Abstract
As an efficient controlled release system of rhBMP-2, a functional nanoparticle-130 KDa 5% Hyaluronic Acid (HA) based hydrogel-Nanoparticle(NP) complex was developed and used as a bone transplant. In vivo bone formation was evaluated by soft x-ray, histology, alkaline phosphatase (ALP)/Osteocalcin activity and mineral contents analysis, based on the rat calvarial critical size defect (8 mm in diameter) model. Significantly, effective bone regeneration was achieved with the rhBMP-2 loaded HA based hydrogel-NP complex and the BMP-2 loaded HA based hydrogel as compared to only HA hydrogel group with the period of 4 weeks. These improved results included areas such as radiodensity, the bone specific ALP activity, the osteocalcin immunoreactivity, and the ratio of calcium and phosphate contents with respect to normal bone in the regenerated bone area. Although superiority of bone regenration in the rhBMP-2 loaded HA based hydrogel-NP complex group could not be confirmed statistically, all the evaluation parameters were higher in the rhBMP-2 loaded HA based hydrogel-NP complex group than in the the BMP-2 loaded HA based hydrogel group. These results indicate that the HA based hydrogel with nanoparticle complex can be a promising candidate for a new bone defect replacement matrix, and an enhanced BMP-2 scaffold.
| Original language | English |
|---|---|
| Pages (from-to) | 730-738 |
| Number of pages | 9 |
| Journal | Tissue Engineering and Regenerative Medicine |
| Volume | 6 |
| Issue number | 4-11 |
| Publication status | Published - 2009 Jun 1 |
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Keywords
- BMP-2
- Controlled release
- Hyaluronic acid
- Hydrogel
- Nanoparticle
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Biomedical Engineering
Cite this
Bone regeneration with hyalruronic acid based hydrogel-nanoparticle complex and rhBMP-2 in rat critical size defect model. / Lee, Jong Ho; Kim, Soung Min; Park, Jong Chul; Sung, Mi Ae; Yu, Sang Bae; Nam, Jeong Hun; Chung, Yong Il; Kim, Jung Ju; Park, Yongdoo; Tae, Giyoong.
In: Tissue Engineering and Regenerative Medicine, Vol. 6, No. 4-11, 01.06.2009, p. 730-738.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Bone regeneration with hyalruronic acid based hydrogel-nanoparticle complex and rhBMP-2 in rat critical size defect model
AU - Lee, Jong Ho
AU - Kim, Soung Min
AU - Park, Jong Chul
AU - Sung, Mi Ae
AU - Yu, Sang Bae
AU - Nam, Jeong Hun
AU - Chung, Yong Il
AU - Kim, Jung Ju
AU - Park, Yongdoo
AU - Tae, Giyoong
PY - 2009/6/1
Y1 - 2009/6/1
N2 - As an efficient controlled release system of rhBMP-2, a functional nanoparticle-130 KDa 5% Hyaluronic Acid (HA) based hydrogel-Nanoparticle(NP) complex was developed and used as a bone transplant. In vivo bone formation was evaluated by soft x-ray, histology, alkaline phosphatase (ALP)/Osteocalcin activity and mineral contents analysis, based on the rat calvarial critical size defect (8 mm in diameter) model. Significantly, effective bone regeneration was achieved with the rhBMP-2 loaded HA based hydrogel-NP complex and the BMP-2 loaded HA based hydrogel as compared to only HA hydrogel group with the period of 4 weeks. These improved results included areas such as radiodensity, the bone specific ALP activity, the osteocalcin immunoreactivity, and the ratio of calcium and phosphate contents with respect to normal bone in the regenerated bone area. Although superiority of bone regenration in the rhBMP-2 loaded HA based hydrogel-NP complex group could not be confirmed statistically, all the evaluation parameters were higher in the rhBMP-2 loaded HA based hydrogel-NP complex group than in the the BMP-2 loaded HA based hydrogel group. These results indicate that the HA based hydrogel with nanoparticle complex can be a promising candidate for a new bone defect replacement matrix, and an enhanced BMP-2 scaffold.
AB - As an efficient controlled release system of rhBMP-2, a functional nanoparticle-130 KDa 5% Hyaluronic Acid (HA) based hydrogel-Nanoparticle(NP) complex was developed and used as a bone transplant. In vivo bone formation was evaluated by soft x-ray, histology, alkaline phosphatase (ALP)/Osteocalcin activity and mineral contents analysis, based on the rat calvarial critical size defect (8 mm in diameter) model. Significantly, effective bone regeneration was achieved with the rhBMP-2 loaded HA based hydrogel-NP complex and the BMP-2 loaded HA based hydrogel as compared to only HA hydrogel group with the period of 4 weeks. These improved results included areas such as radiodensity, the bone specific ALP activity, the osteocalcin immunoreactivity, and the ratio of calcium and phosphate contents with respect to normal bone in the regenerated bone area. Although superiority of bone regenration in the rhBMP-2 loaded HA based hydrogel-NP complex group could not be confirmed statistically, all the evaluation parameters were higher in the rhBMP-2 loaded HA based hydrogel-NP complex group than in the the BMP-2 loaded HA based hydrogel group. These results indicate that the HA based hydrogel with nanoparticle complex can be a promising candidate for a new bone defect replacement matrix, and an enhanced BMP-2 scaffold.
KW - BMP-2
KW - Controlled release
KW - Hyaluronic acid
KW - Hydrogel
KW - Nanoparticle
UR - http://www.scopus.com/inward/record.url?scp=80755149627&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80755149627&partnerID=8YFLogxK
M3 - Article
VL - 6
SP - 730
EP - 738
JO - Tissue Engineering and Regenerative Medicine
JF - Tissue Engineering and Regenerative Medicine
SN - 1738-2696
IS - 4-11
ER -