Abstract
Resveratrol (RES) has been studied for its effects on the lifespan extension of Caenorhabditis elegans, but controversy still remains on its mechanism related with SIR-2. In this study, longevity assay was performed to confirm SIR-2-dependent lifespan extension of C. elgeans with RES and oxyresveratrol (OXY), an isomer of hydroxylated RES using loss-of-function mutants of C. elegans including sir-2.1 mutant. The results showed that OXY and RES significantly (P < 0.05) extended the lifespan of C. elegans compared with the control. OXY and RES also significantly (P < 0.05) increased the mRNA expression levels of sir-2.1 and aak-2 in a dose-dependent manner and increased the protein expression levels of SIR-2.1. OXY and RES treatment extended the lifespan in daf-16 loss-of-function mutants, which suggested that lifespan extension was not occurring via the activation of DAF-16. However, OXY and RES failed to extend the lifespan in loss-of-function mutants of sir-2.1 and aak-2. Therefore, OXY and RES extend the lifespan of C. elegans by overexpression of SIR-2.1, which is related to lifespan extension through calorie restriction and the AMP-activated protein kinase (AMPK) pathway, although this process is independent of the FOXO/DAF-16 pathway.
| Original language | English |
|---|---|
| Pages (from-to) | 1757-1763 |
| Number of pages | 7 |
| Journal | Experimental Biology and Medicine |
| Volume | 241 |
| Issue number | 16 |
| DOIs | |
| Publication status | Published - 2016 Oct 1 |
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Keywords
- Caenorhabditis elegans
- lifespan
- Oxyresveratrol
- resveratrol
- SIR-2.1
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Brief Communication : SIR-2.1-dependent lifespan extension of Caenorhabditis elegans by oxyresveratrol and resveratrol. / Lee, Jiyun; Kwon, Gayeung; Park, Jieun; Kim, Jeong Keun; Lim, Young Hee.
In: Experimental Biology and Medicine, Vol. 241, No. 16, 01.10.2016, p. 1757-1763.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Brief Communication
T2 - SIR-2.1-dependent lifespan extension of Caenorhabditis elegans by oxyresveratrol and resveratrol
AU - Lee, Jiyun
AU - Kwon, Gayeung
AU - Park, Jieun
AU - Kim, Jeong Keun
AU - Lim, Young Hee
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Resveratrol (RES) has been studied for its effects on the lifespan extension of Caenorhabditis elegans, but controversy still remains on its mechanism related with SIR-2. In this study, longevity assay was performed to confirm SIR-2-dependent lifespan extension of C. elgeans with RES and oxyresveratrol (OXY), an isomer of hydroxylated RES using loss-of-function mutants of C. elegans including sir-2.1 mutant. The results showed that OXY and RES significantly (P < 0.05) extended the lifespan of C. elegans compared with the control. OXY and RES also significantly (P < 0.05) increased the mRNA expression levels of sir-2.1 and aak-2 in a dose-dependent manner and increased the protein expression levels of SIR-2.1. OXY and RES treatment extended the lifespan in daf-16 loss-of-function mutants, which suggested that lifespan extension was not occurring via the activation of DAF-16. However, OXY and RES failed to extend the lifespan in loss-of-function mutants of sir-2.1 and aak-2. Therefore, OXY and RES extend the lifespan of C. elegans by overexpression of SIR-2.1, which is related to lifespan extension through calorie restriction and the AMP-activated protein kinase (AMPK) pathway, although this process is independent of the FOXO/DAF-16 pathway.
AB - Resveratrol (RES) has been studied for its effects on the lifespan extension of Caenorhabditis elegans, but controversy still remains on its mechanism related with SIR-2. In this study, longevity assay was performed to confirm SIR-2-dependent lifespan extension of C. elgeans with RES and oxyresveratrol (OXY), an isomer of hydroxylated RES using loss-of-function mutants of C. elegans including sir-2.1 mutant. The results showed that OXY and RES significantly (P < 0.05) extended the lifespan of C. elegans compared with the control. OXY and RES also significantly (P < 0.05) increased the mRNA expression levels of sir-2.1 and aak-2 in a dose-dependent manner and increased the protein expression levels of SIR-2.1. OXY and RES treatment extended the lifespan in daf-16 loss-of-function mutants, which suggested that lifespan extension was not occurring via the activation of DAF-16. However, OXY and RES failed to extend the lifespan in loss-of-function mutants of sir-2.1 and aak-2. Therefore, OXY and RES extend the lifespan of C. elegans by overexpression of SIR-2.1, which is related to lifespan extension through calorie restriction and the AMP-activated protein kinase (AMPK) pathway, although this process is independent of the FOXO/DAF-16 pathway.
KW - Caenorhabditis elegans
KW - lifespan
KW - Oxyresveratrol
KW - resveratrol
KW - SIR-2.1
UR - http://www.scopus.com/inward/record.url?scp=84988036819&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84988036819&partnerID=8YFLogxK
U2 - 10.1177/1535370216650054
DO - 10.1177/1535370216650054
M3 - Article
C2 - 27190265
AN - SCOPUS:84988036819
VL - 241
SP - 1757
EP - 1763
JO - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)
JF - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)
SN - 1535-3702
IS - 16
ER -