Bright ligand-activatable fluorescent protein for high-quality multicolor live-cell super-resolution microscopy

Jiwoong Kwon, Jong Seok Park, Minsu Kang, Soobin Choi, Jumi Park, Gyeong Tae Kim, Changwook Lee, Sangwon Cha, Hyun Woo Rhee, Sang Hee Shim

Research output: Contribution to journalArticle

Abstract

We introduce UnaG as a green-to-dark photoswitching fluorescent protein capable of high-quality super-resolution imaging with photon numbers equivalent to the brightest photoswitchable red protein. UnaG only fluoresces upon binding of a fluorogenic metabolite, bilirubin, enabling UV-free reversible photoswitching with easily controllable kinetics and low background under Epi illumination. The on- and off-switching rates are controlled by the concentration of the ligand and the excitation light intensity, respectively, where the dissolved oxygen also promotes the off-switching. The photo-oxidation reaction mechanism of bilirubin in UnaG suggests that the lack of ligand-protein covalent bond allows the oxidized ligand to detach from the protein, emptying the binding cavity for rebinding to a fresh ligand molecule. We demonstrate super-resolution single-molecule localization imaging of various subcellular structures genetically encoded with UnaG, which enables facile labeling and simultaneous multicolor imaging of live cells. UnaG has the promise of becoming a default protein for high-performance super-resolution imaging.

Original languageEnglish
Article number273
JournalNature communications
Volume11
Issue number1
DOIs
Publication statusPublished - 2020 Dec 1

Fingerprint

Microscopy
Microscopic examination
Ligands
microscopy
proteins
ligands
Imaging techniques
cells
Bilirubin
Proteins
emptying
Lighting
Photons
Protein Binding
Molecules
Covalent bonds
Photooxidation
metabolites
photooxidation
covalent bonds

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Bright ligand-activatable fluorescent protein for high-quality multicolor live-cell super-resolution microscopy. / Kwon, Jiwoong; Park, Jong Seok; Kang, Minsu; Choi, Soobin; Park, Jumi; Kim, Gyeong Tae; Lee, Changwook; Cha, Sangwon; Rhee, Hyun Woo; Shim, Sang Hee.

In: Nature communications, Vol. 11, No. 1, 273, 01.12.2020.

Research output: Contribution to journalArticle

Kwon, Jiwoong ; Park, Jong Seok ; Kang, Minsu ; Choi, Soobin ; Park, Jumi ; Kim, Gyeong Tae ; Lee, Changwook ; Cha, Sangwon ; Rhee, Hyun Woo ; Shim, Sang Hee. / Bright ligand-activatable fluorescent protein for high-quality multicolor live-cell super-resolution microscopy. In: Nature communications. 2020 ; Vol. 11, No. 1.
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