Bone formation of middle ear cavity using biphasic calcium phosphate lyophilized with Escherichia coli-derived recombinant human bone morphogenetic protein 2 using animal model

Sung Eun Kim, Young Pil Yun, Hae Ryong Song, Kyung Hee Choi, Bo Hae Kim, Eun Kyeung Lee, Jae Jun Song

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective: The aim of the study was to analyze the value of Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2) coated biphasic calcium phosphate (BCP) for the obliteration of middle ear bone defect after mastoid surgery. Methods: Twenty-four specific pathogen-free Sprague-Dawley rats were randomly assigned to the BCP group ( n= 12) and BCP-ErhBMP-2 group ( n= 12; in which BCP scaffold of the granular type was coated with ErhBMP-2). In both groups, BCP scaffold was used to surgically fill the middle ear bulla. New bone formation was evaluated by measuring bone density (%) after 4 and 8 weeks in all rats in both groups. Results: At 4 weeks, new bone was visible at the periphery and center of the middle ear cavity in both groups. In the BCP group, a moderate amount of fibrous tissue had infiltrated into the interspace of the scaffolds. New bone almost totally filled the interspace in the BCP-ErhBMP-2 group. At 8 weeks, copious new bone formation had occurred. Histometric measurements showed that bone density in the BCP group was smaller than in the BCP-ErhBMP-2 group at 4 weeks (25.10% and 38.43%, respectively; p<. 0.05) and 8 weeks (25.54% and 34.18%, respectively; p<. 0.05). Conclusions: New bone formation was greater in the presence of BCP-ErhBMP-2 scaffolds. ErhBMP-2 coated BCP scaffolds is a potentially useful material for middle ear obliteration after mastoidectomy.

Original languageEnglish
Pages (from-to)1430-1433
Number of pages4
JournalInternational Journal of Pediatric Otorhinolaryngology
Volume77
Issue number9
DOIs
Publication statusPublished - 2013 Sep

Keywords

  • Bone morphogenetic protein
  • Mastoid obliteration
  • Otitis media
  • Scaffold

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Otorhinolaryngology

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