Bone formation in a rat tibial defect model using carboxymethyl cellulose/BioC/bone morphogenic protein-2 hybrid materials

Sang Heon Song, Young Pil Yun, Hak Jun Kim, Kyeongsoon Park, Sung Eun Kim, Hae Ryong Song

Research output: Contribution to journalArticle

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Abstract

The objective of this study was to assess whether carboxymethyl cellulose- (CMC-) based hydrogel containing BioC (biphasic calcium phosphate (BCP); tricalcium phosphate (TCP): hydroxyapatite (Hap) = 70: 30) and bone morphogenic protein-2 (BMP-2) led to greater bone formation than CMC-based hydrogel containing BioC without BMP-2. In order to demonstrate bone formation at 4 and 8 weeks, plain radiographs, microcomputed tomography (micro-CT) evaluation, and histological studies were performed after implantation of all hybrid materials on an 8 mm defect of the right tibia in rats. The plain radiographs and micro-CT analyses revealed that CMC/BioC/BMP-2 (0.5 mg) led to much greater mineralization at 4 and 8 weeks than did CMC/BioC or CMC/Bio/BMP-2 (0.1 mg). Likewise, bone formation and bone remodeling studies revealed that CMC/BioC/BMP-2 (0.5 mg) led to a significantly greater amount of bone formation and bone remodeling at 4 and 8 weeks than did CMC/BioC or CMC/BioC/BMP-2 (0.1 mg). Histological studies revealed that mineralized bone tissue was present around the whole circumference of the defect site with CMC/BioC/BMP-2 (0.5 mg) but not with CMC/BioC or CMC/BioC/BMP-2 (0.1 mg) at 4 and 8 weeks. These results suggest that CMC/BioC/BMP-2 hybrid materials induced greater bone formation than CMC/BioC hybrid materials. Thus, CMC/BioC/BMP-2 hybrid materials may be used as an injectable substrate to regenerate bone defects.

Original languageEnglish
Article number230152
JournalBioMed Research International
Volume2014
DOIs
Publication statusPublished - 2014 Jan 1

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Carboxymethylcellulose Sodium
Hybrid materials
Osteogenesis
Rats
Bone
Bone and Bones
Defects
Proteins
X-Ray Microtomography
Bone Remodeling
Hydrogel
Tomography
Durapatite
Tibia

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Bone formation in a rat tibial defect model using carboxymethyl cellulose/BioC/bone morphogenic protein-2 hybrid materials. / Song, Sang Heon; Yun, Young Pil; Kim, Hak Jun; Park, Kyeongsoon; Kim, Sung Eun; Song, Hae Ryong.

In: BioMed Research International, Vol. 2014, 230152, 01.01.2014.

Research output: Contribution to journalArticle

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