Bone regeneration with hyalruronic acid based hydrogel-nanoparticle complex and rhBMP-2 in rat critical size defect model

Jong Ho Lee, Soung Min Kim, Jong Chul Park, Mi Ae Sung, Sang Bae Yu, Jeong Hun Nam, Yong Il Chung, Jung Ju Kim, Yong Doo Park, Giyoong Tae

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

As an efficient controlled release system of rhBMP-2, a functional nanoparticle-130 KDa 5% Hyaluronic Acid (HA) based hydrogel-Nanoparticle(NP) complex was developed and used as a bone transplant. In vivo bone formation was evaluated by soft x-ray, histology, alkaline phosphatase (ALP)/Osteocalcin activity and mineral contents analysis, based on the rat calvarial critical size defect (8 mm in diameter) model. Significantly, effective bone regeneration was achieved with the rhBMP-2 loaded HA based hydrogel-NP complex and the BMP-2 loaded HA based hydrogel as compared to only HA hydrogel group with the period of 4 weeks. These improved results included areas such as radiodensity, the bone specific ALP activity, the osteocalcin immunoreactivity, and the ratio of calcium and phosphate contents with respect to normal bone in the regenerated bone area. Although superiority of bone regenration in the rhBMP-2 loaded HA based hydrogel-NP complex group could not be confirmed statistically, all the evaluation parameters were higher in the rhBMP-2 loaded HA based hydrogel-NP complex group than in the the BMP-2 loaded HA based hydrogel group. These results indicate that the HA based hydrogel with nanoparticle complex can be a promising candidate for a new bone defect replacement matrix, and an enhanced BMP-2 scaffold.

Original languageEnglish
Pages (from-to)730-738
Number of pages9
JournalTissue Engineering and Regenerative Medicine
Volume6
Issue number4-11
Publication statusPublished - 2009 Jun

Keywords

  • BMP-2
  • Controlled release
  • Hyaluronic acid
  • Hydrogel
  • Nanoparticle

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Bone regeneration with hyalruronic acid based hydrogel-nanoparticle complex and rhBMP-2 in rat critical size defect model'. Together they form a unique fingerprint.

  • Cite this

    Lee, J. H., Kim, S. M., Park, J. C., Sung, M. A., Yu, S. B., Nam, J. H., Chung, Y. I., Kim, J. J., Park, Y. D., & Tae, G. (2009). Bone regeneration with hyalruronic acid based hydrogel-nanoparticle complex and rhBMP-2 in rat critical size defect model. Tissue Engineering and Regenerative Medicine, 6(4-11), 730-738.