Brain-derived neurotrophic factor regulation of N-methyl-D-aspartate receptor-mediated synaptic currents in suprachiasmatic nucleus neurons

Yang In Kim, H. J. Choi, C. S. Colwell

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Light information reaches the suprachiasmatic nucleus (SCN) through a subpopulation of retinal ganglion cells. Previous work raises the possibility that brain-derived neurotrophic factor (BDNF) and its high-affinity receptor TrkB may be important as modulators of this excitatory input into the SCN. To test this possibility, we used whole-cell patch-clamp methods to measure excitatory currents in rat SCN neurons. These currents were evoked by electrical stimulation of the optic nerve. We found that the amplitude of the N-methyl-D-aspartate (NMDA) component of the evoked excitatory postsynaptic currents (NMDA-EPSC) was increased by application of BDNF. The neurotrophin also increased the magnitude of NMDA-evoked currents in SCN neurons. The BDNF enhancement of the NMDA-EPSC was blocked by treatment with the neurotrophin receptor antagonist K252a as well as treatment with the soluble form of the TrkB receptor engineered as an immunoadhesin (TrkB IgG). Finally, the BDNF enhancement was lost in brain slices treated with the NR2B antagonist ifenprodil. The results demonstrate that BDNF and TrkB receptors are important regulators of retinal glutamatergic synaptic transmission within the SCN.

Original languageEnglish
Pages (from-to)1512-1520
Number of pages9
JournalJournal of Neuroscience Research
Volume84
Issue number7
DOIs
Publication statusPublished - 2006 Nov 15

Fingerprint

Suprachiasmatic Nucleus
trkB Receptor
Brain-Derived Neurotrophic Factor
N-Methyl-D-Aspartate Receptors
Neurons
N-Methylaspartate
Nerve Growth Factor Receptors
Retinal Ganglion Cells
Excitatory Postsynaptic Potentials
Nerve Growth Factors
Optic Nerve
Synaptic Transmission
Electric Stimulation
Immunoglobulin G
Light
Brain

Keywords

  • Circadian rhythms
  • NMDA
  • Rat
  • SCN
  • Suprachiasmatic nucleus
  • TrkB

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Brain-derived neurotrophic factor regulation of N-methyl-D-aspartate receptor-mediated synaptic currents in suprachiasmatic nucleus neurons. / Kim, Yang In; Choi, H. J.; Colwell, C. S.

In: Journal of Neuroscience Research, Vol. 84, No. 7, 15.11.2006, p. 1512-1520.

Research output: Contribution to journalArticle

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