Bone marrow stromal cells promote neurite extension in organotypic spinal cord slice

Significance for cell transplantation therapy

Hideo Shichinohe, Satoshi Kuroda, Sachiko Tsuji, Satoshi Yamaguchi, Shunsuke Yano, Jang Bo Lee, Hiroyuki Kobayashi, Seiji Kikuchi, Kazutoshi Hida, Yoshinobu Iwasaki

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Objective. Recent reports have indicated that bone marrow stromal cells (BMSCs) have the potential to improve neurological function when transplanted into models of central nervous system (CNS) disorders, including traumatic spinal cord injury. In this study, the authors aimed to clarify the underlying mechanism through which BMSCs supported CNS regeneration in the spinal cord. Methods. The authors topically applied mouse BMSCs expressing green fluorescence protein (0.4-4 × 104 cells) on the organotypic spinal cord slice culture prepared from 6-day-old rat pups (n = 17). They were co-cultured for 3 weeks after the slice culture started, and the behavior of the applied BMSCs was serially observed using a fluorescence bioimaging technique. The authors completed a histological analysis at the end of the co-cultures and evaluated the profiles of the cultured BMSCs using microarray and immunocytochemistry techniques. Results. The fluorescence bioimaging showed that the BMSCs survived and made a cluster on the slice during the experiments. They also induced a morphological change in the slice within 48 hours of co-culture. Immunohistochemistry analysis showed that the BMSCs promoted a marked neurite extension toward their cluster and some of the BMSCs expressed Tuj-1, an early neuronal marker. Analysis by microarray and immunocytochemistry revealed that BMSCs highly expressed the matrix metalloproteinases (MMPs), stromal cell-derived factor-1, and its specific receptor CXCR4. Conclusions. These findings suggest that the donor BMSCs can support CNS regeneration due to their acquisition of a suitable environment for differentiation and promotion of neurite extension via MMPs and chemokines.

Original languageEnglish
Pages (from-to)447-457
Number of pages11
JournalNeurorehabilitation and Neural Repair
Volume22
Issue number5
DOIs
Publication statusPublished - 2008 Sep 1
Externally publishedYes

Fingerprint

Cell Transplantation
Neurites
Cell- and Tissue-Based Therapy
Mesenchymal Stromal Cells
Spinal Cord
Fluorescence
Immunohistochemistry
Coculture Techniques
Matrix Metalloproteinases
Central Nervous System
Spinal Cord Regeneration
CXCR4 Receptors
Chemokine CXCL12
Central Nervous System Diseases
Microarray Analysis
Spinal Cord Injuries
Chemokines
Regeneration
Cultured Cells

Keywords

  • Bone marrow stromal cell
  • Cell transplantation
  • Neurite extension
  • Neuronal regeneration
  • Organotypic spinal cord slice culture

ASJC Scopus subject areas

  • Rehabilitation
  • Neurology
  • Clinical Neurology

Cite this

Bone marrow stromal cells promote neurite extension in organotypic spinal cord slice : Significance for cell transplantation therapy. / Shichinohe, Hideo; Kuroda, Satoshi; Tsuji, Sachiko; Yamaguchi, Satoshi; Yano, Shunsuke; Lee, Jang Bo; Kobayashi, Hiroyuki; Kikuchi, Seiji; Hida, Kazutoshi; Iwasaki, Yoshinobu.

In: Neurorehabilitation and Neural Repair, Vol. 22, No. 5, 01.09.2008, p. 447-457.

Research output: Contribution to journalArticle

Shichinohe, H, Kuroda, S, Tsuji, S, Yamaguchi, S, Yano, S, Lee, JB, Kobayashi, H, Kikuchi, S, Hida, K & Iwasaki, Y 2008, 'Bone marrow stromal cells promote neurite extension in organotypic spinal cord slice: Significance for cell transplantation therapy', Neurorehabilitation and Neural Repair, vol. 22, no. 5, pp. 447-457. https://doi.org/10.1177/1545968308315596
Shichinohe, Hideo ; Kuroda, Satoshi ; Tsuji, Sachiko ; Yamaguchi, Satoshi ; Yano, Shunsuke ; Lee, Jang Bo ; Kobayashi, Hiroyuki ; Kikuchi, Seiji ; Hida, Kazutoshi ; Iwasaki, Yoshinobu. / Bone marrow stromal cells promote neurite extension in organotypic spinal cord slice : Significance for cell transplantation therapy. In: Neurorehabilitation and Neural Repair. 2008 ; Vol. 22, No. 5. pp. 447-457.
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AU - Yano, Shunsuke

AU - Lee, Jang Bo

AU - Kobayashi, Hiroyuki

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AU - Hida, Kazutoshi

AU - Iwasaki, Yoshinobu

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