Bronchoalveolar cellularity and interleukin-8 levels in measles bronchiolitis obliterans

Young Yull Koh, Da Eun Jung, Ji Yeon Koh, Jung Yeon Kim, Young Yoo, Chang Keun Kim

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Background: Measles virus infection may progress to a chronic obstructive process including bronchiolitis obliterans (BO). This study investigates pulmonary cellular profiles and interleukin (IL)-8 levels in patients with BO following the measles. Methods: BAL fluid was obtained from 12 children with BO who had a history of measles pneumonia during an outbreak in 2000 and 2001. BAL cell counts and differentials were compared to control patients as well as BAL IL-8 levels, which were measured by enzyme-linked immunosorbent assay. Immunohistochemical staining of BAL cells and three open-lung biopsy specimens were also analyzed for T-cell surface markers CD3, CD4, and CD8. Results: BAL cellular profiles were characterized by a significantly increased percentage of neutrophils in the measles BO group (median, 16.0%) compared to the control group (2.3%) [p < 0.01]. BAL IL-8 levels were also markedly increased in the measles BO group (mean ± SD, 418.6 ± 286.0 pg/mL) compared to the control group (92.8 ± 126.7 pg/mL) [p < 0.01]. BAL IL-8 levels correlated significantly with neutrophil percentages in both the measles BO group (r = 0.86, p = 0.000) and the control group (r = 0.79, p = 0.007). The lymphocyte subsets were characterized by a significantly increased number of CD8+ cells, resulting in a decreased CD4/CD8 ratio in the BAL and the biopsy specimens. Conclusion: These results suggest that pulmonary neutrophils and IL-8, along with CD8+ T lymphocytes may play an important role in the pathogenesis of BO after measles virus infection.

Original languageEnglish
Pages (from-to)1454-1460
Number of pages7
JournalChest
Volume131
Issue number5
DOIs
Publication statusPublished - 2007 Jan 1

Fingerprint

Bronchiolitis Obliterans
Dimercaprol
Measles
Interleukin-8
Measles virus
Neutrophils
Virus Diseases
Lung
Control Groups
Cell Count
T-Lymphocytes
Biopsy
CD4-CD8 Ratio
Lymphocyte Subsets
Disease Outbreaks
Pneumonia
Enzyme-Linked Immunosorbent Assay
Staining and Labeling

Keywords

  • Bronchiolitis obliterans
  • CD8
  • Interleukin-8
  • Measles
  • Neutrophils

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine
  • Cardiology and Cardiovascular Medicine

Cite this

Koh, Y. Y., Jung, D. E., Koh, J. Y., Kim, J. Y., Yoo, Y., & Kim, C. K. (2007). Bronchoalveolar cellularity and interleukin-8 levels in measles bronchiolitis obliterans. Chest, 131(5), 1454-1460. https://doi.org/10.1378/chest.06-0188

Bronchoalveolar cellularity and interleukin-8 levels in measles bronchiolitis obliterans. / Koh, Young Yull; Jung, Da Eun; Koh, Ji Yeon; Kim, Jung Yeon; Yoo, Young; Kim, Chang Keun.

In: Chest, Vol. 131, No. 5, 01.01.2007, p. 1454-1460.

Research output: Contribution to journalArticle

Koh, Young Yull ; Jung, Da Eun ; Koh, Ji Yeon ; Kim, Jung Yeon ; Yoo, Young ; Kim, Chang Keun. / Bronchoalveolar cellularity and interleukin-8 levels in measles bronchiolitis obliterans. In: Chest. 2007 ; Vol. 131, No. 5. pp. 1454-1460.
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AB - Background: Measles virus infection may progress to a chronic obstructive process including bronchiolitis obliterans (BO). This study investigates pulmonary cellular profiles and interleukin (IL)-8 levels in patients with BO following the measles. Methods: BAL fluid was obtained from 12 children with BO who had a history of measles pneumonia during an outbreak in 2000 and 2001. BAL cell counts and differentials were compared to control patients as well as BAL IL-8 levels, which were measured by enzyme-linked immunosorbent assay. Immunohistochemical staining of BAL cells and three open-lung biopsy specimens were also analyzed for T-cell surface markers CD3, CD4, and CD8. Results: BAL cellular profiles were characterized by a significantly increased percentage of neutrophils in the measles BO group (median, 16.0%) compared to the control group (2.3%) [p < 0.01]. BAL IL-8 levels were also markedly increased in the measles BO group (mean ± SD, 418.6 ± 286.0 pg/mL) compared to the control group (92.8 ± 126.7 pg/mL) [p < 0.01]. BAL IL-8 levels correlated significantly with neutrophil percentages in both the measles BO group (r = 0.86, p = 0.000) and the control group (r = 0.79, p = 0.007). The lymphocyte subsets were characterized by a significantly increased number of CD8+ cells, resulting in a decreased CD4/CD8 ratio in the BAL and the biopsy specimens. Conclusion: These results suggest that pulmonary neutrophils and IL-8, along with CD8+ T lymphocytes may play an important role in the pathogenesis of BO after measles virus infection.

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