BMP-2 induces motility and invasiveness by promoting colon cancer stemness through STAT3 activation

Bo Ram Kim, Sang Cheul Oh, Dae Hee Lee, Jung Lim Kim, Suk Young Lee, Myoung Hee Kang, Sun Il Lee, Sanghee Kang, Sung Yup Joung, Byung Wook Min

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Bone morphogenetic proteins (BMPs) have been involved in metastatic progression and tumorigenesis of many cancer types. However, it remains unclear how BMP-2 contributes to the initiation and development of these cancers. Here, we investigated the role of BMP-2 in colon cancer stem cell (CSC) development from colon cancer cells. We also determined the effects of BMP-2 on CSC development and epithelial-mesenchymal transition (EMT) in human colon cancer cell lines HCT-116 and SW620. We found that BMP-2 enhanced sphere formation of colon cancer cells without serum. Also, BMP-2-induced spheres displayed up-regulation of stemness markers (CD133+ and EpCAM+) and increased drug resistance, hallmarks of CSCs. Importantly, expression of EMT activators p-Smad1/5 and Snail and N-cadherin was increased in the spheres’ cells, indicating that BMP-2 signaling might result in CSC self-renewal and EMT. Furthermore, siRNA-mediated knockdown of signal transducer and activator of transcription 3 (STAT3) in HCT-116 cells reversed BMP-2-induced EMT and stem cell formation. Taken together, our results suggest that the BMP-2 induced STAT3-mediated induction of colon cancer cell metastasis requires an EMT and/or changes in CSC markers.

Original languageEnglish
Pages (from-to)9475-9486
Number of pages12
JournalTumor Biology
Volume36
Issue number12
DOIs
Publication statusPublished - 2015 Dec 1

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STAT3 Transcription Factor
Bone Morphogenetic Protein 2
Colonic Neoplasms
Transcriptional Activation
Epithelial-Mesenchymal Transition
Neoplastic Stem Cells
HCT116 Cells
Bone Morphogenetic Proteins
Cadherins
Mesenchymal Stromal Cells
Drug Resistance
Small Interfering RNA
Neoplasms
Carcinogenesis
Up-Regulation
Neoplasm Metastasis
Cell Line

Keywords

  • Bone morphogenetic proteins
  • Cancer stem cells
  • Colon cancer
  • Epithelial-mesenchymal transition
  • Signal transducer and activator of transcription 3

ASJC Scopus subject areas

  • Cancer Research

Cite this

BMP-2 induces motility and invasiveness by promoting colon cancer stemness through STAT3 activation. / Kim, Bo Ram; Oh, Sang Cheul; Lee, Dae Hee; Kim, Jung Lim; Lee, Suk Young; Kang, Myoung Hee; Lee, Sun Il; Kang, Sanghee; Joung, Sung Yup; Min, Byung Wook.

In: Tumor Biology, Vol. 36, No. 12, 01.12.2015, p. 9475-9486.

Research output: Contribution to journalArticle

Kim, Bo Ram ; Oh, Sang Cheul ; Lee, Dae Hee ; Kim, Jung Lim ; Lee, Suk Young ; Kang, Myoung Hee ; Lee, Sun Il ; Kang, Sanghee ; Joung, Sung Yup ; Min, Byung Wook. / BMP-2 induces motility and invasiveness by promoting colon cancer stemness through STAT3 activation. In: Tumor Biology. 2015 ; Vol. 36, No. 12. pp. 9475-9486.
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