TY - JOUR
T1 - BLT2 phosphorylation at Thr355 by Akt is necessary for BLT2-mediated chemotaxis
AU - Wei, Jun Dong
AU - Kim, Joo Young
AU - Kim, Jae Hong
N1 - Funding Information:
This work was supported by a General Researcher Support Project ( 2011-0004241 ) and the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by Korean government (MEST) ( 2011-0027753 ). In addition, this work was supported by a grant from the Korea Healthcare Technology R&D Project, Ministry of Health & Welfare, Republic of Korea ( A101032 ).
PY - 2011/11/16
Y1 - 2011/11/16
N2 - BLT2, a low-affinity leukotriene B4 (LTB4) receptor, is a member of the G protein-coupled receptor family and is involved in multiple cellular responses, including chemotaxis. Despite its biological significance, the mechanisms of BLT2 regulation, especially by protein kinases, are poorly characterised. In this study, we found that Akt phosphorylates BLT2 at its C-terminal Thr355 residue and that this event is critical for BLT2-mediated chemotactic responses. In addition, we found that Rac1 stimulation and subsequent reactive oxygen species (ROS) production lie downstream of BLT2 phosphorylation, thus mediating chemotaxis. Structured summary of protein interactions: BLT2 physically interacts with Akt by pull down (View interaction) BLT2 physically interacts with Akt by pull down (View interaction).
AB - BLT2, a low-affinity leukotriene B4 (LTB4) receptor, is a member of the G protein-coupled receptor family and is involved in multiple cellular responses, including chemotaxis. Despite its biological significance, the mechanisms of BLT2 regulation, especially by protein kinases, are poorly characterised. In this study, we found that Akt phosphorylates BLT2 at its C-terminal Thr355 residue and that this event is critical for BLT2-mediated chemotactic responses. In addition, we found that Rac1 stimulation and subsequent reactive oxygen species (ROS) production lie downstream of BLT2 phosphorylation, thus mediating chemotaxis. Structured summary of protein interactions: BLT2 physically interacts with Akt by pull down (View interaction) BLT2 physically interacts with Akt by pull down (View interaction).
KW - Akt
KW - Chemotaxis
KW - Leukotriene B receptor 2 (BLT2)
KW - Reactive oxygen species
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U2 - 10.1016/j.febslet.2011.10.037
DO - 10.1016/j.febslet.2011.10.037
M3 - Article
C2 - 22044535
AN - SCOPUS:80855131521
VL - 585
SP - 3501
EP - 3506
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 22
ER -