM
  • PCOS
  • Pregnancies

ASJC Scopus subject areas

  • Developmental Biology
  • Physiology
  • Obstetrics and Gynaecology
  • Reproductive Medicine

Cite this

Blastocyst development and pregnancies after IVF of mature oocytes retrieved from unstimulated patients with PCOS after in-vivo HCG priming. / Son, Weon Young; Yoon, San Hyun; Lee, Suk W.; Ko, Yong; Yoon, Hye Gyun; Lim, Jin H.

In: Human Reproduction, Vol. 17, No. 1, 18.02.2002, p. 134-136.

Research output: Contribution to journalArticle

Son, Weon Young ; Yoon, San Hyun ; Lee, Suk W. ; Ko, Yong ; Yoon, Hye Gyun ; Lim, Jin H. / Blastocyst development and pregnancies after IVF of mature oocytes retrieved from unstimulated patients with PCOS after in-vivo HCG priming. In: Human Reproduction. 2002 ; Vol. 17, No. 1. pp. 134-136.
@article{7219edb1317f47a0852492c092cb4987,
title = "Blastocyst development and pregnancies after IVF of mature oocytes retrieved from unstimulated patients with PCOS after in-vivo HCG priming",
abstract = "A major side-effect of controlled ovarian stimulation (COS) in patients with polycystic ovarian syndrome (PCOS) is the risk of ovarian hyperstimulation syndrome (OHSS). In-vitro maturation (IVM) of immature oocytes represents a potential alternative for the fertility treatment of these patients. Two patients at high risk of OHSS were primed with 10 000 IU HCG 36 h before oocyte retrieval. After retrieval, oocyte maturity was evaluated. Oocytes considered to be mature at the time of collection were inseminated by IVF or ICSI, and the resulting embryos were cultured to blastocysts. Transfer of these blastocysts resulted in pregnancy in both patients. Immature oocytes were cultured in YS medium supplemented with 30{\%} human follicular fluid, 1 IU/ml rFSH, 10 IU/ml HCG and 10 ng/ml epidermal growth factor (rhEGF). After in-vitro maturation of the oocytes, ICSI was performed. Two and five expanded blastocysts were obtained after 5 day culture and were cryopreserved. This report indicates that mature oocytes can be collected at the time of retrieval using only in-vivo HCG priming in women with PCOS, and clinical pregnancy can be established by transfer of blastocysts derived from the mature oocytes. This approach opens a potential for a new dimension in the management of patients with PCOS.",
keywords = "Blastocyst, HCG priming, IVM, PCOS, Pregnancies",
author = "Son, {Weon Young} and Yoon, {San Hyun} and Lee, {Suk W.} and Yong Ko and Yoon, {Hye Gyun} and Lim, {Jin H.}",
year = "2002",
month = "2",
day = "18",
language = "English",
volume = "17",
pages = "134--136",
journal = "Human Reproduction",
issn = "0268-1161",
publisher = "Oxford University Press",
number = "1",

}

TY - JOUR

T1 - Blastocyst development and pregnancies after IVF of mature oocytes retrieved from unstimulated patients with PCOS after in-vivo HCG priming

AU - Son, Weon Young

AU - Yoon, San Hyun

AU - Lee, Suk W.

AU - Ko, Yong

AU - Yoon, Hye Gyun

AU - Lim, Jin H.

PY - 2002/2/18

Y1 - 2002/2/18

N2 - A major side-effect of controlled ovarian stimulation (COS) in patients with polycystic ovarian syndrome (PCOS) is the risk of ovarian hyperstimulation syndrome (OHSS). In-vitro maturation (IVM) of immature oocytes represents a potential alternative for the fertility treatment of these patients. Two patients at high risk of OHSS were primed with 10 000 IU HCG 36 h before oocyte retrieval. After retrieval, oocyte maturity was evaluated. Oocytes considered to be mature at the time of collection were inseminated by IVF or ICSI, and the resulting embryos were cultured to blastocysts. Transfer of these blastocysts resulted in pregnancy in both patients. Immature oocytes were cultured in YS medium supplemented with 30% human follicular fluid, 1 IU/ml rFSH, 10 IU/ml HCG and 10 ng/ml epidermal growth factor (rhEGF). After in-vitro maturation of the oocytes, ICSI was performed. Two and five expanded blastocysts were obtained after 5 day culture and were cryopreserved. This report indicates that mature oocytes can be collected at the time of retrieval using only in-vivo HCG priming in women with PCOS, and clinical pregnancy can be established by transfer of blastocysts derived from the mature oocytes. This approach opens a potential for a new dimension in the management of patients with PCOS.

AB - A major side-effect of controlled ovarian stimulation (COS) in patients with polycystic ovarian syndrome (PCOS) is the risk of ovarian hyperstimulation syndrome (OHSS). In-vitro maturation (IVM) of immature oocytes represents a potential alternative for the fertility treatment of these patients. Two patients at high risk of OHSS were primed with 10 000 IU HCG 36 h before oocyte retrieval. After retrieval, oocyte maturity was evaluated. Oocytes considered to be mature at the time of collection were inseminated by IVF or ICSI, and the resulting embryos were cultured to blastocysts. Transfer of these blastocysts resulted in pregnancy in both patients. Immature oocytes were cultured in YS medium supplemented with 30% human follicular fluid, 1 IU/ml rFSH, 10 IU/ml HCG and 10 ng/ml epidermal growth factor (rhEGF). After in-vitro maturation of the oocytes, ICSI was performed. Two and five expanded blastocysts were obtained after 5 day culture and were cryopreserved. This report indicates that mature oocytes can be collected at the time of retrieval using only in-vivo HCG priming in women with PCOS, and clinical pregnancy can be established by transfer of blastocysts derived from the mature oocytes. This approach opens a potential for a new dimension in the management of patients with PCOS.

KW - Blastocyst

KW - HCG priming

KW - IVM

KW - PCOS

KW - Pregnancies

UR - http://www.scopus.com/inward/record.url?scp=0036161284&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036161284&partnerID=8YFLogxK

M3 - Article

VL - 17

SP - 134

EP - 136

JO - Human Reproduction

JF - Human Reproduction

SN - 0268-1161

IS - 1

ER -