Both thermal and non-thermal stress protect against caerulein induced pancreatitis and prevent trypsinogen activation in the pancreas

J. L. Frossard, L. Bhagat, Hong Sik Lee, A. J. Hietaranta, V. P. Singh, A. M. Song, M. L. Steer, A. K. Saluja

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Background and aim: Recent studies have indicated that prior thermal stress causes upregulation of heat shock protein 70 (HSP70) expression in the pancreas and protects against secretagogue induced pancreatitis. The mechanisms responsible for the protective effect are not known. Similarly, the effects of prior non-thermal stress on HSP70 expression and pancreatitis are not known. The current studies were designed to specifically address these issues. Methods: In the current studies pancreatitis was induced by administration of a supramaximally stimulating dose of caerulein 12 hours after thermal stress and 24 hours after non-thermal (that is, β adrenergic stimulation) stress. Results: Both thermal and non-thermal stresses caused pancreatic HSP70 levels to rise and resulted in increased expression of HSP70 in acinar cells. Both forms of stresses protected against caerulein induced pancreatitis and prevented the early intrapancreatic activation of trypsinogen which occurs in this model of pancreatitis. Conclusions: These results suggest that both thermal and non-thermal stresses protect against pancreatitis by preventing intrapancreatic digestive enzyme activation and that HSP70 may mediate this protective effect.

Original languageEnglish
Pages (from-to)78-83
Number of pages6
JournalGut
Volume50
Issue number1
DOIs
Publication statusPublished - 2002 Jan 12
Externally publishedYes

Fingerprint

Trypsinogen
Ceruletide
HSP70 Heat-Shock Proteins
Pancreatitis
Pancreas
Hot Temperature
Enzyme Activation
Acinar Cells
Adrenergic Agents
Up-Regulation

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Both thermal and non-thermal stress protect against caerulein induced pancreatitis and prevent trypsinogen activation in the pancreas. / Frossard, J. L.; Bhagat, L.; Lee, Hong Sik; Hietaranta, A. J.; Singh, V. P.; Song, A. M.; Steer, M. L.; Saluja, A. K.

In: Gut, Vol. 50, No. 1, 12.01.2002, p. 78-83.

Research output: Contribution to journalArticle

Frossard, JL, Bhagat, L, Lee, HS, Hietaranta, AJ, Singh, VP, Song, AM, Steer, ML & Saluja, AK 2002, 'Both thermal and non-thermal stress protect against caerulein induced pancreatitis and prevent trypsinogen activation in the pancreas', Gut, vol. 50, no. 1, pp. 78-83. https://doi.org/10.1136/gut.0500078..
Frossard, J. L. ; Bhagat, L. ; Lee, Hong Sik ; Hietaranta, A. J. ; Singh, V. P. ; Song, A. M. ; Steer, M. L. ; Saluja, A. K. / Both thermal and non-thermal stress protect against caerulein induced pancreatitis and prevent trypsinogen activation in the pancreas. In: Gut. 2002 ; Vol. 50, No. 1. pp. 78-83.
@article{a88f2a43eebc495b958c20cd057fac00,
title = "Both thermal and non-thermal stress protect against caerulein induced pancreatitis and prevent trypsinogen activation in the pancreas",
abstract = "Background and aim: Recent studies have indicated that prior thermal stress causes upregulation of heat shock protein 70 (HSP70) expression in the pancreas and protects against secretagogue induced pancreatitis. The mechanisms responsible for the protective effect are not known. Similarly, the effects of prior non-thermal stress on HSP70 expression and pancreatitis are not known. The current studies were designed to specifically address these issues. Methods: In the current studies pancreatitis was induced by administration of a supramaximally stimulating dose of caerulein 12 hours after thermal stress and 24 hours after non-thermal (that is, β adrenergic stimulation) stress. Results: Both thermal and non-thermal stresses caused pancreatic HSP70 levels to rise and resulted in increased expression of HSP70 in acinar cells. Both forms of stresses protected against caerulein induced pancreatitis and prevented the early intrapancreatic activation of trypsinogen which occurs in this model of pancreatitis. Conclusions: These results suggest that both thermal and non-thermal stresses protect against pancreatitis by preventing intrapancreatic digestive enzyme activation and that HSP70 may mediate this protective effect.",
author = "Frossard, {J. L.} and L. Bhagat and Lee, {Hong Sik} and Hietaranta, {A. J.} and Singh, {V. P.} and Song, {A. M.} and Steer, {M. L.} and Saluja, {A. K.}",
year = "2002",
month = "1",
day = "12",
doi = "10.1136/gut.0500078..",
language = "English",
volume = "50",
pages = "78--83",
journal = "Gut",
issn = "0017-5749",
publisher = "BMJ Publishing Group",
number = "1",

}

TY - JOUR

T1 - Both thermal and non-thermal stress protect against caerulein induced pancreatitis and prevent trypsinogen activation in the pancreas

AU - Frossard, J. L.

AU - Bhagat, L.

AU - Lee, Hong Sik

AU - Hietaranta, A. J.

AU - Singh, V. P.

AU - Song, A. M.

AU - Steer, M. L.

AU - Saluja, A. K.

PY - 2002/1/12

Y1 - 2002/1/12

N2 - Background and aim: Recent studies have indicated that prior thermal stress causes upregulation of heat shock protein 70 (HSP70) expression in the pancreas and protects against secretagogue induced pancreatitis. The mechanisms responsible for the protective effect are not known. Similarly, the effects of prior non-thermal stress on HSP70 expression and pancreatitis are not known. The current studies were designed to specifically address these issues. Methods: In the current studies pancreatitis was induced by administration of a supramaximally stimulating dose of caerulein 12 hours after thermal stress and 24 hours after non-thermal (that is, β adrenergic stimulation) stress. Results: Both thermal and non-thermal stresses caused pancreatic HSP70 levels to rise and resulted in increased expression of HSP70 in acinar cells. Both forms of stresses protected against caerulein induced pancreatitis and prevented the early intrapancreatic activation of trypsinogen which occurs in this model of pancreatitis. Conclusions: These results suggest that both thermal and non-thermal stresses protect against pancreatitis by preventing intrapancreatic digestive enzyme activation and that HSP70 may mediate this protective effect.

AB - Background and aim: Recent studies have indicated that prior thermal stress causes upregulation of heat shock protein 70 (HSP70) expression in the pancreas and protects against secretagogue induced pancreatitis. The mechanisms responsible for the protective effect are not known. Similarly, the effects of prior non-thermal stress on HSP70 expression and pancreatitis are not known. The current studies were designed to specifically address these issues. Methods: In the current studies pancreatitis was induced by administration of a supramaximally stimulating dose of caerulein 12 hours after thermal stress and 24 hours after non-thermal (that is, β adrenergic stimulation) stress. Results: Both thermal and non-thermal stresses caused pancreatic HSP70 levels to rise and resulted in increased expression of HSP70 in acinar cells. Both forms of stresses protected against caerulein induced pancreatitis and prevented the early intrapancreatic activation of trypsinogen which occurs in this model of pancreatitis. Conclusions: These results suggest that both thermal and non-thermal stresses protect against pancreatitis by preventing intrapancreatic digestive enzyme activation and that HSP70 may mediate this protective effect.

UR - http://www.scopus.com/inward/record.url?scp=0036135383&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036135383&partnerID=8YFLogxK

U2 - 10.1136/gut.0500078..

DO - 10.1136/gut.0500078..

M3 - Article

VL - 50

SP - 78

EP - 83

JO - Gut

JF - Gut

SN - 0017-5749

IS - 1

ER -