BMP-2 induces the expression of chondrocyte-specific genes in bovine synovium-derived progenitor cells cultured in three-dimensional alginate hydrogel

Yongdoo Park, M. Sugimoto, A. Watrin, M. Chiquet, Ernst B. Hunziker

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

Objective: According to recent reports, the synovial membrane may contain mesenchymal stem cells with the potential to differentiate into chondrocytes under appropriate conditions. In order to assess the usefulness of synovium-derived progenitor cells for the purposes of cartilage tissue engineering, we explored their requirements for the expression of chondrocyte-specific genes after expansion in vitro. Design: Mesenchymal progenitor cells were isolated from the synovial membranes of bovine shoulder joints and expanded in two-dimensions on plastic surfaces. They were then seeded either as micromass cultures or as single cells within alginate gels, which were cultured in serum-free medium. Under these three-dimensional conditions, chondrogenesis is known to be supported and maintained. Cell cultures were exposed either to bone morphogenetic protein-2 (BMP-2) or to isoforms of transforming growth factor-β (TGF-β). The levels of mRNA for Sox9, collagen types I and II and aggrecan were determined by RT-PCR. Results: When transferred to alginate gel cultures, the fibroblast-like synovial cells assumed a rounded form. BMP-2, but not isoforms of TGF-β, stimulated, in a dose-dependent manner, the production of messenger RNAs (mRNAs) for Sox9, type II collagen and aggrecan. Under optimal conditions, the expression levels of cartilage-specific genes were comparable to those within cultured articular cartilage chondrocytes. However, in contrast to cultured articular cartilage chondrocytes, synovial cells exposed to BMP-2 continued to express the mRNA for α1(I) collagen. Conclusions: This study demonstrates that bovine synovium-derived mesenchymal progenitor cells can be induced to express chondrocyte-specific genes. However, the differentiation process is not complete under the chosen conditions. The stimulation conditions required for full transformation must now be delineated.

Original languageEnglish
Pages (from-to)527-536
Number of pages10
JournalOsteoarthritis and Cartilage
Volume13
Issue number6
DOIs
Publication statusPublished - 2005 Jun 1
Externally publishedYes

Fingerprint

Bone Morphogenetic Protein 2
Synovial Membrane
Hydrogel
Alginate
Cartilage
Chondrocytes
Hydrogels
Bone
Stem Cells
Genes
Collagen
Proteins
Mesenchymal Stromal Cells
Aggrecans
Cell culture
Articular Cartilage
Transforming Growth Factors
Gels
Messenger RNA
Membranes

Keywords

  • BMP-2
  • Chondrogenesis
  • Differentiation
  • Synoviocytes

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

BMP-2 induces the expression of chondrocyte-specific genes in bovine synovium-derived progenitor cells cultured in three-dimensional alginate hydrogel. / Park, Yongdoo; Sugimoto, M.; Watrin, A.; Chiquet, M.; Hunziker, Ernst B.

In: Osteoarthritis and Cartilage, Vol. 13, No. 6, 01.06.2005, p. 527-536.

Research output: Contribution to journalArticle

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