Blood-pool multifunctional nanoparticles formed by temperature-induced phase transition for cancer-targeting therapy and molecular imaging

Keun Sang Oh, Sangmin Lee, Jin Hee Na, Jeong Yeon Kim, Dong Eog Kim, Kwangmeyung Kim, Ick Chan Kwon, Soon Hong Yuk, Seo Young Jeong

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Multifunctional nanoparticles (NPs) were prepared based on temperature-induced phase transition in a molten mixture of Lipiodol®, Tween 80, paclitaxel (PTX), and Pluronic F-68, wherein the Lipiodol®/Tween 80 mixture is used as a solubilizer for PTX, and Pluronic F-68 is used for the stabilization of the molten mixture. The morphology and size distribution of optimized multifunctional NPs were observed using transmittance electron microscopy (TEM) and a particle size analyzer. In the optical imaging of tumor-bearing mice using a near-infrared fluorescence (NIRF) imaging system, the multifunctional NPs were evaluated in terms of a time-dependent excretion profile, in vivo biodistribution and tumor-targeting capability compared to free fluorescence dye. In addition, the prolonged circulation of multifunctional NPs was confirmed by enhancement of the blood-pool in live animals using a micro-CT imaging system, because iodine-containing Lipiodol® has an X-ray enhancement property. Finally, the anti-tumor efficacy of multifunctional NPs was monitored by injecting the multifunctional NPs into the tail veins of tumor-bearing mice. The multifunctional NPs showed excellent tumor targetability and anti-tumor efficacy in tumor-bearing mice, caused by the enhanced permeation and retention (EPR) effect.

Original languageEnglish
Pages (from-to)192-202
Number of pages11
JournalInternational Journal of Pharmaceutics
Volume437
Issue number1-2
DOIs
Publication statusPublished - 2012 Nov 1

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Keywords

  • Cancer-targeting therapy
  • Lipiodol®
  • Molecular imaging
  • Multifunctional nanoparticles
  • Pluronic F-68
  • Temperature-induced phase transition

ASJC Scopus subject areas

  • Pharmaceutical Science

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