Broad-spectrum antiviral activity of 3d8, a nucleic acid-hydrolyzing single-chain variable fragment (Scfv), targeting sars-cov-2 and multiple coronaviruses in vitro

Gunsup Lee, Shailesh Budhathoki, Geum Young Lee, Kwang Ji Oh, Yeon Kyoung Ham, Young Jun Kim, Ye Rin Lim, Phuong Thi Hoang, Yongjun Lee, Seok Won Lim, Jun Mo Kim, Seungchan Cho, Tai Hyun Kim, Jin Won Song, Sukchan Lee, Won Keun Kim

Research output: Contribution to journalArticlepeer-review

Abstract

The virus behind the current pandemic, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the etiology of novel coronavirus disease (COVID-19) and poses a critical public health threat worldwide. Effective therapeutics and vaccines against multiple coronaviruses remain unavailable. Single-chain variable fragment (scFv), a recombinant antibody, exhibits broad-spectrum antiviral activity against DNA and RNA viruses owing to its nucleic acid-hydrolyzing property. The antiviral activity of 3D8 scFv against SARS-CoV-2 and other coronaviruses was evaluated in Vero E6 cell cultures. Viral growth was quantified with quantitative RT-qPCR and plaque assay. The nucleic acid-hydrolyzing activity of 3D8 was assessed through abzyme assays of in vitro viral transcripts and cell viability was determined by MTT assay. We found that 3D8 inhibited the replication of SARS-CoV-2, human coronavirus OC43 (HCoV-OC43), and porcine epidemic diarrhea virus (PEDV). Our results revealed the prophylactic and therapeutic effects of 3D8 scFv against SARS-CoV-2 in Vero E6 cells. Immunoblot and plaque assays showed the reduction of coronavirus nucleoproteins and infectious particles, respectively, in 3D8 scFv-treated cells. These data demonstrate the broad-spectrum antiviral activity of 3D8 against SARS-CoV-2 and other coronaviruses. Thus, it could be considered a potential antiviral countermeasure against SARS-CoV-2 and zoonotic coronaviruses.

Original languageEnglish
Article number650
JournalViruses
Volume13
Issue number4
DOIs
Publication statusPublished - 2021 Apr

Keywords

  • 3D8 scFv
  • COVID-19
  • Coronaviruses
  • SARS-CoV-2
  • Single-chain variable fragment

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

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