Bone marrow-derived cells or C-X-C motif chemokine 12 (CXCL12) treatment improve thin endometrium in a mouse model

Kyong Wook Yi, Ramanaiah Mamillapalli, Cagdas Sahin, Jaeyen Song, Reshef Tal, Hugh S. Taylor

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Successful implantation and pregnancy is dependent on sufficient endometrial growth during each reproductive cycle. Here, we report the therapeutic effect of either bone marrow-derived cells (BMDCs) or the stem cell chemo-attractant C-X-C motif chemokine 12 (CXCL12) on endometrial receptivity in a murine ethanol induced thin endometrium model. Endometrial epithelial area was significantly increased in mice treated with BMDCs, CXCL12, or by co-treatment with both compared with PBS-treated controls. Ki-67 and CD31 immunoreactivity was significantly higher in mice treated with either BMDCs, CXCL12, or both. The mRNA expression levels of endometrial receptivity markers leukemia inhibitory factor, interleukin-1β, and integrin beta-3 were increased in mice treated with either BMDCs, CXCL12, or both. The mRNA levels of matrix metalloproteinase-2 and -9 were significantly decreased by BMDCs but not by CXCL12. Pregnancy rates and litter size were increased after either treatment. Both BMDCs and CXCL12 displayed a comparable efficacy on endometrial regeneration in mice with thin endometrium. Our findings indicate the potential therapeutic effects of BMDCs and CXCL12 on infertility related to thin endometrium. Bone marrow-derived cells and CXCL12 displayed a comparable efficacy on endometrial regeneration in mice with thin endometrium.

Original languageEnglish
Pages (from-to)61-70
Number of pages10
JournalBiology of Reproduction
Volume100
Issue number1
DOIs
Publication statusPublished - 2019 Jan 1

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Chemokine CXCL12
CXC Chemokines
Endometrium
Bone Marrow Cells
Therapeutic Uses
Regeneration
Leukemia Inhibitory Factor
Litter Size
Messenger RNA
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Pregnancy Rate
Interleukin-1beta
Integrins
Infertility
Ethanol
Stem Cells
Pregnancy

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

Cite this

Bone marrow-derived cells or C-X-C motif chemokine 12 (CXCL12) treatment improve thin endometrium in a mouse model. / Yi, Kyong Wook; Mamillapalli, Ramanaiah; Sahin, Cagdas; Song, Jaeyen; Tal, Reshef; Taylor, Hugh S.

In: Biology of Reproduction, Vol. 100, No. 1, 01.01.2019, p. 61-70.

Research output: Contribution to journalArticle

Yi, Kyong Wook ; Mamillapalli, Ramanaiah ; Sahin, Cagdas ; Song, Jaeyen ; Tal, Reshef ; Taylor, Hugh S. / Bone marrow-derived cells or C-X-C motif chemokine 12 (CXCL12) treatment improve thin endometrium in a mouse model. In: Biology of Reproduction. 2019 ; Vol. 100, No. 1. pp. 61-70.
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