Bone regeneration using MMP sensitive-hyaluronic acid based hydro gels

Jungju Kim, Sook Kim In, Jung Hwang Soon, Chul Kim Ho, Yongdoo Park, Kyung Sun

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Hyaluronic acid (170 kDa)-based hydrogel was synthesized using acrylated hyaluronic acid. Matrix metalloproteinase (MMP) sensitive hyaluronic acid-based hydrogels were prepared by conjugation with two different peptides: cell adhesion peptides containing integrin binding domains (Arg-Gly-Asp: RGD) and a cross-linker with MMP degradable peptides to mimic the remodeling characteristics of natural extracellular matrices (ECMs) by cell-derived MMPs. Mechanical properties of these hydrogels were evaluated with different weight percent (2.5 %wt and 3.5 %wt) by measuring elastic modulus, viscous modulus and swelling ratio. Human mesenchymal stem cells (hMSCs) were cultured in MMP-sensitive or insensitive hyaluronic acid-based hydrogels and/or immobilized cell adhesive RGD peptides in vitro. Actin staining and image analysis proved that cells cultured in the MMP-sensitive hydrogel with RGD peptides showed dramatic cell spreading and sprouting. For in vivo calvarial defect regeneration, 3 different samples (MMP insensitive hydrogel, MMP sensitive hydrogel, MMP sensitive hydrogel with BMP-2 and hMSC) were prepared. After a 4-week implantation, the Masson-Trichrome (MT) staining and micro CT scan results demonstrated that the MMP sensitive hydrogels with BMP-2 and hMSCs had the highest mature bone formation. This MMP sensitive-hyaluronic acid based hydrogel could be useful in tissue engineering by improving tissue defect regeneration and tissue remodeling.

Original languageEnglish
Title of host publicationNEBEC 2009 - Proceedings of the IEEE 35th Annual Northeast Bioengineering Conference
DOIs
Publication statusPublished - 2009
EventIEEE 35th Annual Northeast Bioengineering Conference, NEBEC 2009 - Boston, MA, United States
Duration: 2009 Apr 32009 Apr 5

Publication series

NameBioengineering, Proceedings of the Northeast Conference
ISSN (Print)1071-121X

Other

OtherIEEE 35th Annual Northeast Bioengineering Conference, NEBEC 2009
CountryUnited States
CityBoston, MA
Period09/4/309/4/5

ASJC Scopus subject areas

  • Bioengineering

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  • Cite this

    Kim, J., In, S. K., Soon, J. H., Ho, C. K., Park, Y., & Sun, K. (2009). Bone regeneration using MMP sensitive-hyaluronic acid based hydro gels. In NEBEC 2009 - Proceedings of the IEEE 35th Annual Northeast Bioengineering Conference [4967789] (Bioengineering, Proceedings of the Northeast Conference). https://doi.org/10.1109/NEBC.2009.4967789