Abstract
The two genes, named BnNHL18A and BnNHL18B, showing sequence homology with Arabidopsis NDR1/HIN1-like (NHL) genes, were isolated from cDNA library prepared with oilseed rape (Brassica napus) seedlings treated with NaCl. The transcript level of BnNHL18A was increased by sodium chloride, ethephon, hydrogen peroxide, methyl jasmonate, or salicylic acid treatment. The coding regions of BnNHL18A and BnNHL18B contain a sarcolipin (SLN)-like sequence. Analysis of the localization of smGFP fusion proteins showed that BnNHL18A is mainly localized to endoplasmic reticulum (ER). This result suggests that the SLN-like sequence plays a role in retaining proteins in ER membrane in plants. In response to NaCl, hydrogen peroxide, ethephon, and salicylic acid treatments, the protein localization of BnNHL18A was changed. Our findings suggest a common function of BnNHL18A in biotic and abiotic stresses, and demonstrate the presence of the shared mechanism of protein translocalization between the responses to plant pathogen and to osmotic stress.
| Original language | English |
|---|---|
| Pages (from-to) | 399-406 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 339 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2006 Jan 6 |
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Keywords
- Brassica napus
- HIN1
- Hypersensitive response
- NDR1
- Osmotic stress
- Sarcolipin
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
BnNHL18A shows a localization change by stress-inducing chemical treatments. / Lee, Suk B.; Ham, Byung Kook; Park, Jeong Mee; Kim, Young Jin; Paek, Kyung Hee.
In: Biochemical and Biophysical Research Communications, Vol. 339, No. 1, 06.01.2006, p. 399-406.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - BnNHL18A shows a localization change by stress-inducing chemical treatments
AU - Lee, Suk B.
AU - Ham, Byung Kook
AU - Park, Jeong Mee
AU - Kim, Young Jin
AU - Paek, Kyung Hee
PY - 2006/1/6
Y1 - 2006/1/6
N2 - The two genes, named BnNHL18A and BnNHL18B, showing sequence homology with Arabidopsis NDR1/HIN1-like (NHL) genes, were isolated from cDNA library prepared with oilseed rape (Brassica napus) seedlings treated with NaCl. The transcript level of BnNHL18A was increased by sodium chloride, ethephon, hydrogen peroxide, methyl jasmonate, or salicylic acid treatment. The coding regions of BnNHL18A and BnNHL18B contain a sarcolipin (SLN)-like sequence. Analysis of the localization of smGFP fusion proteins showed that BnNHL18A is mainly localized to endoplasmic reticulum (ER). This result suggests that the SLN-like sequence plays a role in retaining proteins in ER membrane in plants. In response to NaCl, hydrogen peroxide, ethephon, and salicylic acid treatments, the protein localization of BnNHL18A was changed. Our findings suggest a common function of BnNHL18A in biotic and abiotic stresses, and demonstrate the presence of the shared mechanism of protein translocalization between the responses to plant pathogen and to osmotic stress.
AB - The two genes, named BnNHL18A and BnNHL18B, showing sequence homology with Arabidopsis NDR1/HIN1-like (NHL) genes, were isolated from cDNA library prepared with oilseed rape (Brassica napus) seedlings treated with NaCl. The transcript level of BnNHL18A was increased by sodium chloride, ethephon, hydrogen peroxide, methyl jasmonate, or salicylic acid treatment. The coding regions of BnNHL18A and BnNHL18B contain a sarcolipin (SLN)-like sequence. Analysis of the localization of smGFP fusion proteins showed that BnNHL18A is mainly localized to endoplasmic reticulum (ER). This result suggests that the SLN-like sequence plays a role in retaining proteins in ER membrane in plants. In response to NaCl, hydrogen peroxide, ethephon, and salicylic acid treatments, the protein localization of BnNHL18A was changed. Our findings suggest a common function of BnNHL18A in biotic and abiotic stresses, and demonstrate the presence of the shared mechanism of protein translocalization between the responses to plant pathogen and to osmotic stress.
KW - Brassica napus
KW - HIN1
KW - Hypersensitive response
KW - NDR1
KW - Osmotic stress
KW - Sarcolipin
UR - http://www.scopus.com/inward/record.url?scp=28144454286&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=28144454286&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2005.10.210
DO - 10.1016/j.bbrc.2005.10.210
M3 - Article
C2 - 16298336
AN - SCOPUS:28144454286
VL - 339
SP - 399
EP - 406
JO - The BMJ
JF - The BMJ
SN - 0730-6512
IS - 1
ER -