BnNHL18A shows a localization change by stress-inducing chemical treatments

Suk Bae Lee; Byung Kook Ham; Jeong Mee Park; Young Jin Kim; Kyung Hee Paek

doi:10.1016/j.bbrc.2005.10.210

BnNHL18A shows a localization change by stress-inducing chemical treatments

Suk Bae Lee, Byung Kook Ham, Jeong Mee Park, Young Jin Kim, Kyung Hee Paek

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

The two genes, named BnNHL18A and BnNHL18B, showing sequence homology with Arabidopsis NDR1/HIN1-like (NHL) genes, were isolated from cDNA library prepared with oilseed rape (Brassica napus) seedlings treated with NaCl. The transcript level of BnNHL18A was increased by sodium chloride, ethephon, hydrogen peroxide, methyl jasmonate, or salicylic acid treatment. The coding regions of BnNHL18A and BnNHL18B contain a sarcolipin (SLN)-like sequence. Analysis of the localization of smGFP fusion proteins showed that BnNHL18A is mainly localized to endoplasmic reticulum (ER). This result suggests that the SLN-like sequence plays a role in retaining proteins in ER membrane in plants. In response to NaCl, hydrogen peroxide, ethephon, and salicylic acid treatments, the protein localization of BnNHL18A was changed. Our findings suggest a common function of BnNHL18A in biotic and abiotic stresses, and demonstrate the presence of the shared mechanism of protein translocalization between the responses to plant pathogen and to osmotic stress.

Original language	English
Pages (from-to)	399-406
Number of pages	8
Journal	Biochemical and biophysical research communications
Volume	339
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2005.10.210
Publication status	Published - 2006 Jan 6

Keywords

Brassica napus
HIN1
Hypersensitive response
NDR1
Osmotic stress
Sarcolipin

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2005.10.210

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title = "BnNHL18A shows a localization change by stress-inducing chemical treatments",

abstract = "The two genes, named BnNHL18A and BnNHL18B, showing sequence homology with Arabidopsis NDR1/HIN1-like (NHL) genes, were isolated from cDNA library prepared with oilseed rape (Brassica napus) seedlings treated with NaCl. The transcript level of BnNHL18A was increased by sodium chloride, ethephon, hydrogen peroxide, methyl jasmonate, or salicylic acid treatment. The coding regions of BnNHL18A and BnNHL18B contain a sarcolipin (SLN)-like sequence. Analysis of the localization of smGFP fusion proteins showed that BnNHL18A is mainly localized to endoplasmic reticulum (ER). This result suggests that the SLN-like sequence plays a role in retaining proteins in ER membrane in plants. In response to NaCl, hydrogen peroxide, ethephon, and salicylic acid treatments, the protein localization of BnNHL18A was changed. Our findings suggest a common function of BnNHL18A in biotic and abiotic stresses, and demonstrate the presence of the shared mechanism of protein translocalization between the responses to plant pathogen and to osmotic stress.",

keywords = "Brassica napus, HIN1, Hypersensitive response, NDR1, Osmotic stress, Sarcolipin",

author = "Lee, {Suk Bae} and Ham, {Byung Kook} and Park, {Jeong Mee} and Kim, {Young Jin} and Paek, {Kyung Hee}",

note = "Funding Information: This work was supported by a grant from BioGreen 21 Program, Rural Development Administration, Republic of Korea, and a grant from the Korea Science and Engineering Foundation to the Plant Signaling Network Research Center. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2006",

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doi = "10.1016/j.bbrc.2005.10.210",

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AU - Ham, Byung Kook

AU - Park, Jeong Mee

AU - Kim, Young Jin

AU - Paek, Kyung Hee

N1 - Funding Information: This work was supported by a grant from BioGreen 21 Program, Rural Development Administration, Republic of Korea, and a grant from the Korea Science and Engineering Foundation to the Plant Signaling Network Research Center. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2006/1/6

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AB - The two genes, named BnNHL18A and BnNHL18B, showing sequence homology with Arabidopsis NDR1/HIN1-like (NHL) genes, were isolated from cDNA library prepared with oilseed rape (Brassica napus) seedlings treated with NaCl. The transcript level of BnNHL18A was increased by sodium chloride, ethephon, hydrogen peroxide, methyl jasmonate, or salicylic acid treatment. The coding regions of BnNHL18A and BnNHL18B contain a sarcolipin (SLN)-like sequence. Analysis of the localization of smGFP fusion proteins showed that BnNHL18A is mainly localized to endoplasmic reticulum (ER). This result suggests that the SLN-like sequence plays a role in retaining proteins in ER membrane in plants. In response to NaCl, hydrogen peroxide, ethephon, and salicylic acid treatments, the protein localization of BnNHL18A was changed. Our findings suggest a common function of BnNHL18A in biotic and abiotic stresses, and demonstrate the presence of the shared mechanism of protein translocalization between the responses to plant pathogen and to osmotic stress.

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BnNHL18A shows a localization change by stress-inducing chemical treatments

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