BnNHL18A shows a localization change by stress-inducing chemical treatments

Suk B. Lee, Byung Kook Ham, Jeong Mee Park, Young Jin Kim, Kyung Hee Paek

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The two genes, named BnNHL18A and BnNHL18B, showing sequence homology with Arabidopsis NDR1/HIN1-like (NHL) genes, were isolated from cDNA library prepared with oilseed rape (Brassica napus) seedlings treated with NaCl. The transcript level of BnNHL18A was increased by sodium chloride, ethephon, hydrogen peroxide, methyl jasmonate, or salicylic acid treatment. The coding regions of BnNHL18A and BnNHL18B contain a sarcolipin (SLN)-like sequence. Analysis of the localization of smGFP fusion proteins showed that BnNHL18A is mainly localized to endoplasmic reticulum (ER). This result suggests that the SLN-like sequence plays a role in retaining proteins in ER membrane in plants. In response to NaCl, hydrogen peroxide, ethephon, and salicylic acid treatments, the protein localization of BnNHL18A was changed. Our findings suggest a common function of BnNHL18A in biotic and abiotic stresses, and demonstrate the presence of the shared mechanism of protein translocalization between the responses to plant pathogen and to osmotic stress.

Original languageEnglish
Pages (from-to)399-406
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume339
Issue number1
DOIs
Publication statusPublished - 2006 Jan 6

Fingerprint

Salicylic Acid
Endoplasmic Reticulum
Hydrogen Peroxide
Proteins
Genes
Oilseeds
Brassica napus
Osmotic Pressure
Pathogens
Sequence Homology
Gene Library
Seedlings
Arabidopsis
Sodium Chloride
Fusion reactions
Membranes
ethephon
sarcolipin
methyl jasmonate

Keywords

  • Brassica napus
  • HIN1
  • Hypersensitive response
  • NDR1
  • Osmotic stress
  • Sarcolipin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

BnNHL18A shows a localization change by stress-inducing chemical treatments. / Lee, Suk B.; Ham, Byung Kook; Park, Jeong Mee; Kim, Young Jin; Paek, Kyung Hee.

In: Biochemical and Biophysical Research Communications, Vol. 339, No. 1, 06.01.2006, p. 399-406.

Research output: Contribution to journalArticle

Lee, Suk B. ; Ham, Byung Kook ; Park, Jeong Mee ; Kim, Young Jin ; Paek, Kyung Hee. / BnNHL18A shows a localization change by stress-inducing chemical treatments. In: Biochemical and Biophysical Research Communications. 2006 ; Vol. 339, No. 1. pp. 399-406.
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